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A kind of method that utilizes microorganism to ferment lignocellulosic raw material to produce ethanol

A technology of lignocellulose and microbial fermentation, applied in the field of microbial fermentation, can solve the problem of low conversion rate

Active Publication Date: 2019-12-03
COFCO NUTRITION & HEALTH RES INST +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Therefore, the object of the present invention is to overcome the defect of low conversion rate in actual production caused by the different consumption requirements of fermentation supply in the process of microbial metabolism of lignocellulosic raw material enzymolyzate containing C5 and C6 sugars in the prior art, Thereby providing a method for efficiently utilizing microorganisms to ferment lignocellulosic raw materials to produce ethanol, thereby increasing the conversion rate of ethanol

Method used

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  • A kind of method that utilizes microorganism to ferment lignocellulosic raw material to produce ethanol

Examples

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Embodiment 1

[0071] This embodiment provides an expanded culture method for co-fermenting C5 and C6 microorganisms to produce ethanol. Taking recombinant S. ) (such as Cellulosic ethanol production from AFEX-treated corn stover using Saccharomyces cerevisiae424A(LNH-ST), M.W.Lau, B.E.Dale, ProcNatl Acad Sci USA, 106(2009), pp.1368-1373; Two-step SSCF to convert AFEX- Treated switchgrass to ethanol using commercial enzymes and Saccharomycescerevisiae424A (LNH-ST), M.Jin, M.W.Lau, V.Balan, B.E.Dale, Bioresour Technol, 101(2010), pp.8171–8178, etc. reported), for commercially available , the expanded cultivation method adopts a first-level expanded cultivation step, specifically comprising:

[0072] A. Seed liquid culture steps

[0073] Prepare YEPD medium, inoculate the preserved above bacteria into YEPD medium, control the temperature at 25°C, pH 6.0, rotating speed at 200rpm, ventilation rate at 0.2L / L.min, and cultivate for 12h until the concentration of the above bacteria is (0.1 -0.5)...

Embodiment 2

[0079] This example provides an expanded culture method for microorganisms that co-ferment C5 and C6 to produce ethanol. Taking Candida tropicalis as an example to carry out the expanded culture experiment, the Candida tropicalis is commercially available, and the expanded culture The method adopts two-stage expanded culture steps, which specifically include:

[0080] A. Seed liquid culture steps

[0081] Prepare the YEPD medium, inoculate the preserved above bacteria into the YEPD medium, control the temperature at 25°C, pH6.0, rotating speed at 200rpm, air flow at 0.2L / L.min, and cultivate until the concentration of the above bacteria is (0.1-0.5 )×10 9 / ml;

[0082] The YEPD medium specifically contains 10 g / L of yeast powder, 20 g / L of peptone, and 20 g / L of glucose, and the pH is adjusted to 6.0.

[0083] B. Expand the cultivation steps

[0084] 1) Put the cultured seed solution into a 50L primary expansion tank containing a primary expansion medium according to the i...

Embodiment 3

[0088] This embodiment provides a kind of expanded culture method of co-fermenting C5 and C6 to produce ethanol microorganisms, take recombinant S. Three-level expanded cultivation steps, specifically including:

[0089] A. Seed liquid culture steps

[0090] Prepare YEPD medium, inoculate the preserved above bacteria into YEPD medium, control the temperature at 30°C, pH 6.0, ventilation rate 0.2L / L.min, rotation speed 200rpm, and cultivate for 14h until the concentration of the above bacteria is (0.2 -0.3)×10 9 / ml;

[0091] The YEPD medium specifically contains 10 g / L of yeast powder, 20 g / L of peptone, and 20 g / L of glucose, and the pH is adjusted to 6.5.

[0092] B. Expand the cultivation steps

[0093] 1) Put the cultured seed solution into a 50L primary expansion tank containing a primary expansion medium according to the inoculum amount of 10% (v / v), control the temperature at 30°C, pH 5.0, and rotate at 200rpm Ventilate the air, the ventilation rate is 0.5L / L.min, ...

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Abstract

The invention provides a method for producing ethanol from a microbial fermentation lignocellulose raw material. The method comprises carrying out enzymolysis on a lignocellulose raw material to obtain a lignocellulose raw material enzymatic hydrolysate, carrying out microbial enlarging cultivation to obtain an enlarging cultivation solution, inoculating the lignocellulose raw material enzymatic hydrolysate with the enlarging cultivation solution, and adjusting fermentation by intermittent oxygen supply. The method greatly improves an ethanol yield.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to a method for producing ethanol by using microorganisms to ferment lignocellulosic raw materials. Background technique [0002] The depletion of many non-renewable fossil energy sources such as oil has drawn more and more attention to renewable energy, especially biofuels, and has brought huge business opportunities and social significance. [0003] According to the relevant definitions of the International Energy Agency (IEA), conventional biofuels include sugar-based and starch-based ethanol, oil crop-based biodiesel, directly usable vegetable oils, and biogas produced by anaerobic digestion; Transformation technologies in the R&D, pilot or demonstration stages are usually referred to as second-generation technologies or third-generation technologies. This category includes hydrogenated vegetable oils (HVO) produced from animal fats and vegetable oils, as well ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14C12P7/10C12R1/865C12R1/645C12R1/84C12R1/72C12R1/74C12R1/18C12R1/22C12R1/145C12R1/77C12R1/19
CPCY02E50/10
Inventor 沈乃东熊强李凡苏会波彭超武国庆林鑫林海龙袁敬伟李春玲刘文信
Owner COFCO NUTRITION & HEALTH RES INST
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