XL-2 human lung cancer highly metastatic cell line and applications

Abstract

The invention belongs to the field of cytobiology, and discloses a human lung cancer XL-2 highly metastatic cell line, called XL-2sci for short. The human lung cancer XL-2 highly metastatic cell line is characterized by being prepared through the step that conventional lung cancer cells XL-2 cells are subjected to animal in-vivo cyclic screening, and the conventional lung cancer cells XL-2 cells are preserved in the China Center for Type Culture Collection (CCTCC), and have the preservation number CCTCC NO C201282. The human lung cancer XL-2 highly metastatic cell line has the advantages that (1) through the repeated in-vivo screening, a human lung cancer XL-2 highly metastatic cell line model and a human lung cancer XL-2 highly metastatic cell line are successfully established, the metastasis rate is high, the metastasis is stable, the intuition is good, and the targeting is strong; (2) with the human lung cancer XL-2 highly metastatic cell line provided by the invention, an ideal model is provided for the prevention and cure research and anti-metastasis treatment on lung cancer.

Description

technical field [0001] The invention relates to the field of cell biology, in particular to a human lung cancer highly metastatic cell line and its application. Background technique [0002] Tumor metastasis is a multi-stage, multi-step, multi-factor, and multi-gene interaction process that is extremely complex. From the detachment of cancer cells from the tumor mother to the formation of metastases, complex processes and mechanisms such as adhesion and de-adhesion, proteolysis, and motility have to go through. In patients with malignant tumors, there may be many tumor cells that enter lymphatic vessels or blood vessels, but only certain subgroups of tumor cells grow preferentially and acquire a metastatic phenotype, and these tumor cells with high metastatic potential are prone to metastasize. Animal experiments also proved that transplanting T241 sarcoma cells into C 57 In BL / 6 mice, live tumor cells can be found in the blood within 24 hours, but metastases appear after t...

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Problems solved by technology

[0004] In addition, after continuous in vitro artificial culture and passage, many tumor cells can no longer maintain their original biological characteristics.

Therefore, the clinical predictive ability of the metastasis model established using this tumor cell is low

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