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Method for detecting vitality of cistanche seeds

A technology for viability and cistanche deserticola is applied in the field of detecting the viability of Cistanche cistanche seeds, which can solve the problems of long detection period, complicated detection conditions and operations, and high cost of chemical reagents.

Active Publication Date: 2016-06-22
INST OF BOTANY CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method of using biochemical principles to detect the viability of Cistanche seeds is not only expensive for chemical reagents, but also has a long detection cycle, complex detection conditions and operations, and is not suitable for quality assessment of large quantities of Cistanche seeds before artificial inoculation.

Method used

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  • Method for detecting vitality of cistanche seeds
  • Method for detecting vitality of cistanche seeds
  • Method for detecting vitality of cistanche seeds

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Experimental program
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Effect test

Embodiment 1

[0058] Embodiment 1, the method for detecting the viability of Cistanche seeds

[0059] 1. Cistanche seed selection

[0060] Cover the mature inflorescences of Cistanche deserticola that were normally strong in 2014 with three sections of white nylon mesh bags (the mesh size is greater than 70, to prevent the seeds from falling to the ground). After the seeds mature, collect the seeds from the inflorescences, mix the seeds with Evenly, pass it through soil sieves with apertures of 1.0, 0.8, 0.6, 0.4, and 0.2 mm in turn to obtain seeds with diameters of 0.8-1.0 mm, 0.6-0.8 mm, 0.4-0.6 mm, 0.2-0.4 mm, and 0-0.2 mm. mm seed at all levels. After absorbing water, the seeds of each diameter range were dissected under the dissecting microscope to observe the number of milky-white, plump, and solid embryos per 100 seeds of each level. Divide the number by 100 to obtain the percentage of seeds with viable embryos at each level. Seeds were repeated 4 times. The results of the determi...

Embodiment 2

[0086] Embodiment 2, the application of the method for detecting the viability of Cistanche seeds

[0087] 1. Select a group of seeds with a diameter of 0.4-1.0mm from a batch of Cistanche seeds

[0088] The 0-1.0mm seeds collected from the normal fruiting Cistanche infructome in 2014 are subjected to the following screening process:

[0089] 1) Pass a batch of Cistanche seeds through soil sieves with apertures of 1.0 and 0.4 mm to obtain seeds with a diameter of 0.4-1.0 mm;

[0090] 2) The seeds with a diameter of 0.4-1.0 mm are passed through soil sieves with an aperture of 1.0 and 0.5 mm to obtain seeds with a diameter of 0.5-1.0 mm;

[0091] 3) Pass the seeds with a diameter of 0.4-1.0 mm through soil sieves with apertures of 1.0 and 0.6 mm to obtain seeds with a diameter of 0.6-1.0 mm;

[0092] 4) Pass the seeds with a diameter of 0.6-1.0 mm through soil sieves with apertures of 1.0 and 0.8 mm to obtain seeds with a diameter of 0.8-1.0 mm.

[0093] Use a ten-thousandth...

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Abstract

The invention discloses a method for detecting the vitality of cistanche seeds. The method comprises the following steps: separating seeds with a diameter of 0.5-1.0 mm from a batch of Cistanche seeds, and adopting a quartering method to carry out cuttings to obtain sample seeds. Sequentially make the sample seeds undergo rapid and sufficient water absorption, morphological structure separation and microscopic observation of isolated embryos, and count and calculate the percentage of seeds with viable embryos in the seed batch. If this value is equal to or greater than 77.50%, the batch of seeds It is regarded as a valid seed batch; if it is less than 77.50%, the batch of seeds is regarded as an invalid seed batch. The effective seed batches are Cistanche deserticola seeds with basically the same appearance and quality, high viability, and can be directly used for inoculation in the field. The invention proposes a fast, simple and low-cost method and standard for detecting the quality of seeds in the large-scale planting process of Cistanche deserticola, and provides technical support for high-yield and high-efficiency planting of Cistanche deserticola.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for detecting the vitality of cistanche seeds. Background technique [0002] Cistanche deserticola (Cistanchedeserticola Y.C.Ma) is a perennial holoparasitic herbaceous plant belonging to the genus Cistanche (Orobanchaceae) of the family Orobanchaceae. At present, "Flora of my country" has identified five species and one variety of Cistanche in China, namely Cistanche, Cistanche (C.sinensisG.Beck), Salsa Cistanche [C.salsa (C.A.Mey.) G.Beck] , Tube flower Cistanche [C.tubulalosa (Schenk) Winht] and Lanzhou Cistanche (C.lanzhouensisZ.Y.Zhang), the suspected species is Mongolian Cistanche (C.mongolica). [0003] Cistanche is a rhizome medicinal material, which is a precious Chinese medicinal plant with a wide range of uses. It has been used as a strong and nourishing Chinese herbal medicine since ancient times. Cistanche has a medicinal history of more than 1,800 years in my ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/02
Inventor 黄振英王佳杨学军刘国方王柱芳梁守华张玉张金玉白生玉那木汗
Owner INST OF BOTANY CHINESE ACAD OF SCI
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