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Preparation and application of ratio--type fluorescent oxygen sensing film

An oxygen sensing film, ratio-type technology, applied in the field of sensing, can solve the problems of reducing stability, sensitivity and accuracy, and achieve the effects of high sensitivity and accuracy, good stability and good embedding performance

Active Publication Date: 2016-01-27
XIAMEN MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The luminous intensity of the luminescent sensing substance is not only affected by the concentration change of the analyte, but also easily affected by other factors such as its own distribution uniformity and attenuation degree, light source stability, and test environment, which reduces the stability of its detection. performance, sensitivity and accuracy, need to be corrected from time to time, which limits its application

Method used

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  • Preparation and application of ratio--type fluorescent oxygen sensing film
  • Preparation and application of ratio--type fluorescent oxygen sensing film
  • Preparation and application of ratio--type fluorescent oxygen sensing film

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Prepare 4 mg / mL meso-(pentafluorophenyl)porphyrin platinum complex (PtFTPP) in tetrahydrofuran (THF) and 4,4'-bis(2-benzoxazolyl)stilbene (BBS) Tetrahydrofuran (THF) saturated solution, the two are mixed in a volume ratio of 30:1 to prepare a fluorescent indicator solution;

[0033] Under ultrasonic conditions, mix tetramethoxysilane, dimethyldimethoxysilane and 0.01mol / L HCl at a volume ratio of 1:1.5:1.5, place the mixed solution in a water bath at 60°C for 2 hours, and take the oily lower layer Fluorescent solution and fluorescent indicator solution were mixed at a ratio of 4:1, stirred for 5 minutes to obtain a fluorescent gel solution, and the fluorescent gel solution was spin-coated or drop-coated to form a film on the treated glass slide, and dried in an oven at 80°C for 20 hours. Oxygen sensing membrane.

Embodiment 2

[0035] Prepare 2 mg / mL meso-(pentafluorophenyl)porphyrin platinum complex (PtFTPP) in tetrahydrofuran (THF) and 4,4'-bis(2-benzoxazolyl)stilbene (BBS) Tetrahydrofuran (THF) saturated solution, the two are mixed in a volume ratio of 10:1 to prepare a fluorescent indicator solution;

[0036] Under ultrasonic conditions, mix tetramethoxysilane, dimethyldimethoxysilane and 0.01mol / L HCl at a volume ratio of 1:1.8:1.5, place the mixture in a water bath at 50°C and stir for 3 hours, and take the oily liquid in the lower layer Mix with the fluorescent indicator solution at a ratio of 2:1, stir for 10 minutes to obtain a fluorescent gel solution, and take the fluorescent gel solution to form a film on the treated glass slide by spin coating, dip coating, drop coating or pulling method, and place in an oven Dry at 60°C for 30 h to prepare the oxygen sensing membrane.

Embodiment 3

[0038] Prepare 8 mg / mL meso-(pentafluorophenyl)porphyrin platinum complex (PtFTPP) in tetrahydrofuran (THF) and 4,4'-bis(2-benzoxazolyl)stilbene (BBS) Tetrahydrofuran (THF) saturated solution, the two are mixed in a volume ratio of 60:1 to prepare a fluorescent indicator solution;

[0039] Under ultrasonic conditions, mix tetramethoxysilane, dimethyldimethoxysilane and 0.01mol / L HCl at a volume ratio of 1:2:1.5, place the mixture in a water bath at 80°C and stir for 1 hour, and remove the oily liquid in the lower layer Mix with the fluorescent indicator solution at a ratio of 6:1, stir for 15 minutes to obtain a fluorescent gel solution, take the fluorescent gel solution to form a film on the treated glass slide by spin coating or drop coating, and dry it in an oven at 90°C for 15 hours. Oxygen sensing membrane.

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Abstract

The invention discloses a sol-gel method for fixing two light-emitting substances in a sensing film at the same time, wherein the two light-emitting substances are capable of being excited by fluorescence having save wavelength; PtFTPP is sensitive to oxygen concentration change and can serve as a probe, while a fluorescent brightening agent BBS can serve as a reference substance since the fluorescent brightening agent BBS is not obviously responsive to the oxygen and is light-stable; so that a the ratio type fluorescent oxygen sensing film is constructed. By means of the oxygen sensing film, a fluorescence ratio detection method is employed for detecting oxygen and dissolved oxygen concentration; the sensing film has good sensitivity, accuracy and stability and a quick responsiveness.

Description

technical field [0001] The invention relates to the field of sensing technology, in particular to the preparation and application of a ratio-type fluorescent oxygen sensing film. Background technique [0002] The determination of oxygen in biomass is of great significance in the fields of industrial production, disease diagnosis, and environmental detection. Therefore, oxygen sensing and detection technology has also been developed accordingly. The reported oxygen detection methods include titration, amperometry, chemiluminescence and optical oxygen sensing. Among them, optical oxygen sensing has the advantages of no oxygen consumption and no electronic interference during the measurement process, and has been successfully applied in the fields of chemistry, clinical and environment. [0003] The basic principle of optical oxygen sensing is to indirectly measure the concentration of oxygen by measuring the change of the luminescence intensity or luminescence decay time of t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 叶廷秀林启凰吴兰兰罗红元
Owner XIAMEN MEDICAL COLLEGE
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