Method for rapidly detecting adenosine triphosphate (ATP) in iced chicken meat
A chilled and chicken technology, applied in material excitation analysis, fluorescence/phosphorescence, etc., can solve the problems of no standard method, inaccurate results, and ATP bioluminescence technology has not yet been found, and achieve the effect of small coefficient of variation
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0019] Material: chilled chicken.
[0020] Reagents: ATP disodium salt hydrate, phosphate buffer (1X), sterile water, ATP detection swab.
[0021] Instruments: handheld ATP fluorescence detector, Precelly24 homogenizer.
[0022] (1) ATP standard curve
[0023] ATP standard sample dilution: Dilute the ATP standard sample solution step by step to 10 with sterilized water -6 g / ml, 10 -7 g / ml, 10 -8 g / ml, 10 -9 g / ml, 10 -10 g / ml. Take a sample of 10 μl, then the moles of ATP are 0.2×10 -11 mol, 0.2×10 -12 mol, 0.2×10 -13 mol, 0.2×10 -14 mol, 0.2×10 -15 mol.
[0024] 1.2.2 Determination of ATP standard dilution sample Take 10 μl of ATP dilution solution with different gradients, put it on the ATP swab cotton swab, put on the coat, squeeze the reaction solution on it, react for 10 seconds, then put it into the portable ATP detector, and read after 10 seconds. Each sample was measured 3 times.
[0025] Make a standard curve according to the assay results of ATP standard...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com