Preparation method of corn processing by-product fermented feed and used composite microbial agent
A technology of compound bacterial agent and fermented feed, applied in the direction of microorganism-based methods, biochemical equipment and methods, animal feed, etc., can solve the problems of high dependence on imports, lack of protein and energy feed resources, and increased cost of livestock and poultry breeding, etc. problems, to achieve the effect of increasing protein resources, good shape, and good palatability
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Embodiment 1
[0063] Embodiment 1, the preparation of composite bacterial agent
[0064] The compound microbial agent for preparing fermented feed, its active ingredient is composed of Bacillus subtilis GCMCC1.0892, Saccharomyces cerevisiae CGMCC2.1190 and Lactobacillus acidophilus CGMCC1.1854, which is Bacillus subtilis The mixture obtained by mixing the liquid strain of Saccharomyces cerevisiae and the liquid strain of Lactobacillus acidophilus.
[0065] The liquid strain of Bacillus subtilis is a culture obtained by culturing at 34-35°C for 18-24h with nutrient broth medium, and the content of Bacillus subtilis in the liquid strain of Bacillus subtilis is 1.3×10 8 cfu / mL;
[0066] The liquid strain of Saccharomyces cerevisiae is a culture obtained by culturing wort medium at 31-33°C for 18-24h, and the content of Saccharomyces cerevisiae in the liquid strain of Saccharomyces cerevisiae is 4.8×10 8 cfu / mL;
[0067] The liquid strain of Lactobacillus acidophilus is a culture obtained by...
Embodiment 2
[0069] Embodiment 2, the preparation of fermented feed 1 and its functional verification
[0070] One, the preparation of fermented feed 1
[0071] The components and their mass ratios in the fermentation raw materials are as follows: 38 parts of corn fiber feed: 10 parts of corn dregs: 40 parts of corn flour: 5 parts of corn bran: 5 parts of rice bran: 2 parts of molasses. Add water to the fermentation raw material according to the mass ratio of 1.2:1 according to the ratio of material to water to obtain the fermentation medium, and sterilize it at 121° C. for 20 minutes. Add it into the fermentation medium, and ferment for 48 hours at a fermentation temperature of 35° C. to obtain a fermented feed 1 . The product quality is shown in Table 1 and Table 2: fermented feed 1 is rich in probiotics Bacillus subtilis, Saccharomyces cerevisiae and Lactobacillus acidophilus; crude protein content, polypeptide content, amylase activity and protease activity in fermented feed 1 , cell...
Embodiment 3
[0089] Embodiment 3, the preparation of fermented feed 2 and its functional verification
[0090] One, the preparation of fermented feed 2
[0091]The components and their mass ratios in the fermentation raw materials are as follows: 20 parts of corn fiber feed: 30 parts of corn dregs: 20 parts of corn flour: 10 parts of corn bran: 7 parts of rice bran: 3 parts of molasses. Add water to the fermentation raw material according to the mass ratio of 1.2:1 according to the ratio of material to water to obtain the fermentation medium, and sterilize it at 121° C. for 20 minutes. Add it into the fermentation medium, and ferment for 48 hours at a fermentation temperature of 35° C. to obtain a fermented feed 2. The product quality is shown in Table 5 and Table 6: fermented feed 1 is rich in probiotics Bacillus subtilis, Saccharomyces cerevisiae and Lactobacillus acidophilus; crude protein content, polypeptide content, amylase activity and protease activity in fermented feed 2 , cellu...
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