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Chemically-defined diet and artificial mass rearing method for wheat aphids

A kind of feed and technology for wheat aphid, which is applied in pure artificial feed and artificial large-scale breeding of wheat aphid, which can solve the problems of inability to give birth, short survival time, and low survival rate of adult aphids.

Inactive Publication Date: 2016-02-24
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The rearing results reported by Argandon et al. (1980,1982) showed that the early survival rate was high, but the survival time was short, and the survival rate of adult aphids was low or even unable to give birth.
There are very few reports on pure artificial diets that can successfully raise wheat aphids in large quantities

Method used

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  • Chemically-defined diet and artificial mass rearing method for wheat aphids
  • Chemically-defined diet and artificial mass rearing method for wheat aphids
  • Chemically-defined diet and artificial mass rearing method for wheat aphids

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1. Screening of the pure artificial feed formula for the aphid aphid

[0046] 1. Design of pure artificial feed formula

[0047] On the basis of a large number of previous experiments, this experiment mainly investigated the ratio of amino acids in the pure artificial diet of Aphid arvensis. Select 25 kinds of nutrients and their initial concentrations shown in Table 1 according to the results of previous experiments, adopt the orthogonal design method, set three levels (concentration gradients) for the 25 kinds of nutrients, configure 54 kinds of feeds in total, and determine according to the test results Optimized pure artificial feed formula. The data obtained from the experiment were calculated with the corresponding mathematical formula (Wang Shi'an, 1995). The experimental data were tested by analysis of variance.

[0048] 2. Preparation of nutrient solution

[0049] The 54 feed formulas obtained in step 1 are formulated into nutrient solutions respect...

Embodiment 2

[0079] Embodiment 2, adopt raising method of the present invention to raise tube aphid

[0080] Randomly pick out 1,000 newly produced nymphs within 24 hours, use the aphid feeding device described in Example 1, and connect 20 nymphs in each aphid feeding device, a total of 50 aphid feeding devices. All the aphid feeders are placed on the net frame, the water container is placed under the net frame to keep moisture, and the yellow paper is covered on the top to lure the aphids to the artificial feeding bag.

[0081] During the growth and development stage from nymph to adult aphid, the best growth and development feed for Aphid aphid was used for feeding;

[0082] After the adult aphids enter the breeding stage, they are raised with the best breeding feed for the aphids;

[0083]Observe and record the molt number, death number, survival number and litter size of aphids every day, change the feed every three days, and observe and record for 30 days.

[0084] Experimental resu...

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Abstract

The invention relates to a chemically-defined diet and artificial mass rearing method for wheat aphids. The chemically-defined diet for the wheat aphids comprises an artificial diet applicable to the growth and development of Sitobion avenae and an artificial diet applicable to the proliferation of Sitobion avenae, is an optimized formula of the two kinds of diets and can enable Sitobion avenae to survive normally, grow, develop and reproduce offspring, thereby having a broad application prospect. The rearing method for Sitobion avenae, provided by the invention, is characterized in that the growth and development diet is adopted at a young aphid stage of Sitobion avenae; and the proliferation and development diet is adopted at an adult aphid stage of Sitobion avenae. According to the chemically-defined diet and the artificial mass rearing method, the adult ratio and birth ratio of the aphids are increased, the mass rearing method is established, and a reliable and important evaluation means for providing test aphids with uniform nutrition background and implementing researches on aphid nutrition physiology, wheat insect-resisting substances and transgenic insect-resisting protein activity is provided.

Description

technical field [0001] The invention relates to the field of plant disease prevention and control, in particular to a whole pure artificial feed for wheat aphids and a method for artificial mass feeding. Background technique [0002] Wheat aphids (abbreviated as wheat aphids) are the main pests in wheat production in my country and even in the world, causing wheat yields to decrease by more than 10% all the year round, and more than 30% in major occurrence years; wheat aphids have seriously occurred at home and abroad in recent years. Wheat aphid is an important piercing-sucking pest on wheat. It mainly uses nymphs and adult aphids to suck the juice of wheat leaves, stems, and young ears, which affects the normal growth and development of wheat. White spikes are formed. The honeydew secreted by aphids on the leaves also affects the photosynthesis and respiration of plants, and also spreads barley yellow dwarf virus (BYDV), which seriously affects the yield and quality of wh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033A23K50/90A23K20/10
CPCA01K67/033
Inventor 陈巨莲范佳孙京瑞张莉倪汉祥
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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