A kind of closed pathogen detection cartridge

Abstract

The invention provides a closed pathogen detection cassette which comprises a cavity, a sliding blade arranged on the closed cavity, a pipette assembly combined with the sliding blade, a button lid, and a pedestal which is matched and combined with the cavity. The cassette can complete the whole pathogen detection process (including nucleic acid extraction, amplification and detection) in a closed environment, and is suitable for automatic control. The cassette can effectively avoid the problem of pollution in amplification detection of pathogenic microorganisms and the like, the problem of infection of the experiment operators, and other problems.

Description

technical field [0001] The invention relates to a closed pathogen detection cartridge, which is suitable for a portable and automatic biochemical detection system and belongs to the fields of biology, analytical chemistry and medical detection. Background technique [0002] Nucleic acid detection has been widely used in biomedicine and other fields. The entire process of traditional nucleic acid detection (including nucleic acid extraction, PCR amplification, and detection) needs to be operated on different instruments, and samples need to be transferred multiple times, which greatly increases the chance of cross-contamination of samples, especially from PCR amplification. Contamination occurred during the operation of taking samples after opening the cover. As a result, the final test result is inaccurate, which brings very serious harm to patients. Although a series of methods have been developed to prevent cross-contamination of amplification products, such as strict pa...

AI Technical Summary

Problems solved by technology

Most of these instruments are foreign instruments, which are very expensive and not suitable for promotion, especially in Africa and other places where diseases are prone to outbreaks.

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