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Grain weight-associated protein originated from soybean and related biomaterial thereof, and application thereof

A technology of biological materials and recombinant microorganisms, applied to soybean-derived grain weight-related proteins and related biological materials and application fields

Active Publication Date: 2016-04-20
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past 50 years, soybean production has only increased by 62%, while other food crops have increased by more than 5 times. The production of soybeans lags far behind the pace of domestic production of other food crops, and can no longer meet the needs of the people. Therefore, the per unit yield of soybeans has been increased. has become an urgent problem to be solved

Method used

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  • Grain weight-associated protein originated from soybean and related biomaterial thereof, and application thereof
  • Grain weight-associated protein originated from soybean and related biomaterial thereof, and application thereof
  • Grain weight-associated protein originated from soybean and related biomaterial thereof, and application thereof

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Experimental program
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Effect test

Embodiment 1

[0057] Embodiment 1, the cDNA gene preparation of protein GmHSFA2 related to soybean seed weight and the construction of plant expression vector

[0058] 1. Cloning of transcription factor GmHSFA2 gene and construction of plant expression vector

[0059] Total RNA was extracted from soybean Heinong 44 seedlings, and the RNA was reverse-transcribed with reverse transcriptase to synthesize cDNA. Using the cDNA as a template and GmHSFA2-up (5'-ATGACGACTCACGTAGTTAAAGA-3') and GmHSFA2-dp (5'-TCAACTGGTTTGTTCCACCAATC-3') as primers, PCR amplification was performed to obtain a PCR product of about 1.1 Kb. After sequencing, the PCR product is 1107bp, its nucleotide sequence is SEQIDNo.1, the DNA molecule shown in the nucleotide sequence is the cDNA gene (GmHSFA2) of GmHSFA2, and the protein encoded by the gene is named GmHSFA2, the amino acid of GmHSFA2 The sequence is SEQIDNo.2.

[0060] The Gateway system provided by invitrogen was used for gene cloning, and the 3′-T overhang of the ...

Embodiment 2

[0063] Embodiment 2, utilize GmHSFA2 to cultivate the transgenic seed plant of high grain weight

[0064] 1. Obtaining recombinant Agrobacterium

[0065] The recombinant vector pGWB405-GmHSFA2 containing GmHSFA2 obtained in Example 1 was introduced into Agrobacterium tumefaciens GV3101 by electroporation to obtain recombinant Agrobacterium GV3101 / GmHSFA2 containing pGWB405-GmHSFA2.

[0066] 2. Obtaining and identification of transgenic Arabidopsis thaliana

[0067] The recombinant Agrobacterium GV3101 / GmHSFA2 was cultured to the logarithmic phase, and then transformed into Arabidopsis thaliana (Col-0) (seeds were purchased from Arabidopsis Biological Resource Center, ABRC) by vacuum method to obtain T 0 Substitution of GmHSFA2 Arabidopsis. Seeds harvested after cultivation (T 1 generation), the seeds were sown on the MS screening medium containing kanamycin (50mg / L), and the T 1 When the generation plant grows to 4-6 leaves, it is moved to the vermiculite to grow, and the ...

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Abstract

The invention discloses a grain weight-associated protein originated from soybean and a related biomaterial thereof, and application thereof. The protein provided by the invention is named as GmHSFA2 and is a protein as described in a) or b), wherein a) is a protein with an amino acid sequence as shown in SEQ ID No. 2, and and b) is a protein derived from a) through substitution and / or deletion and / or addition of one or more amino acid residues in the amino acid sequence represented by SEQ ID No. 2 in a sequence table and related to grain weight. Experimental results prove that GmHSFA2 and the biomaterial related to GmHSFA2 can be applied to regulating the grain weight of spermatophytes, especially to increasing of the grain weight of spermatophytes.

Description

technical field [0001] The invention relates to grain weight-related proteins derived from soybeans and related biological materials and applications in the field of biotechnology. Background technique [0002] Soybean is an important traditional crop, rich in nutrition, and an important economic crop that provides edible oil and vegetable protein. Soybean oil also has many uses in industrial production, such as biofuel, surfactant, softener, etc. At the same time, soybean is a kind of crop that is very friendly to the environment. It converts nitrogen in the atmosphere into the soil through rhizobia at an efficiency of 100 kilograms per hectare every year, so that plants can absorb it. It is suitable for crop rotation, continuous cropping and intercropping, etc. sustainable agricultural model. Although soybeans originated in China, domestic soybean production accounts for only one-third of demand, making China the world's largest soybean importer, accounting for half of t...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/84C12N1/21C12N5/10A01H5/10
Inventor 张劲松陈受宜李擎天张万科马彪林晴何锶洁
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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