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Hatching culture medium of artemia cyst, and preparation method and application thereof

A technology of culture medium and worm eggs, which is applied in the field of incubation culture medium and preparation of Artemia eggs, can solve problems such as non-universal significance, and achieve the effects of improving hatching efficiency and promoting hatching.

Inactive Publication Date: 2016-05-04
XIAMEN JINYIHAI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the optimal parameters obtained by the above methods are not universal, because the optimal parameters vary for different Artemia strains or different batches of Artemia eggs of the same strain

Method used

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  • Hatching culture medium of artemia cyst, and preparation method and application thereof
  • Hatching culture medium of artemia cyst, and preparation method and application thereof
  • Hatching culture medium of artemia cyst, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A hatching medium for Artemia eggs, including the following ingredients by weight:

[0027]

[0028] The preparation method includes the following processing steps:

[0029] (1) Add 20g NaCl, 15gKCl, 30gMgSO 4 , 10gSrCl 2 Dissolved in 1000g of H 2 In O, stir evenly to obtain a mixed solution;

[0030] (2) Sterilize the mixture prepared in step (1) under ultraviolet light for 20 minutes;

[0031] (3) Add 0.1g glucose and 0.05g glucose oxidase to the sterilized mixed solution in sequence, and stir to dissolve;

[0032] (4) Add 2g of vitamin C sodium and 0.5g of tea polyphenols, and stir evenly at 45℃;

[0033] (5) Centrifuge the material obtained in step (4) at a speed of 300r / min for 15 minutes, remove the sediment, and take the clear liquid;

[0034] (6) Add 22 g of H with a mass concentration of 30% to the clear solution prepared in step (5) 2 O 2 , The culture medium is obtained, and then the culture medium is quickly transferred to a closed transparent container.

[0035] The app...

Embodiment 2

[0037] A hatching medium for Artemia eggs, including the following ingredients by weight:

[0038]

[0039]

[0040] Wherein, the NaCl, KCl, MgSO 4 , SrCl 2 It is a chemically pure solid.

[0041] The preparation method includes the following processing steps:

[0042] (1) Mix 23g of NaCl, 17gKCl, 35gMgSO 4 , 12gSrCl 2 Dissolved in 1000g of H 2 In O, stir evenly to obtain a mixed solution;

[0043] (2) Sterilize the mixture prepared in step (1) under ultraviolet light for 25 minutes;

[0044] (3) Add 0.1g glucose and 0.07g glucose oxidase to the sterilized mixed solution in sequence, and stir to dissolve;

[0045] (4) Add 2g of vitamin C sodium and 0.6g of tea polyphenols, and stir evenly at 50℃;

[0046] (5) Centrifuge the material prepared in step (4) at a rotation speed of 1000 r / min for 12 minutes to remove the sediment and take the clear liquid;

[0047] (6) Add 25 g of H with a mass concentration of 30% to the clear solution prepared in step (5) 2 O 2 , The culture medium is obtained...

Embodiment 3

[0050] A hatching medium for Artemia eggs, including the following ingredients by weight:

[0051]

[0052]

[0053] Wherein, the NaCl, KCl, MgSO 4 , SrCl 2 Chemically pure solid

[0054] The preparation method includes the following processing steps:

[0055] (1) Mix 25g of NaCl, 18gKCl, 38gMgSO 4 , 14gSrCl 2 Dissolved in 1000g of H 2 In O, stir evenly to obtain a mixed solution;

[0056] (2) Sterilize the mixture prepared in step (1) under ultraviolet light for 26 minutes;

[0057] (3) Add 0.2g glucose and 0.08g glucose oxidase to the sterilized mixed solution in sequence, and stir to dissolve;

[0058] (4) Add 2.5g of vitamin C sodium and 0.7g of tea polyphenols, and stir evenly at 50℃;

[0059] (5) Centrifuge the material obtained in step (4) at a speed of 1500r / min for 13 minutes, remove the sediment, and take the clear liquid;

[0060] (6) Add 26 g of H with a mass concentration of 30% to the clear solution prepared in step (5) 2 O 2 , The culture medium is obtained, and then the cul...

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Abstract

The invention relates to the field of production of aquaculture live fodder, and provides a hatching culture medium of artemia cysts hatched by fairy shrimps, and provides a preparation method and applications of the hatching culture medium of artemia cysts. The preparation method comprises the following processing steps: (1) dissolving 20-30 parts of NaCl, 15-20 parts of KCl, 30-40 parts of MgSO4, and 10-15 parts of SrCl2 in 1000 parts of H2O, uniformly stirring, to obtain a mixed liquor; (2) disinfecting; (3) adding 0.1-0.2 parts of glucose, 0.05-0.08 parts of glucose oxidase, stirring to dissolve; (4) adding vitamin C and tea polyphenol, uniformly stirring; (5) centrifuging and taking clear liquid; (6) adding H2O2 in the clear liquid, to obtain a culture medium, and rapidly transferring the culture medium in a closed transparent container.

Description

Technical field [0001] The invention relates to the production field of aquaculture live feed, in particular to a hatching culture medium for Artemia eggs and a preparation method and application. Background technique [0002] Artemia nauplii is generally used as live feed for aquaculture, especially as live feed for marine fish and shrimp in the early stage of larvae. There is generally no live feed like Artemia nauplii on the market, only Artemia larvae are hatched from Artemia larvae. The hatching of Artemia eggs can be done in the hatching medium. Artemia eggs can survive for a long time or even several years after being fully dehydrated and in a dry environment with low temperature, oxygen and no light. Long storage capacity and short time (within 24 hours) can produce free-swimming nauplii characteristics, making it the most convenient source of live feed for aquaculture. Artemia eggs are collected from nature such as seaside salt pans and inland salt lakes. Among the c...

Claims

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Application Information

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IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 陈乌雄李秀铿
Owner XIAMEN JINYIHAI BIOTECH CO LTD
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