Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

High-density fermentation medium and fermentation technology for forage-use enterococcus faecium

A high-density fermentation and fermentation medium technology, applied in the field of high-density fermentation medium and its fermentation process, can solve the problems of low fermentation level, high price, increased cost, etc.

Inactive Publication Date: 2015-11-25
BEIJING DAWEIJIA BIOTECH SHARE CO LTD +1
View PDF9 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Traditional lactic acid bacteria fermentation mostly uses MRS medium, which is expensive and the number of viable bacteria after fermentation is less than 1×10 9 CFU / mL, under the condition that the effective dosage of probiotics is fixed, the low fermentation level equals the increased cost in disguise

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • High-density fermentation medium and fermentation technology for forage-use enterococcus faecium
  • High-density fermentation medium and fermentation technology for forage-use enterococcus faecium
  • High-density fermentation medium and fermentation technology for forage-use enterococcus faecium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment one: the preparation of high-density seed medium and fermentation medium and the comparison of the number of fermentation viable bacteria

[0037] 1. The main components and contents of the high-density fermentation seed medium and fermentation medium of Enterococcus faecium WEI-10CGMCCNo.7746 are as follows:

[0038] Fish peptone 15-20g / L, sucrose 15-25g / L, sodium acetate trihydrate 3-7g / L, dipotassium hydrogen phosphate trihydrate 1-4g / L, ammonium citrate 1-4g / L, magnesium sulfate heptahydrate 0.05-0.4g / L, manganese sulfate monohydrate 0.02-0.2g / L, Tween 800.5-2g / L.

[0039] The ingredients and composition of the preferred seed and fermentation medium are: fish peptone 17g / L, sucrose 19g / L, sodium acetate trihydrate 5g / L, dipotassium hydrogen phosphate trihydrate 2g / L, ammonium citrate 2g / L, seven Magnesium sulfate hydrate 0.2g / L, manganese sulfate monohydrate 0.05g / L, Tween 801g / L.

[0040] 2. The preparation method of seed shake flask medium is as follo...

Embodiment 2

[0063] Embodiment two: the optimization of most suitable alkaline pH neutralizer

[0064] 1. Ingredients and preparation of seeds and fermentation medium:

[0065] The main components and contents of seeds and fermentation medium are: fish peptone 17g / L, sucrose 19g / L, sodium acetate trihydrate 5g / L, dipotassium hydrogen phosphate trihydrate 2g / L, ammonium citrate 2g / L, heptahydrate Magnesium sulfate 0.2g / L, manganese sulfate monohydrate 0.05g / L, Tween 801g / L.

[0066] The seed shake flask medium preparation method is as follows:

[0067] Weigh each component of the medium, fully dissolve, adjust the pH to 8.0 with 40% sodium hydroxide solution, adjust the volume to the required volume, and sterilize at 121°C for 20 minutes.

[0068] The fermentation tank medium preparation method is as follows:

[0069] (1) Fill 17g / L sodium hydroxide solution in the fermenter, the filling volume is 60%, 0.11-0.16Mpa sterilizes 1h, discharge liquid after cooling, empty the fermenter after ...

Embodiment 3

[0080] Embodiment three: the optimization of optimum constant pH

[0081] 1. Ingredients and preparation of seeds and fermentation medium:

[0082] The main components and contents of seeds and fermentation medium are: fish peptone 17g / L, sucrose 19g / L, sodium acetate trihydrate 5g / L, dipotassium hydrogen phosphate trihydrate 2g / L, ammonium citrate 2g / L, heptahydrate Magnesium sulfate 0.2g / L, manganese sulfate monohydrate 0.05g / L, Tween 801g / L.

[0083] The seed shake flask medium preparation method is as follows:

[0084] Weigh each component of the medium, fully dissolve, adjust the pH to 8.0 with 40% sodium hydroxide solution, adjust the volume to the required volume, and sterilize at 121°C for 20 minutes.

[0085] The fermentation tank medium preparation method is as follows:

[0086] (1) Fill 17g / L sodium hydroxide solution in the fermenter, the filling volume is 60%, 0.11-0.16Mpa sterilizes 1h, discharge liquid after cooling, empty the fermenter after rinsing fermente...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a high-density fermentation medium and a fermentation technology for forage-use enterococcus faecium. By adopting the high-density fermentation medium and an in-batch fed-batch fermentation technology provided by the invention, the highest viable count of enterococcus faecium WEI-10CGMCC No.7746 under a 50L fermentation tank scale condition can reach 2.5X10<10> CFU / mL, and the highest viable count of enterococcus faecium WEI-10CGMCC No.7746 under a 5-ton fermentation tank scale condition can reach 2.9X10<10> CFU / mL, which are respectively 34 times and 36 times of those of in-batch fermentation of the traditional MES medium under the same fermentation scale condition, while the cost of the high-density fermentation medium is obviously reduced compared with that of the MRS medium, and thus the low-cost and high-efficiency production of enterococcus faecium as the microecological preparation is realized.

Description

technical field [0001] The invention belongs to the technical field of lactic acid bacteria cultivation, and relates to a high-density fermentation medium of Enterococcus faecium for feeding and a fermentation process thereof. Background technique [0002] Enterococcus Faecium (Enterococcus Faecium) belongs to the normal flora in the intestinal tract of humans and animals, and can colonize effectively after entering the intestinal tract. The fermentation of Enterococcus faecium produces lactic acid, which is beneficial to reduce the pH of the intestinal environment and inhibit the growth of harmful bacteria. During the metabolic process of Enterococcus faecium, hydrogen peroxide, bacteriocin and other substances will be produced, which have a certain killing effect on pathogenic bacteria without drug residue. In view of the above characteristics of Enterococcus faecium, more and more manufacturers choose it for the fermentation production of probiotics. [0003] Traditiona...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/01
Inventor 乔琳姚宏明高长斌刘蕊金忠辉
Owner BEIJING DAWEIJIA BIOTECH SHARE CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com