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Method for differentiating pluripotent stem cell induced from mesenchymal stem cell into osteoblast

一种多能干细胞、质干细胞的技术，应用在将从间充质干细胞诱导的多能干细胞分化为造骨细胞领域，能够解决癌症危险性高、难度、卵子效率低等问题

Active Publication Date: 2016-06-22
BBHC
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the induced cells also have 2 pairs of genes, the cell fusion method has the problem of cell safety, and the somatic cell nuclear transfer method has the problem that a large number of eggs are required and the efficiency is very low
In addition, the specific factor injection method, as a method of using a virus containing an oncogene in order to insert a specific gene to induce dedifferentiation, has a high risk of inducing cancer, low efficiency, and difficulty in method operation, so it is widely used in cell therapy. There are problems with the possibility of development

Method used

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  • Method for differentiating pluripotent stem cell induced from mesenchymal stem cell into osteoblast
  • Method for differentiating pluripotent stem cell induced from mesenchymal stem cell into osteoblast
  • Method for differentiating pluripotent stem cell induced from mesenchymal stem cell into osteoblast

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Embodiment 1: the preparation of kelp extract

[0064] The crude drug samples to be used in the experiment were purchased in Jeju Island, South Korea, and were used in the experiment after accurate identification by experts. 100 g of the dried crude drug sample was placed in 1 liter of 70% methanol, and reflux extraction was performed for 16 hours, followed by filtration with filter paper. The filtrate was concentrated in a rotary evaporator and immediately lyophilized.

Embodiment 2

[0065] Example 2: Isolation of mesenchymal stem cells from human umbilical cord and culture of mesenchymal stem cells

Embodiment 2-1

[0066] Example 2-1: Collection of human umbilical cord

[0067]Umbilical cord tissue was collected directly postpartum. Before transferring the sample to the laboratory, after cleaning it up, immediately transfer it to the culture medium containing the addition of transfer medium (50 IU / ml penicillin, 50 μg / ml streptomycin (invitrogen, USA). Purchase 500ml sterilized glass bottles of F-12 medium in )). In the laboratory, stem cells are extracted under a sterile state, class 100 flow hood. The samples are first transferred to sterile stainless steel containers. After several washes with phosphate-buffered saline (PBS), the umbilical cord tissue samples were cut into 2 cm lengths and transferred to 10 cm diameter cell culture dishes, where additional washes were performed and anti-infection treated with 70% ethanol and treated with The phosphate-buffered saline solution added with antibiotic mixture (50 IU / ml penicillin, 50 μg / ml streptomycin (purchased from Invitrogen Life T...

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Abstract

The present invention relates to a medium composition containing an Ecklonia cava extract for dediffentiating an induced pluripotent stem cell. Also, the present invention relates to a method for differentiating an induced plurlipotent stem cell produced by using the medium composition into an osteoblast. When using the medium composition, according to the present invention, induced pluripotent stem cells using mesenchymal stem cells can be produced efficiently, and the pluripotent stem cells which have been produced can be useful as a cell treatment agent by being capable of being differentiated into osteoblasts.

Description

technical field [0001] The invention relates to a method for using a pluripotent stem cell induction medium composition of mesenchymal stem cells to prepare induced pluripotent stem cells and to differentiate the induced pluripotent stem cells into osteoblasts. Background technique [0002] Stem cells are a general term for undifferentiated cells that can be obtained in various tissues prior to differentiation. Stem cells have the property of being able to continuously produce cells identical to themselves within a predetermined period of time in an undifferentiated state, and of being able to differentiate into various cells that make up biological tissues under appropriate conditions. [0003] Stem cells can be broadly classified into embryonic stem cells and adult stem cells according to their differentiation ability and production period. Moreover, according to the differentiation ability of stem cells, they can also be divided into pluripotency, multipotency and unipot...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/02C12N5/0775
CPCC12N2500/76C12N2506/45C12N2501/39C12N5/0654C12N5/0696C12N2500/38C12N2500/42C12N2501/155C12N2506/1369A61P19/00A61K35/32C12N2501/999
Inventor 李尚燃郑元珠金浩彬吴珉善李桂浩
Owner BBHC
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