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Gossypium barbadense GbHyPRP1 gene promoter and application thereof

A promoter, island technology, applied in applications, angiosperms/flowering plants, DNA/RNA fragments, etc.

Active Publication Date: 2016-01-13
HEBEI AGRICULTURAL UNIV.
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Yet, before the research of the present invention, there is no report of cotton HyPRP protein gene promoter and its regulatory elements

Method used

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  • Gossypium barbadense GbHyPRP1 gene promoter and application thereof
  • Gossypium barbadense GbHyPRP1 gene promoter and application thereof
  • Gossypium barbadense GbHyPRP1 gene promoter and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. Test materials

[0030] 1.1 Plant material

[0031] The cotton species tested was Pima90-53, a variety resistant to Verticillium wilt. The Columbia ecotype used for transgenic Arabidopsis.

[0032] 1.2 Strains and vectors

[0033] Escherichia coli TOP10 competent cells (CB104) were purchased from Tiangen Biochemical Technology (Beijing) Co., Ltd. The T cloning vector pEASY-T1Simple was purchased from Beijing Quanshijin Biotechnology Co., Ltd. Agrobacterium GV3101 and plant overexpression vector pBI121 are preserved by our laboratory.

[0034] 1.3 Main reagents

[0035] GenomeWalkingKit was purchased from Treasure Bioengineering (Dalian) Co., Ltd.; 5-bromo-4-chloro-3-indole-β-D-glucuronide (X-Gluc) was purchased from Sigma-Aldrich China; PlantGenomicDNAKit was purchased from Beijing Quanshijin Biotechnology Co., Ltd. E.Z.N.A. GelExtraction Kit was purchased from OmegaBio-tek, USA. 2×EsTaqMasterMix (containing dye) was purchased from Kangwei Century Biotechn...

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PUM

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Abstract

The invention relates to a gene promoter, and particularly discloses a gossypium barbadense GbHyPRP1 gene promoter. A nucleotide sequence of the gene promoter is as shown in SEQ ID No.1 or is a nucleotide sequence formed by deriving from the sequence as shown in SEQ ID No.1 encoding the same functional amino acid sequence and substituting, deleting and / or adding one or more nucleotide sequences as shown in SEQ ID No.1. Elements associated to the control of hormones and effectors in the promoter are verified by virtue of experiments; and the gene promoter has capacity for promoting the expression of GUS genes and promotes the genes on roots and tips.

Description

technical field [0001] The present invention relates to a gene promoter, in particular to the GbHyPRP1 gene promoter of Sea Island cotton. Background technique [0002] Cotton is not only the most important fiber crop, but also an important economic crop, and its production has a huge impact on the global economy. The history of human cotton planting can be traced back to 5,000-10,000 years ago. At present, more than 80 countries around the world are commercially planting cotton, such as China, the United States, India, Australia and so on. China is the world's largest producer and consumer of lint. About 100 million households around the world are engaged in cotton-related industries, with an annual output value of about US$100 billion. [0003] As the main cultivated species, upland cotton is one of the important hosts of Verticillium wilt fungus. Typically, Verticillium wilt can cause 10% to 20% loss of cotton yield, and more than 60% in severe cases. Currently, ther...

Claims

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Application Information

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IPC IPC(8): C12N15/113C12N15/10C12N15/82A01H5/00
Inventor 马峙英杨君王省芬张艳吴金华李志坤吴立强张贵寅
Owner HEBEI AGRICULTURAL UNIV.
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