Chemically-defined diet and artificial mass rearing method for wheat aphids
A kind of feed and technology for wheat aphid, which is applied in pure artificial feed and artificial large-scale breeding of wheat aphid, which can solve the problems of inability to give birth, short survival time, and low survival rate of adult aphids.
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Embodiment 1
[0045] Example 1. Screening of the pure artificial feed formula for the aphid aphid
[0046] 1. Design of pure artificial feed formula
[0047] On the basis of a large number of previous experiments, this experiment mainly investigated the ratio of amino acids in the pure artificial diet of Aphid arvensis. Select 25 kinds of nutrients and their initial concentrations shown in Table 1 according to the results of previous experiments, adopt the orthogonal design method, set three levels (concentration gradients) for the 25 kinds of nutrients, configure 54 kinds of feeds in total, and determine according to the test results Optimized pure artificial feed formula. The data obtained from the experiment were calculated with the corresponding mathematical formula (Wang Shi'an, 1995). The experimental data were tested by analysis of variance.
[0048] 2. Preparation of nutrient solution
[0049] The 54 feed formulas obtained in step 1 are formulated into nutrient solutions respect...
Embodiment 2
[0079] Embodiment 2, adopt raising method of the present invention to raise tube aphid
[0080] Randomly pick out 1,000 newly produced nymphs within 24 hours, use the aphid feeding device described in Example 1, and connect 20 nymphs in each aphid feeding device, a total of 50 aphid feeding devices. All the aphid feeders are placed on the net frame, the water container is placed under the net frame to keep moisture, and the yellow paper is covered on the top to lure the aphids to the artificial feeding bag.
[0081] During the growth and development stage from nymph to adult aphid, the best growth and development feed for Aphid aphid was used for feeding;
[0082] After the adult aphids enter the breeding stage, they are raised with the best breeding feed for the aphids;
[0083]Observe and record the molt number, death number, survival number and litter size of aphids every day, change the feed every three days, and observe and record for 30 days.
[0084] Experimental resu...
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