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High-efficiency microflow electrochromatography similarity analysis method and influence degree calculating method

A technology of microcurrent chromatography and similarity analysis, which is applied in the field of high-efficiency microcurrent chromatography similarity analysis and calculation of influence, and can solve the problems of lack of high-reliability calculation methods

Active Publication Date: 2016-04-27
上海市计算技术研究所
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  • Application Information

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Problems solved by technology

[0005] However, the current existing technology has not yet combined the alignment steps, similarity calculation, influence calculation, etc. to form a complete technical solution, and the calculation method of influence is relatively simple and lacks a high-reliability calculation method

Method used

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  • High-efficiency microflow electrochromatography similarity analysis method and influence degree calculating method
  • High-efficiency microflow electrochromatography similarity analysis method and influence degree calculating method
  • High-efficiency microflow electrochromatography similarity analysis method and influence degree calculating method

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Embodiment

[0036] The experimental environment of this example is: mobile phase: 0.02mol / L sodium dihydrogen phosphate diethylamine buffer solution + methanol (70:30); flow rate: 1mL / min; detection wavelength: 220nm; injection volume: 50μL; Temperature: 30°C; Chromatographic column: C18 (l50mm×4.6mm, particle size 5μm).

[0037] The concrete steps of this embodiment are:

[0038] 1. Select the data list from the data source; give the data source and the specific list. Similarly, specific values ​​must be given for each step below.

[0039] 2. Select the peak to be aligned, the initial value is the last peak, and only one peak can be selected in each row;

[0040] 3. Based on the first data, perform the alignment operation:

[0041] T 1 : the response time of the aligned peaks in the first row;

[0042] T 2 : the response time of the current row aligned peak;

[0043] T x : the response time of the peak before alignment;

[0044] T y : the response time of the peak after alignme...

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Abstract

The invention relates to a high-efficiency microflow electrochromatography similarity analysis method and an influence degree calculating method. The method comprises the following steps: aligning peaks on different atlas by an aligning step, and calculating the peaks by a similarity equation to obtain a similarity value of each detection substance in relative chromatogram, according to the value, determining that whether a substance to-be-detected and a standard sample belong the same substance or not. And finally, calculating influence degree of peaks in similarity calculation by using an influence degree equation. According to the method, whether the substance to-be-detected and the standard sample belong the same substance or not can be effectively compared, and the influence degree of peaks the same substance to be detected in similarity calculation can be obtained, so that high-efficiency microflow electrochromatography analysis and sample identification capability can be increased.

Description

technical field [0001] The invention relates to the field of high-efficiency micro-current chromatography sample analysis, in particular to a method for analyzing the similarity of high-efficiency micro-current chromatography and calculating the degree of influence. Background technique [0002] High-efficiency microcurrent chromatography is a very important separation and analysis instrument. Its greatest role is to determine the name of the sample to be tested and analyze the composition of the sample to be tested. [0003] In order to enable high-efficiency microcurrent chromatography to accurately analyze the name of the sample to be tested, the current field of chromatographic analysis often introduces the concept of similarity into the calculation module, such as Chinese patent CN104007212. Due to the uncertainty of the instrument, the sample to be tested, the chromatographic column and the solvent, before performing the similarity calculation, it is often necessary to...

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Application Information

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IPC IPC(8): G01N30/86
Inventor 张骏之徐圣翔王强荣
Owner 上海市计算技术研究所
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