Automatic nucleic acid extraction and pcr amplification microfluidic chip and its application method

Abstract

The invention provides a full-automatic nucleic acid extraction and PCR amplification micro-fluidic chip which comprises at least one main body, wherein the main body is distributed in equal distance around the chip; the main body comprises a nucleic acid extraction unit, a PCR amplification unit, a waste liquid unit and an exhaust unit, wherein the nucleic acid extraction unit is positioned on the main body and is used for extracting, washing and eluting nucleic acid in sequence under the driving of centrifugal force and capillary force; the PCR amplification unit is adopted for PCR reaction amplification; the waste liquid unit comprises a waste liquid cavity which is used for storing a waste liquid; the exhaust unit comprises exhaust holes and a plurality of exhaust channels; and the plurality of exhaust channels are communicated with the nucleic acid extraction unit, the PCR amplification unit and the waste liquid unit and are used for exhausting air. The invention discloses a micro-fluidic chip for achieving full-automatic nucleic acid extraction and PCR reaction through centrifugal force, capillary force and siphon phenomenon, the micro-fluidic chip has no integrated equipment such as pumps or valves, so that the manufacturing difficulty and cost of the chip are greatly reduced, and moreover the reliability of the chip is improved.

Description

technical field [0001] The present invention relates to the field of nucleic acid detection. More specifically, the present invention relates to a fully automatic nucleic acid extraction and PCR amplification microfluidic chip and a nucleic acid extraction and PCR amplification method. Background technique [0002] There have been many applications of fluidic chips in nucleic acid extraction and PCR amplification, but most applications are still in the laboratory stage. The reason is that in order to realize the processes of blood sample or tissue lysis, nucleic acid binding, cleaning, and elution, these nucleic acid extraction and PCR amplification microfluidic chips are integrated with many micropumps and microvalves, adding microfluidic chips. complexity, manufacturing difficulty, cost, and reduced reliability. At the same time, the equipment and devices required to drive these pumps and valves externally are also complicated, which limits the clinical application of th...

AI Technical Summary

Problems solved by technology

The reason is that in order to achieve blood sample or tissue lysis, nucleic acid binding, cleaning, elution and other processes, these nucleic acid extraction and PCR amplification microfluidic chips are integrated with many micropumps and microvalves, adding microfluidic chip The complexity, manufacturing difficulty, and cost reduce reliability

At the same time, the equipment and devices required to drive these pumps and valves externally are relatively complicated, which limits the clinical application of these chips.

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