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Enrichment method of peppery component suitable for preparing electronic cigarette liquid

An e-cigarette liquid and spicy technology, which is applied in the fields of tobacco, application, and tobacco processing, can solve the problems of large differences in sensory evaluation and consumer dissatisfaction, and achieve the effects of wide application range, satisfying sensory requirements, and convenient operation

Inactive Publication Date: 2015-12-23
HENAN AGRICULTURAL UNIVERSITY +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] E-cigarettes use e-cigarette liquid to heat through an electronic atomizer to produce a cigarette-like mist, mainly using a mixture of tobacco-specific flavors and glycerol or propylene glycol as e-cigarette liquid, but currently e-cigarettes that use e-cigarette liquid and Consumers have always been dissatisfied with the sensory evaluation of traditional smoking cigarettes.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Enrichment method of spicy components of C3F extract from flue-cured tobacco leaves in Henan

[0022] The Henan flue-cured tobacco C3F tobacco leaves are dried in an oven, and then pulverized into tobacco powder with a pulverizer. Add 100g of tobacco powder weighed into a 1L round bottom flask, add 600mL of 75% ethanol, reflux for 4h, cool to room temperature, filter, and concentrate the filtrate on a rotary evaporator with a pressure of 50mbar and a water bath temperature of 40°C to obtain tobacco leaf extract . Weigh 15.0 g of Henan flue-cured tobacco leaf C3F extract, dissolve it in 30.0 ml of distilled water, and filter. Then, load a sample to gel chromatography, sample loading 10 milliliters, take distilled water as mobile phase, every 20 mL fractions are collected, find after tasting that N6, N7, N8, N13, N14 fractions contain spicy components. Fractions containing spicy components were combined, enriched, and freeze-dried with a lyophilizer. Dissolve the enric...

Embodiment 2

[0024] Enrichment method of spicy components in B2F extract of Henan flue-cured tobacco leaves

[0025] The B2F tobacco leaves of Henan flue-cured tobacco were dried in an oven, and then pulverized into tobacco powder with a pulverizer. Add 100g of tobacco powder weighed into a 1L round bottom flask, add 600mL of absolute ethanol, reflux for 4 hours, filter, and concentrate the filtrate on a rotary evaporator with a pressure of 50mbar and a water bath temperature of 40°C to obtain tobacco leaf extract. Weigh 3.0 g of Henan flue-cured tobacco leaf B2F extract, dissolve it in 6.0 ml of distilled water, and filter. Then, load a sample to gel chromatography, with a sample volume of 6 milliliters, using distilled water as the mobile phase, and collect fractions every 20 mL. After tasting, it is found that the N20, N22, N23, and N24 fractions contain spicy components. Fractions containing spicy components were combined, enriched, and freeze-dried with a lyophilizer. Take 2% of the...

Embodiment 3

[0027] Enrichment Method of Pungent Components of Henan Flue-cured Tobacco Leaf X2F Extract

[0028] The Henan flue-cured tobacco X2F tobacco leaves were dried in an oven, and then pulverized into tobacco powder with a pulverizer. Add 130g of tobacco powder weighed into a 1L round bottom flask, add 700mL of ethyl acetate, reflux for 4h, filter, and concentrate the filtrate on a rotary evaporator with a pressure of 50mbar and a water bath temperature of 40°C to obtain tobacco leaf extract. Weigh 18.9 grams of Henan flue-cured tobacco leaf X2F extract, dissolve in 32.0 milliliters of distilled water, and filter. Then, load a sample to gel chromatography, with a sample volume of 9 milliliters, using distilled water as the mobile phase, and collect fractions every 20 mL. After tasting, it is found that fractions N12, 13, N16, and N17 contain spicy components. Fractions containing spicy components were combined, enriched, and freeze-dried with a lyophilizer. Take 1.5% of the enri...

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PUM

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Abstract

The invention relates to an enrichment method of a peppery component suitable for preparing an electronic cigarette liquid. The enrichment method is characterized by comprising the following steps: (1) extracting a tobacco leaf extract from tobacco leaves by virtue of a solvent; (2) weighing the tobacco leaf extract, carrying out centrifugal filtration and column chromatography separation, collecting each fraction according to the volume of eluent, and respectively marking the fractions as N1, N2,......, etc.; (3) after the fractions are tasted by at least three professional evaluation persons and the peppery fraction is determined, freeze-drying the peppery fraction by virtue of a freeze-drying machine, and enriching the peppery fraction; and (4) adding the enriched peppery fraction into propylene glycol or glycerinum, so as to prepare the electronic cigarette liquid. The enrichment method is guided through sensory evaluation, the tobacco leaf extract is separated by virtue of column chromatography, different numbers of the fractions are collected and are tasted, and the peppery fraction is freeze-dried by virtue of the freeze-drying machine and is enriched. The enrichment method has the advantages that the flow is simple, the operation is convenient, and the application range is wide; and furthermore, the obtained peppery component is derived from the tobacco leaves, so that the prepared electronic cigarette liquid has strong tobacco fragrance and can meet sensory requirements of smokers.

Description

technical field [0001] The invention belongs to the technical field of separation of tobacco products, in particular to a method for enriching spicy components suitable for preparing e-cigarette liquid. Background technique [0002] Sensory omics (sensomics) is a concept proposed by researchers in order to find the characteristic components that trigger food taste by combining separation technology and sensory evaluation technology (Angew. Chem. Int. Ed., 2011, 50, 2220-2242). At present, the most researched methods of sensory-omics are the olfactory components in natural products that trigger or regulate aroma and the taste components of sweetness, umami, bitterness, and sourness, which are widely used in food analysis. However, this method Application in tobacco leaf analysis has not been reported yet. For tobacco leaves, the impact of spiciness on sensory quality is very important. At present, a lot of research has been done on aroma components in tobacco leaves at home...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A24B15/24A24F47/00
Inventor 鲍峰玉刘俊辉范艺宽张启东刘克建柴国璧张海燕
Owner HENAN AGRICULTURAL UNIVERSITY
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