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Method for extracting boletus polysaccharide from boletus

A boletus and polysaccharide technology, applied in the field of biomedicine, can solve the problems of less extraction research, achieve high extraction efficiency, high content of active ingredients, and shorten the production cycle

Inactive Publication Date: 2016-01-27
GUANGXI NANNING SHENGQIAN TECH DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the reports on boletus at home and abroad mainly focus on proteins, and there are few studies on the extraction of boletus polysaccharides in boletus.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Weigh 100kg of dried boletus and crush it to less than 0.5cm, put it into the extraction tank, add 500L of 0.3% hydrochloric acid solution, and start stirring; open the jacket and steam to heat to 40°C, start timing, extract for 3 hours, and feed the liquid through 400 mesh filter bag; after the reflux extraction, the feed liquid passes through a 400-mesh filter bag, the filtrate is added with calcium hydroxide to adjust the pH value to 7.5, and centrifuged; the centrifuged liquid is added with acetic acid to adjust the pH value to 5.0, and centrifuged; The flow rate was added to the 100L macroporous adsorption resin column, and the macroporous adsorption resin was washed with water at a flow rate of 120L / h to remove unadsorbed impurities; Porous adsorption resin to remove impurities with higher polarity; then use 65% ethanol solution as the desorption liquid to wash the macroporous adsorption resin at a flow rate of 50L / h, collect the desorption liquid after washing, co...

Embodiment 2

[0032] Weigh 100kg of dried boletus and crush it below 0.5cm, put it into the extraction tank, add 300L of 0.25% hydrochloric acid solution, and start stirring; open the jacket and steam to heat to 30°C, start timing, extract for 2 hours, and feed the liquid through 400 mesh filter bag; after the reflux extraction is completed, the feed liquid passes through a 400-mesh filter bag, the filtrate is added with calcium hydroxide to adjust the pH value to 8.0, and centrifuged; the centrifuged liquid is added with acetic acid to adjust the pH value to 4.5, and centrifuged; The flow rate was added to the 100L macroporous adsorption resin column, and the macroporous adsorption resin was washed with water at a flow rate of 100L / h to remove unadsorbed impurities; Porous adsorption resin to remove impurities with higher polarity; then use ethanol solution with a volume fraction of 70% as the desorption liquid to wash the macroporous adsorption resin at a flow rate of 40L / h, collect the de...

Embodiment 3

[0034] Weigh 100kg of dried boletus and crush it to less than 0.5cm, put it into the extraction tank, add 400L of 0.35% hydrochloric acid solution, and start stirring; open the jacket and steam to heat to 35°C, start timing, extract for 1 hour, and feed the liquid through 400 mesh filter bag; after the reflux extraction is completed, the feed liquid passes through a 400-mesh filter bag, the filtrate is added with calcium hydroxide to adjust the pH value to 7.8, and centrifuged; the centrifuged liquid is added with acetic acid to adjust the pH value to 4.8, and centrifuged; The flow rate was added to the 100L macroporous adsorption resin column, and the macroporous adsorption resin was washed with water at a flow rate of 200L / h to remove unadsorbed impurities; Porous adsorption resin to remove impurities with higher polarity; then use 65% ethanol solution as the desorption solution to wash the macroporous adsorption resin at a flow rate of 60L / h, collect the desorption solution ...

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PUM

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Abstract

The invention discloses a method for extracting a boletus polysaccharide from boletus. The method comprises the following steps: (1) extracting the boletus with a 0.25-0.35 percent by mass hydrochloric acid solution at 30-40 DEG C for 1-3 hours to obtain an extracting solution; (2) adding an alkali into the extracting solution to adjust a pH value to 7.5-8.0, and performing solid-liquid separation to remove insoluble substances so as to obtain a clear aqueous solution A; (3) adding an acid into the aqueous solution A to adjust the pH value to 4.0-4.5, and performing solid-liquid separation to remove insoluble substances in order to obtain a clear aqueous solution B; (4) adding the aqueous solution B into a macroporous adsorption resin column, and washing the macroporous adsorption resin column with water to remove non-adsorbed impurities; washing the macroporous adsorption resin column with a 15-20 percent by volume ethanol solution to remove high-polarity impurities; washing the macroporous adsorption resin column with a 65-70 percent by volume ethanol solution serving as a desorption solution, collecting the washed desorption solution, and performing concentration under a reduced pressure and drying to obtain a boletus extract. The method is reasonable in process, high in extraction efficiency, easy to operate, and suitable for industrial production.

Description

technical field [0001] The invention relates to a method for extracting boletus polysaccharide from boletus, and the invention relates to the field of biomedicine. Background technique [0002] Boletus (scientific name: Boletus) is a collective name for fungi such as Boletaceae and Pine Cone Boletus. It is a wild and edible mushroom. Except for a few species that are poisonous or bitter and cannot be eaten, most of them are All varieties are edible. There are mainly white, yellow and black boletus. [0003] Boletus contains 8 kinds of amino acids necessary for the human body, and also contains alkaloids such as adenine, choline and putrescine. It can be used medicinally to treat pain in the waist and legs, numbness in the hands and feet, twitching of the limbs, and it can also be used to treat abnormal leucorrhea in women. It has the functions of clearing heat and relieving troubles, nourishing blood and harmonizing neutralization, chasing wind and dispersing cold, relaxi...

Claims

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Application Information

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IPC IPC(8): C08B37/00A23L33/125
Inventor 陈先锐
Owner GUANGXI NANNING SHENGQIAN TECH DEV CO LTD
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