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Improved cabbage type oilseed rape radish cytoplasmic male sterility restoring line and improvement method thereof

A cabbage rape, male sterile line technology, applied in the directions of plant genetic improvement, botanical equipment and methods, applications, etc., can solve problems such as long chromosome fragments of exogenous radish, and achieve the effect of restoring fertility

Inactive Publication Date: 2016-01-20
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the exogenous radish chromosome segment of the restorer line R2000 is still too long, which has a great impact on its agronomic traits and yield performance

Method used

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  • Improved cabbage type oilseed rape radish cytoplasmic male sterility restoring line and improvement method thereof
  • Improved cabbage type oilseed rape radish cytoplasmic male sterility restoring line and improvement method thereof
  • Improved cabbage type oilseed rape radish cytoplasmic male sterility restoring line and improvement method thereof

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Embodiment 1

[0044] An improved method for an improved cytoplasmic male sterile restorer line of Brassica napus radish, comprising the following steps (the flow chart is shown in figure 1 ):

[0045] (1) Using the rapeseed radish cytoplasmic male sterile line Fu58Drakkar (hereinafter referred to as the sterile line Fu58Drakkar) as the female parent and the Brassica radish cytoplasmic male sterile restorer line R2000 (hereinafter referred to as the restorer line R2000) as the male parent, cross get F 1 substitute seeds;

[0046] In this example, in order to expand the provenance of the female parent and the male parent, the maintainer line G142 (the self-fertilizing material of the Rapeseed Breeding Group of the Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, and later renamed as Zheyou 50) was used to remove the The breeding line Fu58Drakkar was maintained for one generation, and the restorer line R2000 was selfed for one generation; the s...

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Abstract

The invention discloses an improved cabbage type oilseed rape radish cytoplasmic male sterility restoring line and an improvement method thereof. The improvement method comprises the following steps: by taking a cabbage type oilseed rape Ogura CMS sterile line as a female parent and taking a cabbage type oilseed rape Ogura CMS restoring line as a male parent, hybridizing to obtain generation F1 seeds; carrying out radiation treatment on the generation F1 seeds and sowing the seeds to obtain generation F1 plants after the radiation treatment; by taking the cabbage type oilseed rape Ogura CMS sterile line as a female parent and taking the generation F1 plants as a male parent, hybridizing to obtain seeds; sowing the seeds to obtain later-generation plants; and carrying out single-plant molecular marker screening on fertile plants in the later-generation plants to obtain an improved cabbage type oilseed rape Ogura CMS restoring line. According to the improved cabbage type oilseed rape radish cytoplasmic male sterility restoring line and the improvement method thereof, molecular markers, which are tightly interlocked with restoring genes, in exogenous radish chromosome segments are eliminated, and are screened to obtain the improved restoring line with relatively short exogenous radish chromosome segments; and the improved restoring line has a good agricultural shape and a relatively high grain yield.

Description

technical field [0001] The invention belongs to the technical field of crop breeding, and in particular relates to an improved cytoplasmic male sterile restorer line of cabbage-type rape radish and an improvement method thereof. Background technique [0002] The restorer line of radish cytoplasmic male sterility (oguracytoplasmic male sterility, oguraCMS) was first discovered in radish (Raphanus sativus) resources in 1968 (OguraH. Studies on male sterility in Japanese radish with special reference to the utilization of this sterility toward the practical raising of hybridseed [J]. MemFacAgricKagoshima, 7, 7 彻底,是由细胞质中线粒体嵌合基因Orf138(BrownGG,FormanovaN,JinH,etalTheradishRforestorergeneofOguracytoplasmicmalesterilityencodesaproteinwithultiplepentatricopeptiderepeats[J].PlantJ,2003,35(2):262-272.;DesloireS,GherbiH,LalouiW,etal.Identificationofthefertilityrestorationlocus,Rfo,inradish , as a member of the pentatricopeptide-repeat protein family [J]. EMBORep, 2003, 4(6):588-594.) and...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04A01H1/06
Inventor 华水金张冬青张永平
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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