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Method for increasing fermentation output of avilamycin

A technology of avelamycin and yield, applied in the field of biopharmaceuticals, can solve the problems of low potency, low components A and B of avelamycin, no feeding and variable temperature culture, etc., to improve Yield, the effect of increasing fermentation yield

Inactive Publication Date: 2016-05-11
山东胜利生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The application number is 200910017738.8, and the invention title is "the strain and method for producing the special antibiotic avilamycin for animals". The fermentation method used in the invention patent does not carry out feeding and variable temperature culture, and the titer is low. Avilamycin Element A and B components are low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] 1) Get the strain and inoculate it into the seed culture medium, the inoculum size is 25mm 2 The slant, the shaker with an amplitude of 5 cm and a rotation speed of 220 rpm, cultured at 28.0° C. for 24 hours, and the pH value of the shaker seed medium was 6.40.

[0037] The strain is Streptomyces viridochromogenes, whose Latin literary name is streptomycesviridochromogenes, and is preserved in the General Microorganism Center of China Committee for the Collection of Microorganisms, with a preservation date of July 21, 2009, and a preservation number of CGMCCNo.3198.

[0038] The seed culture medium used is based on the percentage of the total weight of raw materials, and consists of the following raw materials: 1.5% of low-temperature soybean cake powder, 0.25% of dry yeast, 0.25% of glucose, 1.0% of dextrin, 2.0% of calcium chloride, and 0.1% of calcium carbonate and water balance;

[0039] 2) Inoculate the strains cultivated in 1) into the fermentation medium. The in...

Embodiment 2

[0047] 1) Get the strain and inoculate it into the seed culture medium, the inoculum size is 25mm 2 The slant, the shaker with an amplitude of 5 cm and a rotation speed of 230 rpm were cultivated at 28.0° C. for 25 hours, and the pH value of the shaker seed medium was 6.50.

[0048] The strain is Streptomyces viridochromogenes, whose Latin literary name is streptomycesviridochromogenes, and is preserved in the General Microorganism Center of China Committee for the Collection of Microorganisms, with a preservation date of July 21, 2009, and a preservation number of CGMCCNo.3198.

[0049] The seed culture medium used is based on the percentage of the total weight of raw materials, and is composed of the following raw materials: 1.75% of low-temperature soybean cake powder, 0.25% of dry yeast, 0.35% of glucose, 1.5% of dextrin, 2.5% of calcium chloride, and 0.15% of calcium carbonate and water balance;

[0050] 2) Inoculate the strains cultivated in 1) into the fermentation med...

Embodiment 3

[0058] 1) Get the strain and inoculate it into the seed culture medium, the inoculum size is 25mm 2 The slant, the shaker with an amplitude of 5 cm and a rotation speed of 250 rpm were cultivated at 29.0° C. for 26 hours, and the pH value of the seed medium was 6.70.

[0059] The strain is Streptomyces viridochromogenes, whose Latin literary name is streptomycesviridochromogenes, and is preserved in the General Microorganism Center of China Committee for the Collection of Microorganisms, with a preservation date of July 21, 2009, and a preservation number of CGMCCNo.3198.

[0060] The seed culture medium used is based on the percentage of the total weight of the raw materials, and is composed of the following raw materials: 2.0% of low-temperature soybean cake powder, 0.3% of dry yeast, 0.5% of glucose, 2.0% of dextrin, 3.0% of calcium chloride, and 0.2% of calcium carbonate and water balance;

[0061] 2) Inoculate the strains cultivated in 1) into the fermentation medium. Th...

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PUM

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Abstract

The invention relates to a method for increasing the fermentation output of avilamycin. The method comprises the following steps: taking a bacterial strain, inoculating the bacterial strain to a seed culture medium, wherein the bacterial strain is named as streptomyces viridochromogenes, is preserved in China General Microbiological Culture Collection Center (CGMCC) in 21st September, 2009, and the perseveration number is CGMCC No.3198; inoculating the cultured bacterial strain to a fermentation culture medium, wherein the inoculation quantity accounts for 10 to 13% of the mass of fermentation culture medium; carrying out fermentation for 20 to 24 hours, adding 6 to 8 wt% of L-valine solution, during the fermentation process, flowingly adding a carbon source (dextrin) and a nitrogen source (ammonium sulfate) into the fermentation broth, wherein the pH value of the fermentation culture medium is 7.00 to 7.20, the gas throughput is 0.6 to 1.3 vvm, and the rotation speed is 200 to 400 rpm, and carrying out variable temperature fermentation for 210 to 220 hours.

Description

technical field [0001] The invention relates to a method for increasing the fermentation yield of avelamycin, belonging to the technical field of biopharmaceuticals. Background technique [0002] Avilamycin (Avilamycin), also known as bemcicycline, belongs to oligosaccharide antibiotics and is a kind of animal-specific antibiotics with excellent use effect. Avelamycin has about 12 components such as A, B, C, D, E, F, G, H, etc., among which the most active component is Avelamycin A, which has a relatively high High safety, no absorption in the intestinal tract, no residue in livestock products, safe for the environment after excretion (including decomposition products); in the production of compound feed, it rarely produces dust and other pollution; it can be added to livestock and poultry feed , no withdrawal period is required. Due to the excellent use effect, avelamycin has been widely used in more than 40 countries in the world. [0003] The application number is 2009...

Claims

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Application Information

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IPC IPC(8): C12P19/60C12N1/20C12R1/465
CPCC12N1/20C12P19/60
Inventor 张善飞关志勇高鹏刘正光王丹丹赵才兵刘波王甜
Owner 山东胜利生物工程有限公司
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