Molecular marking method for major QTL locus qRR5 for rooting rate in cuttage of catalpa bungei

A molecular marker and rooting rate technology, applied in the field of molecular genetics, to achieve the effects of accelerating promotion, shortening the breeding cycle, and reducing breeding costs

Inactive Publication Date: 2016-03-02
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Molecular marking method for major QTL locus qRR5 for rooting rate in cuttage of catalpa bungei

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[0008] Statistical analysis of the rooting rate of Catalpa cuttings: In early March 2013 and early March 2014, 87 five-year-old branches of Catalpa clones were cut into 30 cm long and placed evenly in a flat sand bed, and then covered with 3 ~5cm of fine sand, pour water thoroughly. Spray and irrigate the sand bed with 500 times of carbendazim turbid solution every week. Mix peat and perlite at a volume ratio of 1:1, put them into seedling pots (15×15×20cm), and spray and water them with 500 times carbendazim. When the height of the newly germinated shoots in the sand bed reaches 10-15 cm, remove the tops of the shoots, and after a week, remove the shoots with the heels as cuttings. Cut off the leaves on the cuttings together with the petiole, leaving only the top leaf, and cut it in half. Dip the base of the treated cuttings in 75% alcohol to disinfect for 30 seconds, rinse them with clean water, then dip them in 3,000 mg / L IBA for 1 minute, insert the cuttings into the see...

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Abstract

The invention provides a molecular marking method for the major QTL locus qRR5 for the rooting rate in cuttage of catalpa bungei and belongs to the field of molecular genetics. 70 pairs of SSR molecule markers are used for determining gene types of 87 parts of clonal materials of catalpa bungei, and whole genome association linkage analysis is carried out with the combination of the rooting rate in cuttage of catalpa bungei. The major QTL locus qRR5 for the rooting rate in cuttage of catalpa bungei is detected, 13.35% of phenotypic variation is explained, and the SSR molecular marker CB252 is highly remarkably associated with phenotypic variation. The molecular marking method is beneficial to solving the problem of the slow progress of catalpa bungei breeding in China and beneficial to solving the technical problem that the existing breeding technology is high in cost, long in time, poor in stability and the like when applied to increasing the rooting rate in cuttage of catalpa bungei, and breeding of new varieties of catalpa bungei in China and clone forestry development can be accelerated.

Description

technical field [0001] The invention provides a molecular marker method for the main effect QTL site qRR5 of the rooting rate of catalpa cuttings, belongs to the field of molecular genetics, and is specially used for directional cultivation of new species of catalpa cuttings that are easy to root. Background technique [0002] Catalpa ( Catalpa bungei ) belongs to Bignoniaceae ( Bignoniaceae ) Catalpa ( Catalpa ) is an excellent and precious timber tree species. With the development of industry and the growth of the world population, the demand for wood is increasing day by day, and the shortage of wood has become a worldwide problem, especially in my country. The development of clonal forestry has become an effective way to alleviate the shortage of timber in my country, but the rooting rate of cuttings is the bottleneck restricting the development of clonal forestry. Catalpa is a tree species with low cutting rooting rate, and the difficulty of cutting rooting limits...

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6895C12Q2600/156
Inventor 王鹏杨如同李林芳李亚马玲玲王淑安汪庆
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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