Preparation method of organic fertilizer for bamboo forest and use method of organic fertilizer
A technology for organic fertilizers and bamboo forests, which is applied in the field of preparing organic fertilizers for bamboo forests, can solve problems such as the reduction of bamboo shoots, and achieve the effects of advancing the time of growing bamboo shoots, changing the physical structure, and killing harmful soil bacteria.
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Embodiment 1
[0031] S101: collecting bamboo forest litter with fungi in the litter layer of a healthy bamboo forest;
[0032] S102: cutting the collected litter of the bamboo forest into small pieces;
[0033] S103: Inoculate the litter and leaves of the bamboo forest cut into small pieces into the improved PDA plate medium, and cultivate them at a temperature of 22° C. until colonies grow;
[0034] S104: Select the dominant colony, transfer the hyphae at the edge of the dominant colony to a new improved PDA plate medium and continue culturing until pure mycelium grows;
[0035] S105: selecting vigorously growing pure mycelium, inoculating the pure mycelium into a modified PDA liquid medium for cultivation, and obtaining a liquid bacterial agent;
[0036] S106: inoculating the above-mentioned liquid bacterial agent into the solid compost to continue culturing to obtain a solid bacterial agent;
[0037] S107: Inoculate the above-mentioned solid bacterial agent into the expanded culture ma...
Embodiment 2
[0040] S201: collecting bamboo forest litter with fungi in the litter layer of healthy bamboo forest;
[0041] S202: cutting the collected litter of the bamboo forest into small pieces;
[0042] S203: Inoculate the litter and leaves of the bamboo forest cut into small pieces into the improved PDA plate medium, and cultivate them at a temperature of 26° C. until colonies grow;
[0043] S204: Select the dominant colony, transfer the hyphae at the edge of the dominant colony to a new improved PDA plate medium and continue culturing until pure mycelium grows;
[0044] S205: selecting vigorously growing pure mycelium, inoculating the pure mycelium into a modified PDA liquid medium for cultivation, and obtaining a liquid microbial agent;
[0045] S206: Inoculate the above-mentioned liquid bacterial agent into the solid compost to continue culturing to obtain a solid bacterial agent;
[0046]S207: Inoculate the above-mentioned solid bacterial agent into the expanded culture materia...
Embodiment 3
[0049] S301: collecting bamboo forest litter with fungi in the litter layer of a healthy bamboo forest;
[0050] S302: cutting the collected litter of the bamboo forest into small pieces;
[0051] S303: Boil bamboo litter and potatoes in boiling water for 20 minutes each, and filter to obtain bamboo litter boiling liquid with a concentration of 150 g / L and potato juice with a concentration of 150 g / L; boil the filtered bamboo litter Mix liquid, water and potato juice according to the volume ratio of 1:4:3 to obtain a mixed solution; add agar to the mixed solution, and stir while heating until the agar is completely melted, add glucose after the agar is completely melted, and stir evenly, wherein , the mass ratio of potato, glucose and agar is 15:1:1; after stirring evenly, use 0.1mol / L HCl and 0.1mol / L NaOH to adjust the pH value to 5, and carry out sterilization treatment; Cool to obtain the improved PDA plate medium. Inoculate the litter and leaves of the bamboo forest cut...
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