Detecting system capable of accurately acquiring ECL (electrochemiluminescence) spectral information

Abstract

The invention relates to a detecting system capable of accurately acquiring ECL (electrochemiluminescence) spectral information. The detecting system comprises an electrochemistry equipment unit, an ECL radiation generation device unit, a spectrum detection device unit and a data processing and control unit, wherein the ECL radiation generation device unit comprises an ECL cell as well as a working electrode, a reference electrode and a counter electrode which are connected with the electrochemistry equipment unit; the spectrum detection device unit comprises a collection objective lens and a CCD grating spectrometer; the ECL radiation generation device unit and the collection objective lens are arranged in a cassette, a luminous surface of the working electrode faces the collection objective lens, and the focus of the collection objective lens coincides with an entrance slit of the CCD grating spectrometer; the cassette is directly connected with a fixing plate of the entrance slit, and a detecting system light path is isolated from the outside; the CCD grating spectrometer and the electrochemistry equipment unit are triggered through TTL (transistor transistor logic) signals to realize synchronous operation, and the acquired electrochemical signals and ECL spectrum signals are synchronously input into the data processing and control unit.

Description

technical field [0001] The invention relates to a detection system capable of accurately collecting electrochemiluminescent spectrum information, belonging to the technical field of electrochemiluminescence detection. Background technique [0002] Electrochemiluminescence (ECL) has the advantages of both electrochemiluminescence and chemiluminescence. It is an analytical technique that uses electrochemical methods to excite a certain substance and make it produce light radiation. ECL analysis technology does not require an excitation light source, has the characteristics of high signal-to-noise ratio, sensitive detection and simple equipment, and has been widely used in biochemical analysis, medical diagnosis and other fields. ECL radiation is usually weak and unstable, which makes it difficult to obtain its spectral information using conventional fluorescence spectrum acquisition devices or wavelength scanning acquisition methods. Therefore, ECL detection devices usually us...

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Problems solved by technology

However, due to the particularity of the ECL radiation generation method resulting in low incident luminous flux, poor coordination between the electrochemical equipment and the CCD grating spectrometer, stray light interference, and the noise signal of the instrument itself, the above-mentioned direct use of the CCD grating spectrometer to collect ECL The method of spectroscopy not only has low signal-to-noise ratio and poor sensitivity, it cannot accurately collect the spectrum of weak ECL radiation, but also has poor reproducibility, and cannot accurately compare the spectral changes of ECL radiation under different electrochemical states or experimental conditions.

Therefore, the spectral ECL research based solely on the CCD grating spectrometer is also limited to the scope of simple qualitative analysis research.

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