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Method used for breeding imidazolinone herbicide-resistant japonica rice

A technology for resistance to imidazolinone and herbicides, applied in the field of rice breeding, can solve the problems of poor comprehensive properties of materials, difficult to popularize and apply, shortening the time of materials, saving costs, and solving the problem of difficult prevention and control of weedy rice Effect

Active Publication Date: 2015-12-02
SHANGHAI ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, most of the herbicide-resistant rice materials obtained in China are obtained by natural selection or mutation, but the comprehensive properties of the obtained materials are not very good, such as yield, rice quality, etc., and it is difficult to popularize and apply them in production.

Method used

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  • Method used for breeding imidazolinone herbicide-resistant japonica rice
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  • Method used for breeding imidazolinone herbicide-resistant japonica rice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Obtaining the Japonica Rice Mutant "CL55" Resistant to Imidazolinone Herbicides

[0033] With reference to the method of Gu Jiaqing et al. (Gu Jiaqing et al., Shanghai Agricultural Journal, 2005, 21 (1): 7-11), 3000 seeds of Hudao No. 55 (crop selection of Shanghai Academy of Agricultural Sciences, passed Shanghai Variety Approval in 2011) Soak in distilled water for 24 hours. The temperature should not be too high to prevent germination. After draining the water, treat it with 0.6% EMS for 24 hours. During the treatment, keep stirring. The leaf stage was treated with the imidazolinone herbicide "Dou Xerox" water agent produced by Shandong Xianda Chemical Co., Ltd., and the treatment concentration was 8 g / mu. There was a phytotoxicity reaction 7 days after the spraying, and the seedlings' hearts and leaves began to turn yellow, most of them gradually rotted, and finally died. Two of them survived normally, and the seedlings were moved to the breeding field aft...

Embodiment 2

[0035] The experiment of the influence of the imidazolinone herbicides of embodiment 2 different concentrations on rice germination

[0036] Using "CL55" and its parent "Shangdao 55" as experimental materials, imidazolinone at different concentrations of 0.0%, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 1.0% and 1.2% were studied. Effects of similar herbicides (volume ratio) on the germination rate of rice grains. The experiment was repeated 2 times. Select 100 full seeds of each material and put them into a petri dish covered with 2 layers of absorbent paper, add different concentrations of herbicides respectively, put them in a constant temperature incubator at 31°C, and count the seed germination after 5 days (Table 1) .

[0037] Table 1

[0038]

[0039]

[0040] The results showed that when the herbicide concentration started from 0.1%, the germination rate of herbicide-resistant and non-herbicide-resistant varieties began to differ. The herbicide variety "Hudao 55" did...

Embodiment 3

[0041] The influence experiment of the herbicide of embodiment 3 different concentrations and different treatment periods on rice growth

[0042] Using "CL55" and "Shanghai Rice 55" as test materials, different concentrations of herbicides (40 g / mu, 60 g / mu, 80 g / mu, prepared in 30 kg of water) and different treatment periods (1 leaf 1 core, 2 leaves 1 core, 3 leaves 1 core) on rice growth. After soaking the seeds of the two test materials to accelerate germination, select the germinated seeds that have sprouted neatly and sow them evenly. The area of ​​the plot is 1.5m 2 . Choose a sunny day, and spray different concentrations of herbicides evenly on the surface of 1 leaf with 1 core, 2 leaves with 1 core, and 3 leaves with 1 core. After 2 days, start investigating the plant damage and dead seedlings. The results are shown in Table 2 below. Different concentrations of herbicides Effects of pesticides and different treatment periods on rice growth.

[0043] Table 2

[0044...

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Abstract

The invention discloses a method used for breeding imidazolinone herbicide-resistant japonica rice. According to the method, imidazolinone herbicide-resistant Oryza sativa subsp. japonica mutant CL55 is taken as the parent, and hybridization with a high-quality high-yield conventional japonica rice variety is carried out; conventional breeding and seedling stage herbicide resistance identification and screening technology are combined so as to breed the imidazolinone herbicide-resistant japonica rice variety. Compared with that of CL55, agronomic traits, yield, and quality of the imidazolinone herbicide-resistant japonica rice variety are improved and increased greatly. Genetically modified organism-free technology is adopted to breed the imidazolinone herbicide-resistant japonica rice variety, weed control problems of weedy rice in direct seeding rice fields can be solved, labor intensity is reduced, production cost is reduced, and the development of rice light cultivation technology of our country is promoted.

Description

technical field [0001] The invention relates to the technical field of rice breeding, in particular to a method for breeding japonica rice resistant to imidazolinone herbicides. Background technique [0002] Rice is the main food crop in my country and the world, and is the main source of calories for nearly half of the world's population. In my country, due to the lack of agricultural labor force, the rice planting method has changed from the traditional transplanting method to the direct seeding method, and the weedy rice has spread, and this phenomenon is becoming more and more serious. With the promotion and application of light-weight rice cultivation technology in the past ten years, the occurrence and damage of weedy rice have become more and more serious, almost all over the major rice-producing areas in my country, and have become one of the main problems restricting the promotion of light-weight rice cultivation technology. [0003] Weedy rice is similar to ordina...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04A01H5/00A01G7/06A01C1/00
Inventor 朴钟泽白建江杨瑞芳方军张才辉
Owner SHANGHAI ACAD OF AGRI SCI
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