Enrichment method of peppery component suitable for preparing electronic cigarette liquid
An e-cigarette liquid and spicy technology, which is applied in the fields of tobacco, application, and tobacco processing, can solve the problems of large differences in sensory evaluation and consumer dissatisfaction, and achieve the effects of wide application range, satisfying sensory requirements, and convenient operation
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Embodiment 1
[0021] Enrichment method of spicy components of C3F extract from flue-cured tobacco leaves in Henan
[0022] The Henan flue-cured tobacco C3F tobacco leaves are dried in an oven, and then pulverized into tobacco powder with a pulverizer. Add 100g of tobacco powder weighed into a 1L round bottom flask, add 600mL of 75% ethanol, reflux for 4h, cool to room temperature, filter, and concentrate the filtrate on a rotary evaporator with a pressure of 50mbar and a water bath temperature of 40°C to obtain tobacco leaf extract . Weigh 15.0 g of Henan flue-cured tobacco leaf C3F extract, dissolve it in 30.0 ml of distilled water, and filter. Then, load a sample to gel chromatography, sample loading 10 milliliters, take distilled water as mobile phase, every 20 mL fractions are collected, find after tasting that N6, N7, N8, N13, N14 fractions contain spicy components. Fractions containing spicy components were combined, enriched, and freeze-dried with a lyophilizer. Dissolve the enric...
Embodiment 2
[0024] Enrichment method of spicy components in B2F extract of Henan flue-cured tobacco leaves
[0025] The B2F tobacco leaves of Henan flue-cured tobacco were dried in an oven, and then pulverized into tobacco powder with a pulverizer. Add 100g of tobacco powder weighed into a 1L round bottom flask, add 600mL of absolute ethanol, reflux for 4 hours, filter, and concentrate the filtrate on a rotary evaporator with a pressure of 50mbar and a water bath temperature of 40°C to obtain tobacco leaf extract. Weigh 3.0 g of Henan flue-cured tobacco leaf B2F extract, dissolve it in 6.0 ml of distilled water, and filter. Then, load a sample to gel chromatography, with a sample volume of 6 milliliters, using distilled water as the mobile phase, and collect fractions every 20 mL. After tasting, it is found that the N20, N22, N23, and N24 fractions contain spicy components. Fractions containing spicy components were combined, enriched, and freeze-dried with a lyophilizer. Take 2% of the...
Embodiment 3
[0027] Enrichment Method of Pungent Components of Henan Flue-cured Tobacco Leaf X2F Extract
[0028] The Henan flue-cured tobacco X2F tobacco leaves were dried in an oven, and then pulverized into tobacco powder with a pulverizer. Add 130g of tobacco powder weighed into a 1L round bottom flask, add 700mL of ethyl acetate, reflux for 4h, filter, and concentrate the filtrate on a rotary evaporator with a pressure of 50mbar and a water bath temperature of 40°C to obtain tobacco leaf extract. Weigh 18.9 grams of Henan flue-cured tobacco leaf X2F extract, dissolve in 32.0 milliliters of distilled water, and filter. Then, load a sample to gel chromatography, with a sample volume of 9 milliliters, using distilled water as the mobile phase, and collect fractions every 20 mL. After tasting, it is found that fractions N12, 13, N16, and N17 contain spicy components. Fractions containing spicy components were combined, enriched, and freeze-dried with a lyophilizer. Take 1.5% of the enri...
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