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Processing method for anti-long-distance transportation of eucalyptus robusta Smith tissue culture seedlings

A technology of long-distance transportation and treatment method, which is applied in the field of eucalyptus tissue culture seedlings resistant to long-distance transportation, and can solve problems such as high production costs, heavy workload, and complicated work procedures

Inactive Publication Date: 2016-06-08
蒋凡
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a kind of eucalyptus test-tube seedling room anti-long-distance transportation cultivation method, take the eucalyptus test-tube seedling of 2-4cm as test material, adopt the outdoor hydroponics mode, after auxin 2,4-D naphthalene acetic acid NAA process, And carry out transplanting, have overcome the shortcomings such as complicated working procedures, heavy workload and high production cost in the process of inducing rooting of test-tube seedlings and seedling hardening and transplanting in the prior art

Method used

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  • Processing method for anti-long-distance transportation of eucalyptus robusta Smith tissue culture seedlings
  • Processing method for anti-long-distance transportation of eucalyptus robusta Smith tissue culture seedlings
  • Processing method for anti-long-distance transportation of eucalyptus robusta Smith tissue culture seedlings

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Embodiment 1

[0017] (1) Adopt eucalyptus test-tube seedlings through successive subcultures for 3 times, directly take out the eucalyptus test-tube seedlings from the culture bottle, wash the culture medium and put it into a stainless steel basin filled with water, cut the eucalyptus test-tube seedlings from the callus The lower specification is 3cm single eucalyptus test-tube seedling, remove the base leaves and wash the juice flowing out, and then insert the single test-tube seedling into the hole of the inverted 96-hole plug tray and put it into a square plate containing tap water for outdoor hydroponic experiments;

[0018] (2) During the outdoor hydroponic experiment, four treatments and three repetitions of the single-factor outdoor hydroponic rooting induction test were performed on the rubber tree test-tube seedlings, and the rooting induced by the medium in the culture room was used as a control. The specific method of hydroponic rooting induction is: the amount of tap water in the...

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Abstract

The invention discloses a processing method for anti-long-distance transportation of eucalyptus robusta Smith tissue culture seedlings. The processing method comprises the following steps: performing outdoor water culture test on the robust eucalyptus robusta Smith test tube seedlings; during water culture, controlling the quantity of tap water in a square plate not to be in contact with leaves, pouring out the tap water when the quantity of the tap water in the square plate is lower than 1 / 3 of eucalyptus robusta Smith test tube seedling stem sections, supplementing 2,4-D naphthylacetic acid (NAA), processing for 1-3 times, pouring out auxin in the square plate, supplementing pure tap water, culturing for 1-3 days, supplementing 2,4-D naphthylacetic acid again and replacing the auxin in the square plate every 4-7 days; performing water culture for 15-40 days, cleaning the auxin on the eucalyptus robusta Smith test tube seedlings, prohibiting splitting into monomers, directly placing the whole eucalyptus robusta Smith test tube seedlings in the square plate on a seedling tray filled with a transplantation medium, spreading dry red mud on the peripheries of the eucalyptus robusta Smith test tube seedlings for long-distance transportation. The method disclosed by the invention has the advantages that the eucalyptus robusta Smith seedlings early adapt to an outdoor growth mode, are resistant to lodge and vibrate and adapt to the environment, and pathogenic bacteria among the eucalyptus robusta Smith seedlings is reduced.

Description

technical field [0001] The invention relates to a long-distance transportation-resistant cultivation method for eucalyptus tissue culture seedlings. technical background [0002] Eucalyptus (Eucalyptus robusta Smith) is a plant of the family Myrtaceae. During the long-distance transportation of eucalyptus tissue culture bottle seedlings, the roots of the seedlings are perishable, and the survival rate after transplanting is low, which causes losses to the seedling farmers, and also restricts the long-distance transportation and short-term storage of eucalyptus tissue culture seedlings. Contents of the invention [0003] The technical problem to be solved by the invention is a treatment method for making eucalyptus tissue culture seedlings resistant to long-distance transportation. [0004] The object of the present invention is to provide a kind of eucalyptus test-tube seedling room anti-long-distance transportation cultivation method, take the eucalyptus test-tube seedli...

Claims

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Application Information

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IPC IPC(8): A01G31/00
CPCA01G31/00
Inventor 蒋凡
Owner 蒋凡
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