TERC gene and/or MYC gene detection probe, preparation method thereof and reagent kit

A gene detection and kit technology, which is applied in the field of TERC gene and/or MYC gene detection probes and their preparation, can solve the problems of lack of sensitivity and specificity detection kits, etc., and achieve accurate signal counting and good repeatability Effect

Inactive Publication Date: 2016-03-23
GUANGZHOU LBP MEDICINE SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, for TERC gene and / or MYC gene FISH detection, there is still a lack of detection kits with high sensitivity and specificity

Method used

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  • TERC gene and/or MYC gene detection probe, preparation method thereof and reagent kit
  • TERC gene and/or MYC gene detection probe, preparation method thereof and reagent kit
  • TERC gene and/or MYC gene detection probe, preparation method thereof and reagent kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The preparation of embodiment 1TERC gene and MYC gene detection probe

[0038] The preparation method of the described TERC detection probe of this implementation comprises the following steps:

[0039] Select the clones containing the target gene TERC and the sequences at both ends, such as Figure 1A shown. GSPTERC includes two sets of probes, the first set of probes includes the first probe, the second probe and the third probe, as shown in the table below, which were purchased from Invitrogen RP11BAC and CTDBAC clone libraries.

[0040] The second group of probes includes the fourth probe, the fifth probe and the sixth probe, as shown in the following table, which were purchased from Invitrogen RP11BAC and CTDBAC clone libraries.

[0041] The following two groups of detection probes were prepared respectively.

[0042] First group:

[0043] group one

BAC

Insert segment start and end position

first probe

CTD-3214J12

(chr3:169152582....

Embodiment 2

[0064] Embodiment 2: TERC gene and MYC gene detection kit preparation method

[0065] TERC gene and MYC gene detection kit include two components of TERC / MYC hybridization solution and DAPI counterstaining agent, wherein TERC and MYC hybridization solution comprise a group of GSPMYC gene probes and a group of GSPTERC gene probes described in Example 1 combination of needles. TERC and MYC gene respectively have two groups of detection probes, two groups of two kinds of genes can be combined at will, in the present embodiment, described TERC gene and MYC gene detection kit are four kinds, are respectively: TERC (group one)+ The combination of MYC (group 1), the combination of TERC (group 2) + MYC (group 2), the combination of TERC (group 1) + MYC (group 2), the combination of TERC (group 2) + MYC (group 1), CSP7 (identification probe for chromosome 7, purchased from D7Z1 SpectrumAquaProbe, catalog number: 06J54-007), buffer components for hybridization environment (to promote h...

Embodiment 3

[0073] Embodiment 3: the detection method of TERC gene and MYC gene detection kit

[0074] 1. Slide pretreatment

[0075] 1. Place the slides in a constant temperature oven at 65±5°C and bake overnight;

[0076] 2. Take out the slide and place it in xylene (or environmental dewaxing agent) at room temperature for 10 minutes;

[0077] 3. Take out the slide, and put it in another cylinder of room temperature xylene (or environmental dewaxing agent) for 10 minutes;

[0078] 4. Take out the slide, and put it in absolute ethanol at room temperature for 10 minutes to remove residual xylene (or environmental dewaxing agent);

[0079] 5. Take out the slide, and put it into 100%, 90%, 70% graded ethanol for rehydration at room temperature for 3 minutes each;

[0080] 6. Take out the slide, put it in sterilized purified water and wash it at room temperature for 3 minutes, and absorb excess water with a lint-free paper towel;

[0081] 7. Take out the slide, put the slide into 1×EDTA ...

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Abstract

The invention relates to a TERC gene and MYC gene detection probe and a preparation method thereof. The method comprises the following steps that at least one CTD-3214J12, RP11-816J6 and RP11-778I3 is selected as a BAC clone for a TERC gene, or at least one of RP11-3K16, CTD-2582E13 and RP11-886J24 is selected as a BAC clone; and / or CTD-2511O8 is selected as a BAC clone for an MYC gene, or at least one of RP11-440N18 and CTD-2205H22 is selected as the BAC clone; a plasmid DNA is obtained; labeling is performed. The invention further discloses a reagent kit containing the TERC gene and MYC gene detection probe. The optimal TERC gene and MYC gene detection probe is obtained through screening, signal counting row is accurate and rapid, and the result repeatability is good.

Description

technical field [0001] The invention belongs to biotechnology, and in particular relates to a TERC (ERBB2) gene and / or MYC gene detection probe, a preparation method and a kit. Background technique [0002] Cervical cancer is a common malignant tumor in gynecology. It is a gradual development process from normal cervix, cervical intraepithelial neoplasia to cervical cancer. Its occurrence and development are closely related to the abnormal expression or deletion of various genes and proteins, and it is a complex multi-step process. During the treatment of cervical cancer, it was found that the therapeutic effect of cervical cancer is related to the pathological grade. The therapeutic effect of CIN and early cervical cancer (including carcinoma in situ and early invasive carcinoma) is very good, and the treatment measures can be long-term tumor-free Survive. Therefore, early diagnosis and accurate grading of cervical cancer are very important in the prevention and treatment...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11C12N15/10
CPCC12Q1/6811C12Q1/6841C12Q1/6886C12Q2563/107C12N15/10C12N15/11C12Q1/68
Inventor 何瑰陈绍宇席影张会清
Owner GUANGZHOU LBP MEDICINE SCI & TECH
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