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A kind of purification method of blackberry anthocyanin

A technology of blackberry anthocyanin and purification method, applied in the field of purification of blackberry anthocyanin

Inactive Publication Date: 2017-09-22
GUIZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the separation and purification of blackberry anthocyanins using polyamide resins

Method used

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  • A kind of purification method of blackberry anthocyanin
  • A kind of purification method of blackberry anthocyanin
  • A kind of purification method of blackberry anthocyanin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0126] The purification method of embodiment 1 blackberry anthocyanin, comprises the following steps:

[0127] (1) Resin pretreatment: boil the polyamide resin with 60% ethanol for 5 hours, then wash it with distilled water until it has no alcohol smell, and set it aside;

[0128] (2) Purification of blackberry anthocyanin crude extract (prepared according to the prior art method, the content of blackberry anthocyanin in the crude extract extract is about 2.8%): the blackberry anthocyanin crude extract is diluted with water to 0.6mg·mL -1 , after adjusting pH=5 with hydrochloric acid, go up polyamide resin column 7BV with the speed of 2BV / h, wash 3BV with water subsequently (wash to almost no sugar), then use the 40% ethanol that pH value is 1 with the speed of 2BV / h The 5BV was eluted and concentrated to obtain the extract.

[0129] With this purification process, the adsorption rate of blackberry anthocyanins is 81%, the elution rate is 70%, and the content of blackberry a...

Embodiment 2

[0130] The purification method of embodiment 2 blackberry anthocyanins comprises the following steps:

[0131] (1) Resin pretreatment: first boil 60-100 mesh polyamide resin with 80% ethanol for 3 hours, then wash with distilled water until there is no alcohol smell, and set aside;

[0132] (2) Purification of crude blackberry anthocyanin extract: the crude blackberry anthocyanin extract was concentrated to evaporate ethanol and then diluted with water to 2.99 mg·mL -1 , after adjusting the pH=3 with hydrochloric acid, put 1BV of the polyamide resin column at a speed of 10BV / h, then wash 3BV with water (washing to almost no sugar), and then use 100% ethanol with a pH value of 5 at a speed of 10BV / h Elute 0.5BV and concentrate to obtain extract. With this purification process, the adsorption rate of blackberry anthocyanins is 78%, the elution rate is 65%, and the content of blackberry anthocyanins in the purified extract is 60%.

[0133] The blackberry anthocyanin crude extra...

Embodiment 3

[0134] The purification method of embodiment 3 blackberry anthocyanins comprises the following steps:

[0135] (1) Resin pretreatment: 80-mesh polyamide resin was first boiled with 95% ethanol for 4 hours, then washed with distilled water until there was no alcohol smell, and set aside;

[0136] (2) Purification of blackberry anthocyanin crude extract: the blackberry anthocyanin crude extract was concentrated to evaporate ethanol and then diluted with water to 0.75 mg·mL -1 , after adjusting pH=1 with hydrochloric acid, go up polyamide resin column 5BV with the speed of 5BV / h, wash 3BV with water subsequently (wash to almost no sugar), then use the 80% ethanol that pH value is 3 with the speed of 5BV / h The 3.5BV was eluted, concentrated by distillation under reduced pressure to obtain the extract. With this purification process, the adsorption rate of blackberry anthocyanins is 85%, the elution rate is 78%, and the content of blackberry anthocyanins in the purified extract is...

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Abstract

The invention discloses a purification method for blackberry anthocyanin. The purification method includes the steps of resin pretreatment and blackberry anthocyanin crude extract purification. Efficient purification of the blackberry anthocyanin can be achieved, and the purity of the obtained blackberry anthocyanin is high. According to the purification method, polyamide resin is selected as adsorbent and has the advantages that the large specific area and the large granularity are achieved, adsorption and desorption capacity is high, cost is low, regeneration is easy, and a preparation method is simple. Through research on the static and dynamic adsorption and desorption process of the blackberry anthocyanin, purification conditions are optimized, the initial concentration of the blackberry anthocyanin, the pH value of adsorption liquid, the pH value of eluent and the optimal value of ethanol concentration are determined, and the adsorption rate and the elution rate of the blackberry anthocyanin are both high. According to the method, time-saving and efficient purification of the blackberry anthocyanin can be achieved, purification time can be shortened, and cost can be reduced.

Description

technical field [0001] The invention relates to a method for purifying blackberry anthocyanins, belonging to the technical field of plant extraction and purification. Background technique [0002] The method of extracting anthocyanins from plants is a non-selective extraction method. In addition to pigments, a large number of other compounds will be produced, such as sugars, sugar alcohols, organic acids, amino acids, proteins, etc., which will affect the pigment. stability. Therefore, it is very necessary to purify the anthocyanins in the blackberry extract, which can effectively improve the stability of the pigment and promote its application in food processing. In the prior art, the method for purifying anthocyanins is mainly CO 2 method, column chromatography, adsorbent method and two-phase extraction technique. [0003] Adsorption is a simple and efficient method for purifying bioactive components. Adsorbents that have recently been used to purify active ingredients...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/62C09B61/00
CPCC07D311/62C09B61/00
Inventor 周欣陈华国邓青芳赵超龚小见文瑶张钰娟
Owner GUIZHOU NORMAL UNIVERSITY
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