Anti-gonyautoxin GTX2,3 monoclonal antibody immuno-colloidal gold labeled test strip and preparation method thereof
A technology of gonitoxin and monoclonal antibody, which is applied in biological testing, material inspection products, measuring devices, etc., can solve the problems of difficult preparation of artificial antigens and difficult realization of monoclonal antibody gold standard test strips, etc., and achieve a good society And economic value, easy to promote and apply, good stability
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Embodiment 1
[0024] An immunocolloidal gold-labeled test strip of anti-globulina toxin GTX2, 3 monoclonal antibody, the bottom of the test strip is a support layer, the middle layer is an adsorption layer, the protective layer is fixed on the adsorption layer, and the adsorption layer is from The test end is the adsorption fiber layer, the nitrocellulose membrane layer and the water-absorbing material layer at the end in sequence. The gold-labeled antibody in the gold-labeled antibody fiber layer is an anti-GTX toxin monoclonal antibody; The toxin has a control line (C) and a test line (T).
[0025] A method for preparing an immunocolloidal gold-labeled test strip of anti-gonitoxin GTX2,3 monoclonal antibody, including the coating of antigen GTX2,3-Gly-KLH, Preparation, colloidal gold preparation, colloidal gold-labeled antibody, test paper preparation steps:
[0026] Preparation of monoclonal antibody to GTX2,3: the hybridoma cell line 4C2 was screened and cultivated in the splenocytes o...
Embodiment 2
[0032] An immunocolloidal gold-labeled test strip of anti-globulina toxin GTX2, 3 monoclonal antibody, the bottom of the test strip is a support layer, the middle layer is an adsorption layer, the protective layer is fixed on the adsorption layer, and the adsorption layer is from The test end is the adsorption fiber layer, the nitrocellulose membrane layer and the water-absorbing material layer at the end in sequence. The gold-labeled antibody in the gold-labeled antibody fiber layer is an anti-GTX toxin monoclonal antibody; The toxin has a control line (C) and a test line (T).
[0033] A method for preparing an immunocolloidal gold-labeled test strip of anti-gonitoxin GTX2,3 monoclonal antibody, including the coating of antigen GTX2,3-Gly-KLH, Preparation, colloidal gold preparation, colloidal gold-labeled antibody, test paper preparation steps:
[0034] Preparation of monoclonal antibody to GTX2,3: the hybridoma cell line 4C2 was screened and cultivated in the splenocytes o...
Embodiment 3
[0040] An immunocolloidal gold-labeled test strip of anti-globulina toxin GTX2, 3 monoclonal antibody, the bottom of the test strip is a support layer, the middle layer is an adsorption layer, the protective layer is fixed on the adsorption layer, and the adsorption layer is from The test end is the adsorption fiber layer, the nitrocellulose membrane layer and the water-absorbing material layer at the end in sequence. The gold-labeled antibody in the gold-labeled antibody fiber layer is an anti-GTX toxin monoclonal antibody; The toxin has a control line (C) and a test line (T).
[0041] A method for preparing an immunocolloidal gold-labeled test strip of anti-gonitoxin GTX2,3 monoclonal antibody, including the coating of antigen GTX2,3-Gly-KLH, Preparation, colloidal gold preparation, colloidal gold-labeled antibody, test paper preparation steps:
[0042] Preparation of monoclonal antibody to GTX2,3: the hybridoma cell line 4C2 was screened and cultivated in the splenocytes o...
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