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Perinatal period pregnant woman group B streptococcus sampling tubes and preparation method thereof

A technology for sampling tubes and Streptococcus lactis, which is applied to biological material sampling methods, biochemical equipment and methods, biochemical instruments, etc., can solve the problems of low positive rate, achieve remarkable results, shorten the time of bacteria enrichment, and have wide application prospects Effect

Inactive Publication Date: 2016-03-30
DONGGUAN TUNGWAH HOSPITAL +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The positive rate of this method is very low, often around 1%.

Method used

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  • Perinatal period pregnant woman group B streptococcus sampling tubes and preparation method thereof

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Embodiment 1

[0020] The present invention provides a Streptococcus agalactiae sampling tube prepared according to the operation method for collecting and processing samples of Group B Streptococcus in peripartum pregnant women, including a test tube containing a nutrient solution, and the nutrient solution includes T-H Broth, agar powder and gentamicin, the dosage ratio of the T-H broth: agar powder: gentamicin is 250mL: 1g: 40mg.

[0021] The preparation method of the Streptococcus agalactiae sampling tube mentioned above comprises the following steps: add 1g of agar powder to 250mL of T-H broth, boil and let cool to 45-55°C, add 40mg of gentamycin, mix well and distribute to the In the test tubes of cotton swabs, each tube is 2.5-3.5 mL, and the Streptococcus agalactiae sampling tubes are obtained and stored in the refrigerator for the collection of vaginal secretions and rectal swabs of perinatal pregnant women.

[0022] The above-mentioned Streptococcus agalactiae sampling tube is used...

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Abstract

The invention discloses an operating method for collecting and treating specimens of perinatal period pregnant woman group B streptococcus and a preparation method of streptococcus agalactiae sampling tubes, and belongs to the technical field of sampling. The preparation method of the streptococcus agalactiae sampling tubes comprises: adding 1g of agar powder into 250mL of T-H broth, boiling, and cooling to 45-55 DEG C; and adding 40mg of gentamicin, mixing uniformly, and then sub-packaging the mixture into test tubes provided with cotton swabs, 2.5-3.5mL for each tube, thereby obtaining streptococcus agalactiae sampling tubes which are stored in a refrigerator. The method for collecting and treating the specimens of the perinatal period pregnant woman group B streptococcus is significant in effect of detecting 925 strains of streptococcus agalactiae from 10141 perinatal period pregnant women. The streptococcus agalactiae sampling tubes are designed on the basis of the operating method for collecting and treating the specimens of the perinatal period pregnant woman group B streptococcus, can shorten one day in comparison with the operating method for collecting and treating the specimens of the perinatal period pregnant woman group B streptococcus, and can achieve a same separation effect.

Description

technical field [0001] The invention belongs to the technical field of sampling, in particular to a sampling tube for group B streptococcus in perinatal pregnant women and a preparation method thereof. Background technique [0002] Streptococcus agalactiae, also known as Group B streptococcus (GBS), is an opportunistic pathogen that parasitizes the lower digestive tract and genitourinary tract of the human body. Its infection can induce a variety of maternal diseases, and the infection of pregnant women is manifested as bloodstream infection. , urinary system infection, fetal membrane infection, endometrial infection and trauma infection; it can also cause neonatal pneumonia, meningitis and other diseases. Monitoring the status of group B streptococcal infection in perinatal pregnant women is of great significance for improving the prevention and treatment of group B streptococcal infection and ensuring the health of mother and child. Because there is no requirement for the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/24C12Q1/14
CPCC12M23/08C12M33/02C12Q1/14
Inventor 张丽华张丽
Owner DONGGUAN TUNGWAH HOSPITAL
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