Pancreatic juice developer, and preparation method and application thereof

A chromogenic agent and pancreatic juice technology, applied in the field of medical testing, can solve problems such as low color sensitivity of pancreatic juice leakage, and insufficient color contrast, and achieve obvious color contrast, high color sensitivity, and accurate qualitative diagnosis Effect

Active Publication Date: 2016-02-17
JIANGSU PROVINCE HOSPITAL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The technical problem to be solved by the present invention is to provide a pancreatic juice chromogenic agent to solve the problems i

Method used

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  • Pancreatic juice developer, and preparation method and application thereof
  • Pancreatic juice developer, and preparation method and application thereof
  • Pancreatic juice developer, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Prepare the indicator: mix 1g of bromothymol blue sodium and 1g of phenol red sodium, and dilute to 2000mL with water to make a stock solution; dilute 10g of sodium bisulfate monohydrate to 100mL with water to make an additive solution. Add 55mL additive solution to 1000mL stock solution to make indicator;

[0069] Impregnation: Pour the prepared indicator into a clean container, immerse the medium-speed qualitative filter paper into the indicator, pick up the filter paper with tweezers after it is completely soaked, and put it in a dry and flat container, the indicator is evenly coated on the filter paper superior;

[0070] Drying: drying in an oven under normal pressure, the temperature is set at 68°C, and the time is 20 minutes;

[0071] High-temperature and high-pressure steam sterilization: put the dried test paper into a metal sterilization box, and perform high-temperature and high-pressure steam sterilization under the conditions of temperature 121.3°C, pressur...

Embodiment 2

[0074] Embodiment 2: water agent.

[0075] Prepare the indicator: mix 1g of bromothymol blue sodium and 1g of phenol red sodium, and dilute to 2000mL with water to make a stock solution; dilute 10g of sodium bisulfate monohydrate to 100mL with water to make an additive solution. Add 20mL additive solution to 1000mL stock solution to make indicator;

[0076] High-temperature and high-pressure steam sterilization: put the prepared indicator into a glass bottle, unscrew the cap and then sterilize with high-temperature and high-pressure steam. Tighten the bottle cap immediately after opening the high temperature and high pressure steam sterilizer;

[0077] Test: Add 1mL of the prepared indicator solution into the six-hole plate, pipette 5 microliters of pancreatic juice, serum, and water, respectively, and add them to the six-hole plate from left to right, and observe the color after the change is stable. Such as figure 2 As shown, the pancreatic juice is purple, and the serum...

Embodiment 3

[0078] Embodiment 3: Spray.

[0079] Prepare the indicator: mix 1g of bromothymol blue sodium and 1g of phenol red sodium, and dilute to 2000mL with water to make a stock solution; dilute 10g of sodium bisulfate monohydrate to 100mL with water to make an additive solution. Add 20mL additive solution to 1000mL stock solution to make indicator;

[0080] High-temperature and high-pressure steam sterilization: put the prepared indicator liquid into a glass bottle, unscrew the cap and then sterilize it with high-temperature and high-pressure steam, disassemble the plastic sprayer and put it into a metal sterilization box for sterilization, and sterilize the glass bottle and metal The sterilization conditions of the box are temperature 121.3°C, pressure 103.4kPa (1.05kg / cm2), and maintain for 20 minutes. After the sterilization is completed, open the high-temperature and high-pressure steam sterilizer and immediately tighten the bottle cap of the glass bottle to complete the instruc...

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PUM

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Abstract

The invention discloses a pancreatic juice developer, and a preparation method and an application thereof. The pancreatic juice developer comprises 0.03-0.70g/L of bromothymol blue sodium salt, 0.03-0.70g/L of phenol red sodium salt and 1.23-11.76g/L of sodium bisulfate monobydrate. Compared with pancreatic juice developers in the prior art, the pancreatic juice developer has the advantages of high chromogenic sensitivity and obvious color contrast in the intraoperative seepage detection process. The pancreatic juice developer can flexibly realize pancreatic juice visualization in the intraoperative and postoperative processes according to concrete demands by preparing different dosage forms and selecting carriers in order to accurately realize qualitative diagnosis and intraoperative positioning diagnosis of pancreatic fistula.

Description

technical field [0001] The invention belongs to the technical field of medical detection, and in particular relates to a pancreatic juice chromogen and its preparation method and application. Background technique [0002] Acid-base indicators are widely used in scientific research, industry, agriculture, chemistry, medicine and other fields, and can be used to display the acidity and alkalinity of liquids. Acid-base indicators are a class of organic weak acids or organic weak bases with complex structures, which can be partially ionized into indicator ions and hydrogen ions (or hydroxide ions) in solution, and due to structural changes, their Molecules and ions have different colors and thus appear different colors in solutions with different pHs. Acid-base indicators can be used chemically for the titration of liquids. [0003] There is a kind of mixed acid-base indicator, which mixes two acid-base indicators with complementary colors, which has the characteristics of nar...

Claims

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Application Information

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IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 宋旭军苗毅周泉刘祥瑞陆子鹏蒋奎荣唐建斌申有青
Owner JIANGSU PROVINCE HOSPITAL
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