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Optimized culture method of Dunaliella salina

A technology of Dunaliella salina and its cultivation method, which is applied in the field of algae cultivation and can solve problems such as unsatisfactory cultivation of Dunaliella salina

Active Publication Date: 2015-12-30
QINGDAO KEHAI BIOLOGICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the cultivation of salina in the prior art is not ideal, especially the extraction rate of β-carotene in the cultivated salina is only about 8%-10%

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] In the present invention, the optimized cultivation method of Dunaliella salina specifically comprises the following steps:

[0020] Step 1. Weigh 0.3g of NaNO 3 , 0.025g of KH 2 PO 4 , 1mL of Fe-EDTA mother liquor (from 15g of Na 2 EDTA and 1.0 g FeCl 3 prepared), 1mL of trace element mother solution (made from 5g of H 3 BO 3 , 3g of MnCl 2 , 0.1g of ZnCl 2 , 3mg of CuCl 2 and 4mg of CoCl 2 prepared) and 1mL of vitamin stock solution (consisting of 0.5mg of B 12 prepared); the above-mentioned prepared medium is controlled to pH 7 by adding acid or alkali; the preparation process of the above-mentioned medium is completed in a closed photobioreactor, and the culture density of the closed photobioreactor is High, easy to control the culture conditions;

[0021] Step 2. Select the well-developed Dunaliella salina strain in the exponential growth phase, inoculate it into the culture medium prepared in step 1, the inoculation amount is 5%, place it in an incubato...

Embodiment 2

[0024] In the present invention, the optimized cultivation method of Dunaliella salina specifically comprises the following steps:

[0025] Step 1. Weigh 0.5g of NaNO 3 , 0.025g of KH 2 PO 4 , 1mL of Fe-EDTA mother liquor (from 30g of Na 2 EDTA and 1.0 g FeCl 3 Prepared), 1mL of trace element mother solution (from 10g of H 3 BO 3 , 1g of MnCl 2 , 0.1g of ZnCl 2 , 1mg of CuCl 2 and 2mg of CoCl 2 prepared) and 1mL of vitamin stock solution (consisting of 0.5mg of B 12 prepared); the above-mentioned prepared medium is controlled to have a pH of 8 by adding acid or alkali; the preparation process of the above-mentioned medium is all completed in a closed photobioreactor, and the closed photobioreactor culture density High, easy to control the culture conditions;

[0026] Step 2. Select the well-developed Dunaliella salina strain in the exponential growth phase, inoculate it into the medium prepared in step 1, the inoculation amount is 5%, place it in an incubator for cu...

Embodiment 3

[0029] In the present invention, the optimized cultivation method of Dunaliella salina specifically comprises the following steps:

[0030] Step 1. Weigh 0.3g of NaNO 3 , 0.01g of KH 2 PO 4 , 1mL of Fe-EDTA mother liquor (from 15g of Na 2 EDTA and 0.5 g FeCl 3 prepared), 1mL of trace element mother solution (made from 5g of H 3 BO 3 , 3g of MnCl 2 , 0.2g of ZnCl 2 , 3mg of CuCl 2 and 3mg of CoCl 2 prepared) and 1mL of vitamin stock solution (consisting of 0.5mg of B 12 prepared); the above-mentioned prepared medium is controlled to have a pH of 8 by adding acid or alkali; the preparation process of the above-mentioned medium is all completed in a closed photobioreactor, and the closed photobioreactor culture density High, easy to control the culture conditions;

[0031] Step 2. Screen out the well-developed Dunaliella salina strain in the exponential growth phase, inoculate it into the culture medium prepared in step 1, the inoculation amount is 5%, place it in an i...

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Abstract

The invention discloses an optimized culture method of Dunaliella salina. According to the method, firstly, a culture medium is prepared in a closed photobioreactor, and the prepared culture medium contains 0.3-0.5 g of NaNO3, 0.01-0.025 g of KH2PO4, 1 mL of Fe-EDTA mother liquor, 1 mL of microelement mother liquor and 1 mL of vitamin mother liquor; then a batch of Dunaliella salina strains cultured to the exponential growth phase are screened, and the culture medium is inoculated with the strains with the inoculation amount ranging from 5% to 15%; finally, culture is performed at the temperature controlled to range from 23 DEG C to 25 DEG C with the illumination intensity of 2000-3000 lx and the illumination time of 12h:12h alternately for 15-20 days, and a culture is obtained. Beta-carotene in the Dunaliella salina cultured with the method is extracted with an ultrasonic method, and the content of the beta-carotene is detected to be increased to be higher than 14.45%.

Description

technical field [0001] The invention relates to the technical field of algae cultivation, in particular to an optimized cultivation method for Dunaliella salina. Background technique [0002] Dunaliella salina is a kind of halophilic green microalgae belonging to the order Volvox Chlorophyceae, which is commonly found in sea salt fields. Dunaliella salina is rich in oil, β-carotene, protein, polysaccharide, etc. It also contains high Ca, P, Zn and other minerals, and also contains 18 kinds of amino acids including human essential amino acids. The accumulated glycerol is dry 40%-50% of the weight. Under appropriate conditions, β-carotene synthesized in the body can reach 10% of the dry weight of cells. [0003] Salina has the following common functions: (1) scavenging free radicals, β-carotene, selenium, vitamin E and other antioxidant effects in salinadin can scavenge free radicals, protect cells from free radicals, protect cell membranes, and enhance (2) Regulating acid-...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
Inventor 李悦明邹宁徐建春夏修峦李霞宋宛霖
Owner QINGDAO KEHAI BIOLOGICAL
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