Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method for detecting the viability of cistanche seeds

A technology of viability and cistanche, which is applied in the field of detection of viability of Cistanche seeds, can solve the problems of complex detection conditions and operations, high cost of chemical reagents, long detection cycle, etc.

Active Publication Date: 2019-01-01
INST OF BOTANY CHINESE ACAD OF SCI +1
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method of using biochemical principles to detect the viability of Cistanche seeds is not only expensive for chemical reagents, but also has a long detection cycle, complex detection conditions and operations, and is not suitable for quality assessment of large quantities of Cistanche seeds before artificial inoculation.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for detecting the viability of cistanche seeds
  • A method for detecting the viability of cistanche seeds
  • A method for detecting the viability of cistanche seeds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Embodiment 1, the method for detecting the viability of Cistanche seeds

[0059] 1. Cistanche seed selection

[0060] Cover the mature inflorescences of Cistanche deserticola that were normally strong in 2014 with three sections of white nylon mesh bags (the mesh size is greater than 70, to prevent the seeds from falling to the ground). After the seeds mature, collect the seeds from the inflorescences, mix the seeds with Evenly, pass it through soil sieves with apertures of 1.0, 0.8, 0.6, 0.4, and 0.2 mm in turn to obtain seeds with diameters of 0.8-1.0 mm, 0.6-0.8 mm, 0.4-0.6 mm, 0.2-0.4 mm, and 0-0.2 mm. mm seed at all levels. After absorbing water, the seeds of each diameter range were dissected under the dissecting microscope to observe the number of milky-white, plump, and solid embryos per 100 seeds of each level. Divide the number by 100 to obtain the percentage of seeds with viable embryos at each level. Seeds were repeated 4 times. The results of the determi...

Embodiment 2

[0086] Embodiment 2, the application of the method for detecting the viability of Cistanche seeds

[0087] 1. Select a group of seeds with a diameter of 0.4-1.0mm from a batch of Cistanche seeds

[0088] The 0-1.0mm seeds collected from the normal fruiting Cistanche infructome in 2014 are subjected to the following screening process:

[0089] 1) Pass a batch of Cistanche seeds through soil sieves with apertures of 1.0 and 0.4 mm to obtain seeds with a diameter of 0.4-1.0 mm;

[0090] 2) The seeds with a diameter of 0.4-1.0 mm are passed through soil sieves with an aperture of 1.0 and 0.5 mm to obtain seeds with a diameter of 0.5-1.0 mm;

[0091] 3) Pass the seeds with a diameter of 0.4-1.0 mm through soil sieves with apertures of 1.0 and 0.6 mm to obtain seeds with a diameter of 0.6-1.0 mm;

[0092] 4) Pass the seeds with a diameter of 0.6-1.0 mm through soil sieves with apertures of 1.0 and 0.8 mm to obtain seeds with a diameter of 0.8-1.0 mm.

[0093] Use a ten-thousandth...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for detecting vitality of cistanche seeds. The method comprises the following steps that seeds with the diameters ranging from 0.5 mm to 1.0 mm are separated from cistanche seeds of the same batch, and sampling is carried out with a quartering method to obtain sample seeds; the sample seeds are sequentially subjected to quick and full moisture soaking, morphological structure separation and in-vitro embryo microscopic examination, the percentage of seeds with vital embryos in the batch of seeds is counted and calculated, the batch of seeds are regarded as an effective batch of seeds if the value is larger than or equal to 77.5%, and the batch of seeds are regarded as an invalid batch of seeds if the value is smaller than 77.5%, wherein the effective batch of seeds are cistanche seeds with the appearance and quality basically uniform, high in vitality and capable of being directly used for field inoculation. The invention provides a seed quality detecting method and standard which are fast, easy and convenient to implement and low in cost in the cistanche industrial-scale planting process, and a technical support is provided for high-yield and efficient planting of cistanche.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for detecting the vitality of cistanche seeds. Background technique [0002] Cistanche deserticola (Cistanche deserticola Y.C.Ma) is a perennial holoparasitic herb belonging to the genus Cistanche (Orobanchaceae) in the family Orobanchaceae. At present, "Flora of my country" has identified five species and one variety of Cistanche in China, namely Cistanche, Cistanche (C.sinensis G.Beck), Salsa Cistanche [C.salsa (C.A.Mey.) G.Beck] ], tube flower Cistanche [C.tubulalosa (Schenk) Winht] and Lanzhou Cistanche (C.lanzhouensisZ.Y.Zhang), the suspected species is Mongolian Cistanche (C.mongolica). [0003] Cistanche is a rhizome medicinal material, which is a precious Chinese medicinal plant with a wide range of uses. It has been used as a strong and nourishing Chinese herbal medicine since ancient times. Cistanche has a medicinal history of more than 1,800 years in my country a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01C1/02
Inventor 黄振英王佳杨学军刘国方王柱芳梁守华张玉张金玉白生玉那木汗
Owner INST OF BOTANY CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com