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Preparation and Application of a Ratio-type Fluorescent Oxygen Sensing Membrane

An oxygen sensing film, ratio-type technology, applied in the field of sensing, can solve the problems of reducing stability, sensitivity and accuracy, and achieve the effects of high sensitivity and accuracy, large detection range and long excitation life

Active Publication Date: 2018-06-05
XIAMEN MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The luminous intensity of the luminescent sensing substance is not only affected by the concentration change of the analyte, but also easily affected by other factors such as its own distribution uniformity and attenuation degree, light source stability, and test environment, which reduces the stability of its detection. performance, sensitivity and accuracy, need to be corrected from time to time, which limits its application

Method used

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  • Preparation and Application of a Ratio-type Fluorescent Oxygen Sensing Membrane
  • Preparation and Application of a Ratio-type Fluorescent Oxygen Sensing Membrane
  • Preparation and Application of a Ratio-type Fluorescent Oxygen Sensing Membrane

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Prepare 4 mg / mL meso-(pentafluorophenyl)porphyrin platinum complex (PtFTPP) in tetrahydrofuran (THF) and 4,4'-bis(2-benzoxazolyl)stilbene (BBS) Tetrahydrofuran (THF) saturated solution, the two are mixed in a volume ratio of 30:1 to prepare a fluorescent indicator solution;

[0033] Under ultrasonic conditions, mix tetramethoxysilane, dimethyldimethoxysilane and 0.01mol / L HCl at a volume ratio of 1:1.5:1.5, place the mixture in a water bath at 60°C and stir for 2 hours at constant temperature, and remove the lower layer Mix the oily solution and the fluorescent indicator solution at a ratio of 4:1, stir for 5 minutes to obtain a fluorescent gel solution, take the fluorescent gel solution to form a film on the treated glass slide by spin coating or drop coating, and dry it in an oven at 80°C for 20 hours Oxygen sensing membrane was prepared.

Embodiment 2

[0035] Prepare 2 mg / mL meso-(pentafluorophenyl)porphyrin platinum complex (PtFTPP) in tetrahydrofuran (THF) and 4,4'-bis(2-benzoxazolyl)stilbene (BBS) Tetrahydrofuran (THF) saturated solution, the two are mixed in a volume ratio of 10:1 to prepare a fluorescent indicator solution;

[0036] Under ultrasonic conditions, mix tetramethoxysilane, dimethyldimethoxysilane and 0.01mol / L HCl in a volume ratio of 1:1.8:1.5, place the mixed solution in a water bath at 50°C and stir for 3 hours, and take the lower layer of oil Fluorescent solution and fluorescent indicator solution were mixed at a ratio of 2:1, stirred for 10 minutes to obtain a fluorescent gel solution, and the fluorescent gel solution was taken to form a film on the treated glass slide by spin coating, dip coating, drop coating or pull-up method, and then dried in an oven. The oxygen sensing membrane was prepared by drying at 60°C for 30 h.

Embodiment 3

[0038] Prepare 8 mg / mL meso-(pentafluorophenyl)porphyrin platinum complex (PtFTPP) in tetrahydrofuran (THF) and 4,4'-bis(2-benzoxazolyl)stilbene (BBS) Tetrahydrofuran (THF) saturated solution, the two are mixed in a volume ratio of 60:1 to prepare a fluorescent indicator solution;

[0039] Under ultrasonic conditions, mix tetramethoxysilane, dimethyldimethoxysilane and 0.01mol / L HCl in a volume ratio of 1:2:1.5, place the mixture in a water bath at 80°C and stir for 1 hour, and take the lower layer of oil Fluorescent solution and fluorescent indicator solution were mixed at a ratio of 6:1, stirred for 15 minutes to obtain a fluorescent gel solution, and the fluorescent gel solution was taken to form a film on the treated glass slide by spin coating or drop coating, and dried in an oven at 90°C for 15 hours. Oxygen sensing membrane.

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Abstract

The invention discloses a sol-gel method to immobilize two luminescent substances that can be excited by the same wavelength in a sensing film at the same time, wherein PtFTPP has a sensitive response to changes in oxygen concentration and can be used as an indicating probe, while the fluorescent The whitening agent BBS has no obvious response to oxygen and has good photostability. It can be used as a reference substance to construct a ratio-type oxygen sensing film, and detect the concentration of oxygen and dissolved oxygen through the fluorescence ratio detection method. High sensitivity and accuracy, quick response and good stability.

Description

technical field [0001] The invention relates to the field of sensing technology, in particular to the preparation and application of a ratio-type fluorescent oxygen sensing film. Background technique [0002] The determination of oxygen in biomass is of great significance in the fields of industrial production, disease diagnosis, and environmental detection. Therefore, oxygen sensing and detection technology has also been developed accordingly. The reported oxygen detection methods include titration, amperometry, chemiluminescence and optical oxygen sensing. Among them, optical oxygen sensing has the advantages of no oxygen consumption and no electronic interference during the measurement process, and has been successfully applied in the fields of chemistry, clinical and environment. [0003] The basic principle of optical oxygen sensing is to indirectly measure the concentration of oxygen by measuring the change of the luminescence intensity or luminescence decay time of t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
Inventor 叶廷秀林启凰吴兰兰罗红元
Owner XIAMEN MEDICAL COLLEGE
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