Purposes of icaritin in preparing medicaments for inhibiting liver cancer stem cells

A liver cancer stem cell, liver cancer inhibition technology, applied in drug combinations, antitumor drugs, pharmaceutical formulations, etc., can solve the problems of no research, no tumor stem cells, etc., and achieve the effect of curing liver cancer

Inactive Publication Date: 2015-12-02
BEIJING SHENOGEN PHARMA GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] In the prior art, there is no further research on other targets other than ER-α36, and there is no research on cancer stem cells, especially liver cancer stem cells.

Method used

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  • Purposes of icaritin in preparing medicaments for inhibiting liver cancer stem cells
  • Purposes of icaritin in preparing medicaments for inhibiting liver cancer stem cells
  • Purposes of icaritin in preparing medicaments for inhibiting liver cancer stem cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Preparation of Alcoradine

[0060] Acradine, also known as icariin, is extracted and separated from the traditional Chinese medicine Epimedium.

[0061] The preparation method of Epimedium is disclosed in the patent whose publication number is CN101302548. In the method, icariin is used as a raw material, hydrolyzed by β-glucosidase, the precipitate obtained by centrifuging the hydrolyzed product is dissolved in acetone, and the supernatant is obtained by centrifuging and filtering. Then the supernatant liquid obtained by centrifugation is recrystallized with water to obtain the pure Epimedium. The icariin in the present invention was purchased from Shaanxi Jiahe Plant Chemical Co., Ltd., with a purity of 90%.

Embodiment 2

[0063] Detection of the inhibition of alcladin on hepatoma cell Hep-12 and normal hepatocyte L02

[0064] L02 cells are human normal liver cells

[0065] Experimental steps: cell culture: first Hep-12 liver cancer cells and L02 cells were cultured in amino acid and glucose medium (i.e. DMEM medium) containing 10% fetal bovine serum in volume concentration until the cell density was 80%; The cultured cells were divided into a phenol red-free medium containing activated carbon-treated fetal bovine serum at a volume concentration of 2.5%, and cultured for 24 hours.

[0066] Add dimethyl sulfoxide (DMSO) and alcradine: add DMSO control (i.e. DMSO solution without alcradine) and alcradine DMSO at concentrations of 5 μM, 10 μM, 15 μM and 20 μM respectively in the culture system solution, and make the final volume concentration of DMSO in each reaction system 0.1%.

[0067] Detection method: After 24 and 48 hours of drug treatment, the cell proliferation level was detected with a C...

Embodiment 3

[0077] Inhibitory Effect of Alcoradine on PCL / PRF / 5 and Huh7 Liver Cancer Stem Cell Spheroids

[0078] HCC-initiating cells can form non-adherent stem cell spheres, and there are a certain number of HCC-initiating cells in the two cell lines of PCL / PRF / 5 and Huh7.

[0079] Experimental method: cell culture: first, PCL / PRF / 5 and Huh7 cells were cultured in DMEM medium to a cell density of 80%; then the cultured cells were divided into DMEM / F12 containing 2% culture factor B27 medium.

[0080] Add dimethyl sulfoxide (DMSO) and alcradine: add DMSO control (i.e. DMSO solution without alcradine) and 3 μM, 4 μM, 5 μM, 7.5 μM and 10 μM alcradine in the culture system DMSO solution, and make the final value of DMSO volume concentration in each reaction system 0.1%.

[0081] Detection method: After 5 days of alcoladin drug treatment, observe and take pictures with a microscope, and record the number of stem cell spheres at the same time.

[0082] in conclusion:

[0083] 1. The PCL / ...

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Abstract

The invention relates to purposes of icaritin in preparing medicaments for inhibiting liver cancer stem cells. Icaritin can inhibit liver cancer stem cells by inhibiting expressions of liver cancer stem cell markers. Accordingly, the invention provides a further research direction of liver cancer targeted therapy, and it is hopeful for fundamentally curing liver cancer by inhibiting liver cancer stem cells.

Description

technical field [0001] The invention relates to the use of alcradine in the preparation of medicines for inhibiting liver cancer stem cells, which belongs to the field of medicine. Background technique [0002] Stem cells are pluripotent cells capable of unlimited self-renewal and differentiation into different mature tissue types. [0003] Cancer stem cells are one or several cell subpopulations with stem cell characteristics existing in tumor tissues. Cancer stem cells have the ability of self-replication and directional differentiation, which is the root cause of tumor growth, recurrence and metastasis, and leads to the disorder of normal organ function, and finally leads to the death of the body. Similar tumor stem cells have been found in tumors of organs such as the brain, lung, liver, colon, and prostate. Therefore, tumor stem cells are considered to be effective targets for cancer therapy. However, due to the characteristics of drug resistance, radiotherapy resista...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A61P35/00
Inventor 孟坤郭玉明王媛媛朱海和尹立
Owner BEIJING SHENOGEN PHARMA GRP
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