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Thermal shock gasifying electrospray ionization source and mass spectrometry (MS) system

An electrospray mass spectrometry and electrospray technology, which is applied in the direction of mass spectrometer, ion source/gun, particle separator tube parts, etc., can solve the problem of low efficiency of applying heat energy to samples, coupling of chromatographic systems, and easy decomposition of sample molecules when heated To achieve the effect of improving application efficiency, high ionization efficiency, and shortening heating time

Active Publication Date: 2016-04-06
HEILONGJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current method of thermal desorption is inefficient in applying thermal energy to the sample, which leads to the easy decomposition of sample molecules when heated. It is only suitable for the analysis of small molecules that are thermally stable and volatile, and it is difficult to combine with a chromatographic system, thus limiting this method. application of technology

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  • Thermal shock gasifying electrospray ionization source and mass spectrometry (MS) system
  • Thermal shock gasifying electrospray ionization source and mass spectrometry (MS) system
  • Thermal shock gasifying electrospray ionization source and mass spectrometry (MS) system

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Embodiment 1

[0038] figure 1 As a specific embodiment of the thermal shock gasification electrospray ionization mass spectrometry system of the present invention, the mass spectrometry system includes two parts, that is, a mass spectrometer part and an ionization source part. Mass spectrometers are of a great variety and are widely used. The ionization source provided by the invention can be connected with any mass spectrometer with an atmospheric pressure interface, such as ion trap mass spectrometry, quadrupole mass spectrometry, time-of-flight mass spectrometry, Fourier transform ion cyclotron resonance mass spectrometry, Magnetic mass spectrometry, orbitrap mass spectrometry, etc. The mass spectrometer of the present invention includes a mass spectrometer inlet 4, through which the ions generated by the ionization source can be introduced into the mass analyzer for mass spectrometry analysis. The ionization source part is the thermal shock gasification electrospray ionization source ...

Embodiment 2

[0050] The thermal shock gasification electrospray ionization mass spectrometry system of the present invention is used to analyze thermally unstable compounds, and mesaconitine is used as the detection object. Add 100 μL of 10 mg / L aconitine methanol solution dropwise to the bottom of the unheated vaporization chamber, and at the same time turn on the electrospray, record the mass spectrometry data, and heat the bottom of the vaporization chamber to 320°C within 2 minutes to gradually vaporize it , but in addition to the background signal of the electrospray solvent in the collected mass spectrometry data (the spectrum is not listed), there is no signal related to mesoaconitine, indicating that mesoaconitine is completely decomposed during the heating process. Using the thermal shock gasification electrospray ionization method described in the present invention, first heat the bottom of the gasification chamber to 320°C, and then apply the methanol solution of aconitine to the...

Embodiment 3

[0052] The thermal shock gasification electrospray ionization mass spectrometry system of the present invention is used to analyze biomacromolecules, and horseheart cytochrome C (cytochromec) is used as the detection object. The electrospray solvent is a mixed solution of methanol / water (1:1) containing 0.1% formic acid, and 100 μL of 0.31 mg / ml cytochrome C aqueous solution is added dropwise to the bottom of the unheated vaporization chamber. At the same time, the electrospray is turned on, and the mass spectrometry data is recorded. Afterwards, the vaporization chamber was heated to 400°C within 2 minutes to gradually vaporize it. However, the collected mass spectrometry data did not show any correlation with cytochrome C except for the background signal of the electrospray solvent (the spectrogram is not listed). The signal indicated that cytochrome C was denatured during the heating process and did not enter the mass spectrometry system.

[0053] Using the thermal shock ga...

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Abstract

The invention discloses a thermal shock gasifying electrospray ionization source and a mass spectrometry (MS) system, belonging to the technical field of ambient MS ionization. The MS ionization source disclosed by the invention comprises an electrospray generator, a heating device and a sampling device, wherein the heating device comprises a gasifying chamber and a heater providing heat for the gasifying chamber; a sample nozzle of a sample tube of the sampling device is configured in a way of facing the bottom of the gasifying chamber; the outlet end of a spraying capillary tube of the electrospray generator is opposite to the inlet of a mass spectrometer and is spaced from the inlet by a certain distance; the gasifying chamber and the sample nozzle are placed under the space between the outlet end of the spraying capillary tube and the inlet of the mass spectrometer. The ionization source provided by the invention gasifies a sample in a thermal shock manner and improves the heating efficiency of the sample through shortening heating time; since the sample is heated for a short time, heat energy is not converted into the molecular internal energy of the sample and only can be provided for the sample to overcome the intermolecular force, and the molecules of the sample cannot be decomposed due to heating; thus, the ionization source provided by the invention can be used for detecting heat-unstable and nonvolatile compounds and biological samples and solves the difficulty existing in the traditional thermal desorption technology.

Description

technical field [0001] The invention relates to a thermal shock gasification electrospray ionization source and a mass spectrometry analysis system, belonging to the technical field of atmospheric mass spectrometry ionization. Background technique [0002] The ratio (mass-to-charge ratio) of the charge of the analyte molecule to its mass (mass-to-charge ratio) during the analysis process can be ascertained by mass spectrometry (MS) analysis technology, so that the molecular weight information of the molecule or its fragments can be obtained. As an important analytical technique, mass spectrometry has the advantages of high sensitivity, high throughput, and good quantitative linearity. The first mass spectrometer was made in 1919. Since then, mass spectrometry technology has developed rapidly in the field of analytical chemistry, and has become a major instrument in chemistry, medicine, materials, biology, medicine, environment, energy, food, criminal forensics, etc. Common ...

Claims

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Application Information

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IPC IPC(8): H01J49/10H01J49/26
CPCH01J49/10H01J49/26
Inventor 许庆轩
Owner HEILONGJIANG UNIV
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