Fusarium oxysporum nucleotide sequence qualitative standard sample and preparing method thereof

Abstract

The invention belongs to the field of biotechnology, and particularly relates to a fusarium oxysporum nucleotide sequence qualitative standard sample and a preparing method thereof. Fusarium oxysporum in the logarithmic phase is acquired, total DNA extraction is conducted, specific PCR amplification sequencing is conducted, and then plasmid preparation is conducted. The prepared nucleotide sequence standard sample does not have biological activity any more, biological pollution and infection are avoided, the source of molecular biology positive control is available, and the sample is stable, free of pollution and easy to store. Based on the fact that fusarium oxysporum has the nucleotide sequence technical index with taxonomical significance, the problems of fusarium oxysporum nucleotide sequence standard sample source tracing reference system and reference index are solved, the fusarium oxysporum nucleotide sequence standard sample with real source tracing significance is obtained through application in setting value analysis of the standard sample, and the fusarium oxysporum nucleotide sequence qualitative standard sample has great significance in development of nucleotide sequence classified gradation standard samples in China.

Description

technical field [0001] The invention belongs to the field of biological technology, in particular to a qualitative standard sample of nucleic acid sequence of Fusarium oxysporum and a preparation method thereof. Background technique [0002] The World Food and Agriculture Organization (FAO) reports that about 25% of the world's crops are polluted by fungi and their mycotoxins every year, and about 2% of the crops lose their nutritional value and economic value due to serious pollution. Direct and indirect losses caused by pollution may reach tens of billions of dollars. [0003] Among the currently known mycotoxins, Fusarium (Fusarium) is an important plant pathogen that harms crops such as wheat, corn, rice, and vegetables, causing severe production reduction. The zearalenone toxin produced by Fusarium toxin It has obvious cytotoxicity and inhibitory effect on protein and DNA synthesis, and has certain teratogenicity and mutagenicity, and the pollution is very extensive. ...

AI Technical Summary

Problems solved by technology

The status of traceability description of classification and grading national standard samples shows that people's awareness and understanding of the traceability of such standard samples is in a relatively chaotic stage, and there is no unified specification

Images