A kind of magnetic nanoparticle coated with water-soluble protein and its preparation method

A technology of magnetic nanoparticles and water-soluble proteins, which is applied in the preparation of in vivo test preparations, pharmaceutical formulations, X-ray contrast agents, etc., can solve the problems of uneven thickness of encapsulation agent, poor effect of nuclear magnetic resonance medical imaging, obstacles, etc. , to achieve the effects of easy access to the inside of tissue cells, excellent MRI medical signals, and common and easy-to-obtain reaction equipment

Inactive Publication Date: 2018-10-12
INST OF HIGH ENERGY PHYSICS CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the particles obtained by this method have the disadvantages of large size, low crystallinity, low saturation magnetization, poor medical imaging effect of nuclear magnetic resonance, and uneven thickness of the coating agent.
The other is iron oxide nanoparticles prepared by thermal decomposition under high temperature conditions. The size is relatively small and more uniform, and the magnetic properties are superior. However, serious defects such as poor water solubility and poor stability in physiological environments also hinder its in vivo Imaging Applications
However, nanoparticles with a large specific surface area tend to aggregate, leading to loss of magnetism, reduced surface activity, and rapid clearance from the body.

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  • A kind of magnetic nanoparticle coated with water-soluble protein and its preparation method
  • A kind of magnetic nanoparticle coated with water-soluble protein and its preparation method
  • A kind of magnetic nanoparticle coated with water-soluble protein and its preparation method

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preparation example Construction

[0026] The invention provides a method for preparing water-soluble protein-coated magnetic nanoparticles, comprising: mixing the protein with a magnetic metal salt, and the metal ions of the magnetic metal salt are complexed to the protein to obtain a protein-magnetic metal Intermediate dispersion product: adding alkali to the intermediate dispersion product of protein-magnetic metal, and reacting to prepare the magnetic nanoparticles coated with water-soluble protein.

[0027] Since the outer surface of the magnetic metal nanoparticles prepared by the present invention is coated with protein, no additional surface amination or carboxylation is required, and targeting molecules can be directly modified, which is suitable for in vivo imaging, cancer-specific targeting, and In vivo signal real-time tracking, etc.

[0028] In the present invention, the magnetic metal can be iron, cobalt, nickel, chromium, manganese, etc.; the protein can be one of bovine serum albumin, human seru...

preparation example 1

[0042](1) A certain quality of bovine serum albumin was dissolved in water to prepare a solution of 20 mg / mL;

[0043] (2) Get the chloride salt of ferrous iron and ferric iron to prepare iron ion mixed solution, the mixing molar ratio of ferric iron and ferrous iron is 1:2, and the total concentration of iron element is 100mmol / L;

[0044] (3) Under the condition of magnetic stirring, add 0.8mL iron salt mixed solution into 10mL protein aqueous solution, fully react for 30 minutes, and obtain the intermediate dispersion product of protein-iron;

[0045] (4) Add 1 mL of aqueous sodium hydroxide solution with a concentration of 1 mol / L to the protein-iron intermediate dispersion product obtained in step (3), and stop the reaction after stirring for 8 hours in a water bath at 37°C.

[0046] (5) The product obtained in step (4) was purified by dialysis for 72 hours. The dialysis bag used had a molecular weight cut-off of 3500, and the water was changed every 12 hours.

[0047] (...

preparation example 2

[0050] (1) A certain quality of human serum albumin was dissolved in water to prepare a 0.5 mg / mL solution.

[0051] (2) get cobalt chloride and prepare cobalt ion solution, the total concentration of cobalt element is 0.5mmol / L;

[0052] (3) Under magnetic stirring conditions, add 10 mL of cobalt salt solution into 10 mL of protein aqueous solution, and fully react for 1 hour to obtain an intermediate dispersion product of protein-cobalt;

[0053] (4) Add 0.2 mL of 1 mol / L sodium hydroxide aqueous solution to the protein-cobalt intermediate dispersion product obtained in step (3), and stop the reaction after stirring for 5 hours in a water bath at 37°C.

[0054] (5) The product obtained in step (4) was purified by dialysis for 72 hours. The dialysis bag used had a molecular weight cut-off of 12,000, and the water was changed every 12 hours.

[0055] (6) The dialyzed product is centrifuged and concentrated to obtain the final protein-coated cobalt oxide nanoparticle powder sa...

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Abstract

The invention provides magnetic nano-particles coated with water-soluble protein and a preparation method of the magnetic nano-particles. The preparation method comprises the following steps: mixing protein with a magnetic metal salt to prepare an intermediate dispersion product of a protein-magnetic metal, wherein metal ions of the magnetic metal salt are compound with the protein; and adding alkali into the intermediate dispersion product of the protein-magnetic metal to prepare the magnetic nano-particles coated with the water-soluble protein after reaction. The raw materials used in the preparation method of the magnetic nano-particles coated with the water-soluble protein are low in price, easily-available, natural, nontoxic and environment-friendly, and have biological applicability; and the obtained product is externally coated with the protein, is good in water solubility and high in biological activity, can be further used for modifying targeting molecules easily, is extra small in size, can enter histiocytes easily, and is suitable for biological medical applications of living body imaging, cancer specific targeting, in vivo signal real-time tracing and the like.

Description

technical field [0001] The invention relates to a nanometer particle which can be used in the field of biomedical imaging and a preparation method thereof, in particular to a water-soluble protein-coated magnetic nanometer particle and a preparation method thereof. Background technique [0002] Magnetic nanoparticles are a research hotspot in recent years, and are widely used in various industries due to their uniform and excellent surface physical and chemical properties. Nanoparticles with superparamagnetic iron oxide (SPIO) are the best choice for future cancer diagnosis and treatment, and can be used in diagnostic experiments, MRI contrast agents, cell differentiation, hyperthermia, and drug loading. [0003] Bottom-up synthesis methods have been widely used in the preparation of magnetic iron oxide particles in the past decades. Such as co-precipitation, thermal decomposition, sol-gel method, hydrothermal reaction, etc. At present, the preparation methods based on fer...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/18A61K49/14A61K49/04A61K9/14A61K47/42
Inventor 高学云王亚玲
Owner INST OF HIGH ENERGY PHYSICS CHINESE ACAD OF SCI
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