Method for preparing alanyl-glutamine dipeptide through biological enzyme conversion

Abstract

The invention relates to the technical field of the preparation for alanyl-glutamine dipeptide and particularly discloses a method for preparing alanyl-glutamine dipeptide through biological enzyme conversion. The method comprises the following steps: 1) preparing a biological enzyme liquid: dissolving the biological enzyme in a buffered liquid, controlling pH value within the scope of 7.0+ / -2.0, controlling the temperature at 5-10 DEG C, thereby acquiring the biological enzyme liquid, wherein the mass fraction of the ester acyl-containing transferring biological enzyme is 5%-20%; 2) dissolving the ester derivates of substrate L-alanine and glutamine in pure water, and then uniformly dropping the biological enzyme liquid into a reaction liquor, and meanwhile, adding acid liquor or alkali liquor, controlling the pH of the reaction liquor at 5-10, reacting under the temperature controlled at 15-25 DEG C, sampling and detecting the production of the alanyl-glutamine dipeptide during the reaction process, stopping reaction when the content of the alanyl-glutamine dipeptide is highest, and separating the acquired converted liquid, thereby acquiring the alanyl-glutamine dipeptide. According to the method provided by the invention, the enzyme activity is high, the reaction rate of the materials is higher, the method has the advantages of simple technique control, easy control on quality index and low cost, and the method is more fit for industrial production.

Description

technical field [0001] The invention relates to the technical field of preparation of glucodipeptide. Background technique [0002] N(2)-L-alanyl-L-glutamine (also known as alanyl glutamine) is a derivative of amino acid, referred to as proglycine dipeptide, which is mainly used as amino acid parenteral nutrition and has important medical value and economic value. [0003] The preparation technology of CG dipeptide mainly includes chemical synthesis method and microbial enzyme conversion method. The chemical synthesis method mainly has the disadvantages of long synthesis route, long production cycle, large environmental pollution, strict process conditions and high cost. [0004] The patent with the publication number CN104480075A discloses a method for preparing glucodipeptide by catalytic conversion of biological enzymes. The preparation and use of the biological enzymes adopt the form of biological enzyme freeze-dried powder, which is inconvenient to use, and has low enz...

AI Technical Summary

Problems solved by technology

[0003] The preparation technology of CG dipeptide mainly includes chemical synthesis method and microbial enzyme conversion method. The chemical synthesis method mainly has the disadvantages of long synthesis route, long production cycle, large environmental pollution, strict process conditions and high cost.

[0004] The patent with the publication number CN104480075A discloses a method for preparing glutamic acid dipeptide by catalytic conversion of biological enzymes. The preparation and use of biological enzymes adopt the form of biological enzyme freeze-dried powder, which is inconvenient to use, and has low enzyme activity and low material reaction rate.

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