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A method for improving the rooting rate of tissue cultured seedlings of the leaves

A technology for planting plantlets and tissue culture seedlings, applied in the field of plant tissue culture, can solve the problems of low rooting rate and difficulty in rooting tissue culture seedlings of plantlets, so as to improve the rooting rate, reduce the probability of seedlings carrying viruses, The effect of improving the survival rate

Active Publication Date: 2018-10-02
桂林亦元生现代生物技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The present invention aims at the current shortage of seedlings for the artificial cultivation of Abacus, establishes a rapid propagation system for tissue culture of Abacus, especially solves the problem of difficulty in rooting and low rooting rate of the tissue cultured seedlings of Abacus, and provides a basis for Part of the rapid propagation to provide technical assurance, suitable for large-scale production

Method used

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  • A method for improving the rooting rate of tissue cultured seedlings of the leaves
  • A method for improving the rooting rate of tissue cultured seedlings of the leaves

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Experimental program
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Effect test

Embodiment 1

[0040] A method for improving the rooting rate of tissue cultured plantlets of the leaves, comprising the following steps:

[0041] (1) Selection and aseptic pretreatment of explants: select disease-free and robust apical buds as explants, rinse with sterile water, put the material on the ultra-clean workbench, and use 75% alcohol Soak for 30s, then treat with 0.1% mercuric chloride solution for 8 minutes, wash with sterile water 4 times, and set aside.

[0042] (2) Induction culture: cut out the 0.2mm shoot tip of the treated explants and inoculate them on the surface of the induction medium. The composition of the induction medium is: the improved MS medium contains NAA0.5mg / L, BA1.0mg / L , sucrose 30g / L, agar 5g / L, germination began after 8 days of culture, and the initial growth was slow. After 30 days of induction culture, robust sterile seedlings were obtained, and the induction rate was 95%.

[0043] (3) Cut the sterilized seedlings in step (2) into long 1.0cm budded st...

Embodiment 2

[0048] A method for improving the rooting rate of tissue cultured plantlets of the leaves, comprising the following steps:

[0049] (1) Selection and aseptic pretreatment of explants: select disease-free and robust apical buds as explants, rinse with sterile water, put the material on the ultra-clean workbench, and use 75% alcohol Soak for 40s, then treat with 0.2% mercuric chloride solution for 6 minutes, wash with sterile water for 6 times, and set aside.

[0050] (2) Induction culture: cut out the 0.2mm budding stem section from the treated explants, and inoculate them on the surface of the induction medium. The composition of the induction medium is: the improved MS medium contains NAA 0.5mg / L, BA 0.5mg / L, sucrose 30g / L, agar 5g / L, germination began after 10 days of culture, and the initial growth was slow. After 30 days of induction culture, robust sterile seedlings were obtained, and the induction rate was 92%.

[0051] (3) in the step (2), the aseptic seedlings are cu...

Embodiment 3

[0056] A method for improving the rooting rate of tissue cultured plantlets of the leaves, comprising the following steps:

[0057] (1) Selection and aseptic pretreatment of explants: select disease-free and robust apical buds as explants, rinse with sterile water, put the material on the ultra-clean workbench, and use 70% alcohol Soak for 35s, then treat with 0.1% mercuric chloride solution for 14min, wash with sterile water 5 times, and set aside.

[0058] (2) Induction culture: cut out the 0.30mm shoot tip of the treated explant and inoculate it on the surface of the induction medium. The composition of the induction medium is: NAA0.4mg / L and BA0.9mg / L are contained in the improved MS medium , sucrose 32g / L, agar 5.5g / L, germination began after 8 days of culture, and the initial growth was slow. After 28 days of induction culture, robust sterile seedlings were obtained, and the induction rate was 93%.

[0059] (3) Cut the aseptic seedlings in step (2) into long 1.0cm bud s...

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Abstract

The invention relates to the technical field of plant tissue culture and especially relates to a method for improving a rooting rate of a Stemona tuberosa L. tissue culture seedling. The method comprises selecting a terminal bud of Stemona tuberosa L. as an explant, carrying out sterilization treatment on the explant, cutting a stem tip, transferring the stem tip into an induction medium, carrying out culture for 28-35 days to obtain an aseptic seedling, cutting the aseptic seedling to obtain 1.0-1.5cm sections with buds, inoculating a proliferation culture medium with the sections with buds, carrying out rosette bud induction culture for 18-25 days to obtain aseptic buds, cutting the aseptic buds to obtain stem sections with buds, inoculating a rooting medium with the stem sections with buds, carrying out seedling hardening on the rooting seedlings at the normal temperature in a dark place, and transplanting the seedlings into a turf or yellow soil base. The Stemona tuberosa L. tissue culture seedling has a rooting rate of 95%, a rooting seedling has a survival rate of 99%, rooting rate stability is good, the problems of high rooting difficulty and a low survival rate of Stemona tuberosa L. tissue culture seedling are effectively solved and a production cost is reduced.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for improving the rooting rate of tissue cultured seedlings of leaves. Background technique [0002] Stemona tuberosa Lour. is mainly produced in Guangxi, Hunan, Guizhou and other provinces, and is recorded in "Pharmacopia of the People's Republic of China" (2005, 2010 edition). It has the effects of returning to the lung meridian, slightly warm in nature, sweet and bitter, and has the effects of nourishing the lungs, lowering qi, relieving cough, killing insects and lice, etc. It is used for chronic cough, tuberculosis cough and pertussis; externally used for head lice, body lice, pinworms and Itching, etc. 140 domestic pharmaceutical companies have made granules, tablets, ointments, and syrups for internal and external use throughout the country. Many products have become well-known brands at home and abroad, and the production enterprises have also becom...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G22/60A01G24/28A01G24/10
CPCA01G22/00A01H4/008
Inventor 杨振徳苏钰琴杨杰花周传明莫燕兰盘飞兰玉舒中韦祥意罗文正蒋向军郭春晖
Owner 桂林亦元生现代生物技术有限公司
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