Scanning Ion Conductance Microscope Glass Probe Holder and Illumination Device

Abstract

The invention provides a scanning ion conductance microscopy (SICM) glass probe clamping and illuminating device. The device mainly comprises a clamping module used for fixing a glass microprobe and a light source system used for illumination during SICM scanning measurement. The clamping module is mainly constituted by a device substrate, a sealing ring disposed in the cavity disposed in the bottom part of the substrate, a pipe cap fixedly connected with the lower end of the substrate by threads, an electric plug seat fixedly connected with the left side hole of the upper end of the pedestal by the threads, a sealing ring, an inserting pin fixedly disposed in the plug seat, and an Ag / AgC1 wire electrode connected with the inserting pin by adopting the welding connecting technology. The light source system is constituted by a lens fixedly disposed on the top end of the substrate, a battery seat fixedly connected with the top end of the substrate by the threads, a high light LED fixedly disposed on the bottom part of the battery seat, a button battery disposed in the battery seat, and a switch fixedly disposed on the top part of the battery seat. The device provided by the invention is advantageous in that the structure is simple and compact, the integration degree is high; the reliable clamping of the glass microprobe can be realized, and the device can be used for the illumination during the SICM scanning measuring process, and then the condition of the tip end of the probe entering the working area can be observed; the optimizing of the structure of the scanning ion conductance microscopy can be facilitated, and the efficiency of the glass probe entering the working area can be increased.

Description

technical field [0001] The invention relates to a glass probe clamping and illuminating device of a scanning measuring instrument SICM for the surface topography of living cells, and belongs to the technical field of scanning probe microscopy. Background technique [0002] Scanning ion conductance microscopy monitors the ionic current passing through the glass probe in real time, and negative feedback controls the distance between the probe tip and the sample surface, so as to achieve real-time, continuous, non-contact, high-resolution imaging of the surface microstructure of living cells. It has a very broad application prospect in the field of cell detection and soft interface research. Currently, scanning ion conductance microscopes use patch clamp electrode holders to fix glass microprobes. Different from the patch clamp measurement, the scanning ion conductance microscope probe and the inverted microscope stage are perpendicular to each other, so that the top light sou...

AI Technical Summary

Problems solved by technology

Different from the patch clamp measurement, the scanning ion conductance microscope probe and the inverted microscope stage are perpendicular to each other, so that the top light source of the inverted microscope cannot irradiate the light to the probe tip through the existing probe holder, and an external light source must be used To increase the brightness of the field of view of an inverted microscope to observe the entry of glass microprobe tips into the workspace

However, in order to improve the anti-interference ability of the scanning ion conductance microscope measurement system, the three-dimensional scanning head of the scanning ion conductance microscope is usually placed in a shielded box, and the narrow space makes it difficult to place a light source inside it. Open the shielding box door and irradiate the probe tip obliquely to observe the probe tip entering the working area

This reduces the shielding effect of the cabinet to a certain extent, and also affects the performance of the scanning ion conductance microscope

In addition, the electrical socket and the base of the existing glass probe holder are fixedly connected together, cannot be disassembled, and are expensive

When the Ag / AgCl electrode wire is broken, it must be replaced with a new glass probe holder

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