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A kind of method for separating and purifying crocin and crocin from gardenia yellow pigment

A technology of gardenia yellow pigment and crocetin, which is applied in the field of separation and purification of crocetin and crocetin, can solve the problems of difficulty in realizing large-scale industrial production, complicated operation, and low purification efficiency, and achieve high separation and purification efficiency, The operation process is simple and the operation volume is large

Active Publication Date: 2017-10-31
WUHAN GREEN FOOD BIOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a method for separating crocin and crocetin from gardenia yellow pigment, which generally has complex operations, low purification efficiency, and difficulty in realizing industrialized large-scale production. Method for separating and purifying crocetin and crocetin in

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037]A method for separating and purifying crocetin and crocetin from gardenia yellow pigment, comprising the steps of:

[0038] (1) Rough separation of crocin from gardenia yellow;

[0039] Use gardenia yellow pigment with a color value of 180A as a raw material, dilute it into an aqueous solution of gardenia yellow with a color value of 5A, add citric acid to adjust the pH to 5.5, put it on an activated D-151 resin column, and control the flow rate of the outflow valve 3.5ml / min, collect the effluent liquid a; after the resin column is saturated and adsorbed, add 5BV of pure water to rinse the resin column;

[0040] The resin column is analyzed with an ethanol aqueous solution with a mass fraction of 70%, and the outflow rate of the analysis solution is controlled to be 2.0ml / min; 0.6-1.0A, collect this section of liquid b; the concentration of crocindin in the analysis solution in the middle stage of analysis is the highest, and the color value of the analysis solution is...

Embodiment 2

[0049] A method for separating and purifying crocetin and crocetin from gardenia yellow pigment, comprising the steps of:

[0050] (1) Rough separation of crocin from gardenia yellow;

[0051] Use gardenia yellow pigment with a color value of 170A as the raw material, dilute it into an aqueous solution of gardenia yellow with a color value of 5A, add citric acid to adjust the pH to 5, put it on an activated D-151 resin column, and control the flow rate of the outflow valve 3.0ml / min, collect the effluent a; after the resin column is saturated and adsorbed, add pure water 4BV to rinse the resin column;

[0052] Analyze the resin column with an ethanol aqueous solution with a mass fraction of 80%, and control the outflow rate of the analysis solution to be 1.5ml / min; collect the analysis solution in three sections. 0.6-1.0A, collect this section of liquid b; the concentration of crocindin in the analysis solution in the middle stage of analysis is the highest, and the color val...

Embodiment 3

[0060] A method for separating and purifying crocetin and crocetin from gardenia yellow pigment, comprising the steps of:

[0061] (1) Rough separation of crocin from gardenia yellow;

[0062] Use gardenia yellow pigment with a color value of 200A as a raw material, dilute it into an aqueous solution of gardenia yellow with a color value of 5A, add citric acid to adjust the pH to 6, put it on an activated D-151 resin column, and control the flow rate of the outflow valve 4.0ml / min, collect the effluent a; after the resin column is saturated and adsorbed, add 5BV of pure water to rinse the resin column;

[0063] The resin column is analyzed with a mass fraction of 70% ethanol aqueous solution, and the flow rate of the analysis solution is controlled to be 2.5ml / min; the analysis solution is collected in three sections, and the concentration of crocin in the initial analysis solution is relatively low, and the color value of the analysis solution is 0.6-1.0A, collect this secti...

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Abstract

The invention discloses a method for separation and purification of crocin and crocetion from gardenia yellow pigment. The method comprises the following steps: gardenia yellow pigment with the color value of 170-200A is taken as the raw material and diluted into a gardenia yellow water solution with the color value of 5A; adding acid to adjust the pH to be 5-6; a D-151 resin column is adopted to extract crocin from the gardenia yellow pigment; a DEAE-cellulose adsorption column is adopted to refine the crude extracted crocin to prepare crocin powder with the purity of 79-82%, wherein the yield of crocin is 36.8-40%; after adding ethanol into a residual liquid of the crude extracted crocin and the refined crocin, adding ethyl acetate for extraction so as to prepare crocetion with the purity of 69.5-75%, wherein the yield of crocetion is 20-26%. The method provided by the invention starts from the raw material of gardenia yellow pigment, is simple in operation process, high in separation and purification efficiency and large in operation volume, and can realize industrial large-scale production.

Description

technical field [0001] The invention belongs to the technical field of deep processing of gardenia yellow pigment, in particular to a method for separating and purifying crocin and crocetin from gardenia yellow pigment. Background technique [0002] Gardenia yellow pigment is a kind of natural carotene series pigment extracted from gardenia fruit, and its main components contain crocetin and crocetin. Crocetin is the product of the esterification reaction between crocetin and gentiobiose. It has good pharmacological effects, can resist oxidation, and has anticancer activity. It has good health care effect and high safety. It is used in medicine, food, chemical industry and other fields have a wide range of applications. Separation of crocetin and crocetin from natural gardenia yellow pigment has achieved large-scale production and has good application prospects and market potential. [0003] The Chinese patent application number is 201110417356.1, and the name is "a method...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H13/06C07H1/08C07C51/42C07C57/13
CPCC07C51/42C07H1/08C07H13/06C07C57/13
Inventor 梅元碧王力张伟
Owner WUHAN GREEN FOOD BIOLOGICAL ENG
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