Method for preparing semen raphani separation protein powder and hydrolysis concentrated solution
A technology for separating proteins and raisins, applied in the field of vegetable protein, can solve the problems of difficulty in separating ethanol-water azeotrope, high consumption of high-concentration ethanol, and high production cost, and achieves simple and easy recovery process, low production cost, and easy operation. easy effect
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Embodiment 1
[0027] 1. Preparation of radish seed residue
[0028] Be that the volume (L) ratio of 3 citric acid solutions is the ratio of 1: 4 according to defatted radish seed powder quality (kg) and pH, this citric acid solution is pumped into stainless steel hydrolysis tank earlier, open agitator and circulating hot water, wait When the citric acid solution was heated to 30°C. Add defatted radish seed powder, and then stir and hydrolyze at 30° C. for 4 hours. After the hydrolysis is completed, the hydrolyzate is discharged into the disc centrifuge, and the first centrifugation is carried out under the condition of 5000r / min, and the first hydrolysis centrifugation residue and the supernatant of the hydrolysis centrifugation are collected respectively. For the first hydrolysis and centrifugation slag collected, pump it into the washing kettle with a screw pump, add pure water to stir and wash twice, each washing time is 15 minutes, and the volume of pure water added during each washing...
Embodiment 2
[0040] 1. Preparation of radish seed residue
[0041] With embodiment 1, feature is: prepare the citric acid solution that pH is 3.5, be the ratio of 1: 6 according to the volume (L) ratio of defatted radish seed powder quality (kg) and citric acid solution, treat that citric acid solution is warming up to At 35°C, stirred and hydrolyzed at 35°C for 6 hours, centrifuged at 5500r / min, each washing time was 20 minutes, and centrifuged at 5500r / min.
[0042] 2. Preparation of Radix Seed Isolate Protein Extract
[0043] With embodiment 1, feature is: prepare the citric acid solution that pH is 6, the raspberry residue quality (kg) that collects by the 1st step and the ratio of citric acid solution volume (L) ratio are 1: 6, treat citric acid When the temperature of the solution was raised to 55°C, it was stirred and extracted at 55°C for 4 hours. Carry out centrifugal separation under the condition of 5500r / min, the addition volume of citric acid solution, pH value, concentratio...
Embodiment 3
[0053] 1. Preparation of radish seed residue
[0054] With embodiment 1, feature is: prepare the citric acid solution that pH is 4, be warming up to 40 ℃ according to the ratio of the volume (L) ratio of defatted radish seed powder quality (kg) and the volume (L) of citric acid solution of 1: 8, in Stir and hydrolyze at 40°C for 8 hours, then centrifuge at 6000r / min, wash for 30 minutes each time, and centrifuge at 6000r / min.
[0055] 2. Preparation of Radix Seed Isolate Protein Extract
[0056] With embodiment 1, feature is: prepare the citric acid solution that pH is 6.5, the raspberry residue mass (kg) that collects by the 1st step and the ratio of citric acid solution volume (L) ratio are 1: 8, treat citric acid When the temperature of the solution was raised to 65°C, it was stirred and extracted at 65°C for 5 hours. Carry out centrifugation under the condition of 6000r / min, the addition volume of citric acid solution, pH value, concentration, extraction time and the con...
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