AD or MCI detection marker and detection method thereof

A detection method and marker technology, applied in the field of disease detection, can solve the problem of lack of sensitive, specific and accurate biomarkers, etc.

Inactive Publication Date: 2016-06-08
SHENZHEN UNIV
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Problems solved by technology

[0007] In view of the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a kind of AD or MCI detection marker and its detection method, aiming to solve the problem that there is no sensitive, specific and accurate method for AD detection from peripheral blood in the prior art. or the question of biomarkers for MCI diagnosis

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  • AD or MCI detection marker and detection method thereof
  • AD or MCI detection marker and detection method thereof

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Embodiment 1

[0029] The serum of AD patients and their normal subjects of the same age were collected separately, and then miRCURY was used to TM The LNAArray gene chip was used to detect and compare miRNA expression profiles in the serum of AD patients and their normal counterparts. Results There were 92 miRNAs with differential expression fold ≥ 2.0, including 66 down-regulated miRNAs and 26 up-regulated miRNAs. From the above 92 differentially expressed miRNAs, 18 miRNAs with significant change folds were selected to further verify each serum sample by Q-PCR method. According to the verification results, 5 differentially expressed miRNAs and 5 reported miRNAs differentially expressed in patients with AD or MCI were selected for verification in a large number of serum samples using the miRNA detection technology S-Poly(T) method. Changes in expression levels for diagnosis.

[0030] The invention screens and verifies differentially expressed miRNAs in patient serum, and obtains markers ...

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Abstract

The invention discloses an AD or MCI detection marker and a detection method thereof. The AD or MCI detection marker is serum miRNA. The serum miRNA comprises hsa-miR-103a-3p, hsa-miR-15b-5p, hsa-let-7d-3p, hsa-let-7d-5p, hsa-miR-15a-5p and hsa-miR-150-5p. The AD or MCI detection marker has the advantages that the serum can be collected fast and simply, the AD or MCI detection marker is low in traumatic property and high in safety as compared with cerebrospinal fluid sampling, and the AD or MCI detection marker is free of radioactive damage to patients as compared to iconography manners such as PET; an miRCURYTM LNA Array gene chip is combined with the new S-Poly(T) method for miRNA detection, and method accuracy and sensitivity are increased; the detection of the expression quantity change of the miRNA has the advantage of quantitative detection as compared with scale detection and imaging diagnosis, subjectivity is lowered, and accuracy is achieved.

Description

technical field [0001] The invention relates to the technical field of disease detection, in particular to an AD or MCI detection marker and a detection method thereof. Background technique [0002] Alzheimer's disease (AD) is a kind of degenerative brain disease, clinically the main symptom is intellectual impairment. Most of the disease occurs in the elderly, the course of the disease progresses slowly, and the advanced disease is irreversible. With the aging of my country's population, the incidence of AD is increasing year by year. Severe cognitive decline and language impairment lead to AD patients' communication difficulties and inability to take care of themselves, which causes pain to patients, and also brings heavy burdens to families and society. It has become a serious social problem and has attracted governments all over the world. and the general concern of the medical community. Therefore, finding a fast and effective method for early diagnosis of AD is of g...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/113
CPCC12Q1/6883C12Q2600/158C12Q2600/178
Inventor 应明钟亦逸刘琼倪嘉缵
Owner SHENZHEN UNIV
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