DNA sequencer

Abstract

The invention relates to a DNA sequencer, comprising a reaction recording subsystem, a reagent supply system and a control system, and a sequencer box which bears the above systems, wherein the control system is disposed at the rear of the reaction recording subsystem disposed a left area of the box and controls the reaction subsystem and the reagent supply system to act according to a preset time sequence; the control system controls the reagent supply system to transfer a reagent to the reaction recording subsystem, the reagent flows through a reaction chip disposed in a reaction chamber module to react, a CCD (charge coupled device) camera of the reaction recording subsystem collects photos in a reaction process and acquires a measured DNA sequence by analyzing. During a DNA sequencing process, the sequencer necessarily features continuation and controllability during reagent supply and sequencing reaction processes, sequencing reaction and reagent supply equipment are provided with a stable, closed operating environment; the whole structure is stable, and sequencing efficiency is high.

Description

technical field [0001] The invention relates to the field of DNA sequencing, in particular to a DNA sequencer with a box structure, easy to carry, and capable of autonomously supplying liquid. Background technique [0002] In the field of DNA sequencing technology, pyrosequencing technology (pyrosequencing) is a new enzyme cascade sequencing technology developed by Nyren et al. in 1987. Its repeatability and accuracy are comparable to Sanger method DNA sequencing technology. , while the speed is greatly improved. [0003] The overall operation process is described as follows: After the DNA sample is crushed, the library construction reagent is used to add adapters, single-strand capture, bind to microspheres, microemulsion PCR amplification, and emulsion breaking to obtain a DNA library built on microspheres. The template lays the library and the enzymes required for the sequencing reaction on the sequencing chip with a micro-reaction pool, installs the sequencing chip and ...

AI Technical Summary

Problems solved by technology

[0004] In the actual DNA sequencing process, the sequencer is required to have continuous and controllable characteristics in the reagent supply and sequencing reaction process, and the sequencing reaction and reagent supply equipment should have a stable and airtight operating environment; the existing DNA Sequencing equipment is difficult to meet the above needs

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