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Poultry micro-ecological preparation as well as preparation method and application thereof

A technology for microecological preparations and poultry, which is applied in the field of avian microecological preparations and their preparation, can solve the problems of limiting the wide application of microecological preparations, affecting the use effect of products, instability of gastric acid and bile salts, etc., and achieves efficient prevention and control of avian Escherichia coli disease, enteric and water-soluble, and the effect of preventing and treating diarrheal diseases

Inactive Publication Date: 2016-02-17
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the production of most probiotics in China adopts extensive processes such as solid fermentation, and the products produced often coexist with target bacteria and polluted bacteria, which seriously affects the use effect of the products.
The microecological preparations on the market have problems such as low viable bacteria content, high water content, intolerant to antibiotics, and instability to gastric acid and bile salts, which affect their application effect on animals and limit the wide application of probiotics

Method used

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  • Poultry micro-ecological preparation as well as preparation method and application thereof
  • Poultry micro-ecological preparation as well as preparation method and application thereof
  • Poultry micro-ecological preparation as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] 1. Preparation of poultry probiotics

[0065] 1. Preparation of Lactobacillus salivarius Scgz-11 bacterial powder

[0066] Recover Lactobacillus salivarius Scgz-11, in MRS solid medium (composition (g / L): peptone 10.0, beef extract powder 8.0, yeast extract powder 4.0, glucose 20.0, dipotassium hydrogen phosphate 2.0, diammonium hydrogen citrate 2.0, Sodium acetate 5.0, magnesium sulfate 0.2, manganese sulfate 0.04, agar 14.0, Tween 801.0, pH value 6.5 ± 0.2) were subcultured twice, and then the bacterial strain was inoculated in MRS broth medium (component (g / L): peptone 10.0, beef extract powder 8.0, yeast extract powder 4.0, glucose 20.0, dipotassium hydrogen phosphate 2.0, diammonium hydrogen citrate 2.0, sodium acetate 5.0, magnesium sulfate 0.2, manganese sulfate 0.04, Tween 801.0, pH value 5.7±0.2) , 37°C, 150r / min, cultivated for about 15h to obtain the bacterial liquid. At this time, add sterilized protective agent (skimmed milk 20g / L, corn starch 20g / L, solu...

Embodiment 2

[0081] 5. The application of poultry microecological preparations in the prevention and treatment of poultry intestinal diseases

[0082] 1. Prevention and control effect of poultry probiotics on Campylobacter jejuni disease of poultry origin

[0083] Campylobacter jejuni animal experiment scheme, see Table 1

[0084] The 1-day-old SPF chickens were divided into 3 groups, 25 in each group. The pathogenic bacteria used in the animal experiment was avian-derived Campylobacterjejuni (Campylobacterjejuni) SCCJ008, which was isolated by the inventor from a chicken farm in Guangdong in 2012. The pathogenic strain and its pathogenicity were published in "China Animal Husbandry and Veterinary Medicine" in 2013. The title of the article is: Isolation and identification of Campylobacter jejuni from avian origin and preliminary research on its pathogenicity. Among them, the poultry probiotics group is referred to as the micro-preparation group for short. The poultry probiotics prepared...

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Abstract

The invention discloses a poultry micro-ecological preparation as well as a preparation method and application thereof. The poultry micro-ecological preparation is prepared from lactobacillus salivarius Scgz-11 bacterium powder, lactobacillus salivarius SCL87 bacterium powder and lactobacillus ingluviei SCGZ-9 bacterium powder; selected strains are strains separated from healthy chicken flocks; a plurality of times of inspection and screening of the strains prove that the strains are safe, reliable and stable. The poultry micro-ecological preparation has no pollution of infectious microbes in a production process, high viable count and long storage period, resists gastric acid and has enteric solubility and water solubility; the poultry micro-ecological preparation can be mixed with feed and water to feed and is convenient and feasible; the poultry micro-ecological preparation has the effects of efficiently preventing and controlling poultry campylobacter jejuni diseases, poultry salmonellosis or poultry colibacillosis; the poultry micro-ecological preparation also has the effects of improving the ecological balance of poultry intestinal flora, enhancing the immunologic function, promoting the growth, improving the feed utilization rate and preventing and controlling diarrhea diseases. The poultry micro-ecological preparation has very important meanings of guaranteeing food safety and human health.

Description

technical field [0001] The invention relates to a poultry microecological preparation and a preparation method thereof. The invention relates to a poultry microecological preparation and a preparation method and application thereof. Background technique [0002] Since the 20th century, drugs such as antibiotics, olaquindox, and quinolones have come out one after another, and the animal breeding industry has developed rapidly. These drugs have obvious effects in preventing and curing diseases and promoting animal growth. However, the irrational use or even abuse of these drugs has led to the emergence of a large number of drug-resistant strains, which greatly reduces or has no effect on the efficacy of the drugs. Food safety and human health. [0003] In order to solve the above problems, countries around the world, especially developed countries such as Europe, the United States, and Japan, have imposed strict restrictions on the types, methods of use, dosages, and compatib...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K10/18A23K50/75A61K35/747A61P31/04A61P1/12C12R1/225
Inventor 罗开健王媚张建民黄玉梅万红张宗尧聂荷敏刘哲
Owner SOUTH CHINA AGRI UNIV
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