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Arvc disease-specific human induced pluripotent stem cell strain and use thereof

A pluripotent stem cell and specific technology, applied in the field of human induced pluripotent stem cell lines, can solve the problems of side effects, complications, inability to achieve the same curative effect, etc., and achieve the effect of less side effects

Active Publication Date: 2020-03-17
SHENZHEN SANQI BIOTECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The therapeutic effect of drug therapy in the above treatment plan varies from person to person, often has heterogeneity, and generally has side effects, and for patients with arrhythmia, the same drug cannot achieve the same curative effect; non-drug therapy There are certain risks in the surgical process, and it is easy to cause some complications

Method used

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  • Arvc disease-specific human induced pluripotent stem cell strain and use thereof
  • Arvc disease-specific human induced pluripotent stem cell strain and use thereof
  • Arvc disease-specific human induced pluripotent stem cell strain and use thereof

Examples

Experimental program
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Embodiment Construction

[0058] The preferred embodiments of the present invention will be further described in detail below in conjunction with the accompanying drawings.

[0059] Material:

[0060] The disease sample used in this example is c.C631T in the PKP2 gene, thus causing Q211X. The father and son with family diseases were selected from the selection of disease samples, and their urine epithelial cells were obtained, and they were named ARVC1 urine Epithelial cells and ARVC2 urinary epithelial cells.

[0061] 1. Obtaining method

[0062] ARVC2 urinary epithelial cells were reprogrammed with Episome episome plasmids, and ARVC2 urinary epithelial cells were reprogrammed with retroviral pMXs-Sox2, Klf4, Oct4, and c-Myc (SKOM), as follows:

[0063] (1) Retroviral reprogramming

[0064] The reprogrammed donor urine epithelial cells were cultured in medium (CIB, Cat#Iso-1030) for expansion, 293T cells were used to coat the virus, and the urine epithelial cells were divided into 12-well plates by...

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Abstract

The invention discloses two ARVC (arrhythmogenic right ventricular cardiomyopathy) specific human induced multifunctional stem cell strains ARVC1 and ARVC 2 and application thereof. The ARVC specific human induced multifunctional stem cell strain ARVC1 is preserved in the China Center For Type Culture Collection, and a preservation number of the ARVC1 is CCTCC NO:C2015128. The two ARVC specific human induced multifunctional stem cell strains ARVC1 and ARVC 2 can be efficiently differentiated into cardiac muscle cells, a ratio of bouncing EB balls accounting for all EB balls is calculated to work out the directional differentiation efficiency which is more than 85%, representation of disease phenotypes is realized, and the ARVC specific human induced multifunctional stem cell strains ARVC1 and ARVC 2 can be subsequently used for ARVC mechanism research and medicine development.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to an ARVC disease-specific human induced pluripotent stem cell strain and its application. Background technique [0002] Arrhythmogenic cardiomyopathy (ACM) is a progressive hereditary cardiomyopathy and is the main cause of ventricular arrhythmia and sudden cardiac death (Sudden Cadiac Death, SCD) in people under the age of 35. ACM can involve one or both ventricles. Arrhythmogenic right ventricular cardiomyopathy (ARVC) is one of the typical subtypes of ACM. Both compartments may be involved. ARVC is morphologically similar to dilated cardiomyopathy, but its typical clinical manifestations are often arrhythmias rather than heart failure. Although early onset is characterized by cardiac arrhythmias, morphological changes and even heart failure develop as the disease progresses. In addition, the arrhythmia exhibited by ARVC is characterized by ventricular tachycardia with left bundle ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/10C12Q1/02A01K67/027A61K35/34A61P9/06C12R1/91
Inventor 刘文玲杨佳银李翠兰刘雨晴仇晓亮周敏李雷杨波
Owner SHENZHEN SANQI BIOTECH
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