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Black garlic extract and application thereof in preventing and curing tongue cancer

An extract and technology of black garlic, applied in the field of black garlic extract and its prevention and treatment of tongue cancer, achieves the effects of changing cell morphology, simple preparation method, and increasing soluble solid content

Inactive Publication Date: 2016-05-25
广东益生康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no report on the use of black garlic extract in the prevention and treatment of tongue cancer

Method used

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  • Black garlic extract and application thereof in preventing and curing tongue cancer
  • Black garlic extract and application thereof in preventing and curing tongue cancer
  • Black garlic extract and application thereof in preventing and curing tongue cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The preparation of embodiment 1 black garlic extract

[0026] Take black garlic slices, add water 3 times the weight of the black garlic, extract at 85°C for 2 hours, filter, collect the first filtrate, and keep the filter residue; add water 1 times the weight of the black garlic to the filter residue, add the 0.003 times the weight of the black garlic compound plant hydrolase, enzymatic hydrolysis at 36 ° C for 1 hour, then add 1 times the weight of the black garlic 65% ethanol solution, heat and reflux for extraction for 3 hours, filter the filtrate, concentrate under reduced pressure, remove The second filtrate is obtained after ethanol; the first filtrate and the second filtrate are combined and mixed to obtain the black garlic extract. The soluble solid content of black garlic extract was 19.8%.

Embodiment 2

[0027] The preparation of embodiment 2 black garlic extract

[0028] Take black garlic slices, add water 5 times the weight of the black garlic, extract at 100°C for 3 hours, filter, collect the first filtrate, and keep the filter residue; add water 2 times the weight of the black garlic to the filter residue, add the The compound plant hydrolase of 0.004 times the weight of the black garlic is enzymolyzed at 40°C for 2 hours, then 75% ethanol solution of 2 times the weight of the black garlic is added, heated and refluxed for 4 hours, the filtrate is filtered, concentrated under reduced pressure, and removed The second filtrate is obtained after ethanol; the first filtrate and the second filtrate are combined and mixed to obtain the black garlic extract. The soluble solid content of black garlic extract was 19.2%.

Embodiment 3

[0029] The preparation of embodiment 3 black garlic extract

[0030] Take black garlic slices, add water 6 times the weight of the black garlic, extract at 115°C for 4 hours, filter, collect the first filtrate, and keep the filter residue; add water 2 times the weight of the black garlic to the filter residue, add the The compound plant hydrolase of 0.05 times the weight of black garlic is enzymolyzed at 45 ° C for 3 hours, then 85% ethanol solution of 3 times the weight of the black garlic is added, heated and refluxed for 6 hours, the filtrate is filtered, concentrated under reduced pressure, and removed The second filtrate is obtained after ethanol; the first filtrate and the second filtrate are combined and mixed to obtain the black garlic extract. The soluble solid content of black garlic extract was 18.7%.

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Abstract

The invention provides a black garlic extract which is prepared by the following steps that black garlic slices are taken, water with the weight of 3-6 times of that of black garlic is added to the black garlic, extraction is performed at the temperature of 85 DEG C to 115 DEG C by 2-4 parts, the mixture is filtered, first filtrate is collected, and filter residue is kept; water with the weight of 1-2 times of that of black garlic is added to the filter residue, composite plant hydrolases with the weight of 0.003-0.05 time of that of black garlic is added to the mixture, enzymolysis is performed, 60%-80% of ethanol solution with the weight of 1-3 times of that of black garlic is added, heating reflux extraction is performed by 3-6 h, filtering is performed to take filtrate, decompress concentration is performed, and second filtrate is obtained after ethyl alcohol removal; the first filtrate and the second filtrate are combined, and the black garlic extract is obtained after mixing. The black garlic extract has an inhibiting effect on growth of the tongue cancer Tc-8113 cell line and can be used for preventing and curing tongue cancer.

Description

technical field [0001] The invention relates to a black garlic extract and its application in preventing and treating tongue cancer. Background technique [0002] According to the strict anatomical definition, cancer of the tongue (carcinoma of tongue) should be divided into tongue body cancer (front 2 / 3 of the tongue) and tongue root cancer (back 1 / 3 of the tongue). Long-term smoking, drinking, chewing betel nuts, or wearing inappropriate dentures will cause repeated damage, hyperemia, and hyperplasia of the oral mucosa. Repeated damage and hyperplasia of oral mucosal cells will gradually increase the metabolism of the nucleus. The G1 phase of the entire cell proliferation cycle Cells continue to enter the S phase (DNA synthesis phase), prompting an increase in the number of cells in the S phase. After several years or decades (oral tissue damage reaches millions, or even more), the DNA content will eventually increase abnormally, resulting in chromosomal abnormalities and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/8962A61P35/00A23L33/105
CPCA61K36/8962A23V2002/00A61K2236/19A61K2236/333A23V2200/308A23V2250/21
Inventor 樊曙明
Owner 广东益生康生物科技有限公司
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