Kit for SNP typing of obesity-prone genes of Chinese population and using method of kit

Abstract

The invention relates to a kit for SNP typing of obesity-prone genes of Chinese population and a using method of the kit, and particularly discloses a simultaneous qualitative typing kit aiming at twelve SNP sites of ten obesity-prone genes of the Chinese population and a using method of the simultaneous qualitative typing kit. Two sites of an FTO gene, one site of an MC4R gene, one site of an ADRB3 gene, one site of a PPAR gamma 2 gene, one site of an LEPR gene, two sites of an APOE gene, one site of a UCP 2 gene, one site of a GNPDA2 gene, one site of a KCTD15 gene and one site of an NEGR 1 gene are taken as detection objects, an amplification primer and an extension primer are designed for mutation of each SNP site, multiple PCR amplification and markup extension are performed, and genotypes of the twelve SNP sites are acquired simultaneously through capillary electrophoresis analysis. The kit is convenient to use, simple to operate, low in cost, high in flux, direct and reliable in detection result and suitable for large-scale screening of SNP typing of the obesity-prone genes of the Chinese population.

Description

technical field [0001] The invention relates to the field of SNP typing detection in the technical field of genetic engineering, in particular to a Chinese population obesity susceptibility gene SNP typing kit and a use method thereof. Background technique [0002] Obesity is a common nutritional metabolic disease. In 2002, WHO listed obesity as one of the global first-level risk factors causing the burden of human disease. Preventing the prevalence of obesity is also one of the biggest public health challenges faced by countries in the world in the first 50 years of the 21st century. Family analysis and twin studies have shown that genetic factors play a very important role in the occurrence of obesity. Some studies have shown that if one of the parents is obese, the probability of their children developing obesity is 41-50%, while if both parents are obese, the probability of obesity is 41-50%. The probability of obesity in children increases to 66-80%. Numerous studies ...

AI Technical Summary

Problems solved by technology

However, this method has disadvantages such as time-consuming, cumbersome operation, and low accuracy.

There are also PCR combined with DNA sequencing methods for the detection of gene polymorphism sites, but this method is limited in the application of large-scale population screening or detection of multiple genes and multiple sites

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