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Application of Terreumol A in preparation of neuroprotective drug

A technology of terreumola, 1. terreumola is applied in the application field of terreumolA in the preparation of neuroprotective drugs, and can solve problems such as no compound activity report yet.

Inactive Publication Date: 2015-11-25
林天样
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There is no report on the activity of this compound

Method used

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  • Application of Terreumol A in preparation of neuroprotective drug
  • Application of Terreumol A in preparation of neuroprotective drug
  • Application of Terreumol A in preparation of neuroprotective drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Example 1: Isolation, Preparation and Structure Confirmation of Terreumol A

[0013] The preparation method of Terreumol A is the same as that reported in the literature (HighlyOxygenatedMeroterpenoidsfromFruitingBodiesoftheMushroomTricholomaterreum, J.Nat.Prod., 2013, 76, 1365-1368).

[0014] Structural confirmation: yellow crystal, molecular formula is C 25 h 34 o 8 , with an unsaturation of 8. The IR data show that the compound contains a hydroxyl group (3440cm - 1 ), the compound has ultraviolet absorption at 372 and 291nm, indicating that it contains a conjugated moiety. H NMR spectrum data δ H (ppm, DMSO-d 6 , 600MHz): H-2 (6.52, s), H-5 (3.44, d, J=15.1), H-5 (2.77, d, J=15.1), H-7 (2.85, dd, J=9.8 , 3.8), H-8 (2.27, m), H-8 (1.45, m), H-9 (2.33, dd, J=12.7, 7.3), H-9 (1.43, m), H-11 ( 3.89, m), H-15 (1.33, s), H-16 (1.38, s), 1-OH (11.97, s), 4-OH (7.85, s), 3-OCH 3 (3.96, s); C NMR spectrum data δ C (ppm, DMSO-d 6 , 600Hz): 159.2 (C, 1-C), 99.7 (CH,...

Embodiment 2

[0015] Embodiment 2: The pharmacological action test of TerreumolA

[0016] 1. Materials and Instruments

[0017] Aβ 25-35 Purchased from sigma company in the United States. TerreumolA is self-made, and the HPLC normalized purity is greater than 98%. MTT (nitroblue tetrazolium) was purchased from Amresco, USA. LDH assay kit was purchased from Nanjing Jiancheng Biological Co., Ltd. Acridine orange (AO) was purchased from Sigma, USA. Ethidium bromide (EB) was purchased from Sigma, USA. RNase enzyme was purchased from Sigma, USA. Proteinase K was purchased from sigmaAnnexin, USA. Ⅴ-FITC cell apoptosis detection kit was purchased from Nanjing Kaiji Biotechnology Development Co., Ltd. The dry powder of D-MEM / F12 medium was purchased from GibcoL, USA. Horse serum was purchased from hycLon Company in the United States. Fetal bovine serum was purchased from Hangzhou Sijiqing Biological Engineering Co., Ltd. Polylysine (PLL) was purchased from Sigma, USA.

[0018] Low-tempe...

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Abstract

The invention discloses an application of Terreumol A in preparation of a neuroprotective drug and belongs to the field of drugs. The research shows that an apoptosis model of rat chromaffin tumor cell strains PC12 cultured in vitro can be effectively established with the application of 20 mu mol / L of Abeta25-35; Terreumol A can be partially resistant to Abeta25-35 induced PC12 apoptosis and can be further researched and developed to prepare the neuroprotective drug.

Description

technical field [0001] The present invention relates to a new application of compound Terreumol A, in particular to the application of Terreumol A in the preparation of neuroprotective drugs. Background technique [0002] XiaYin et al. isolated and purified the compound Terreumol A for the first time, and published the results in the famous Journal of Natural Products (Highly Oxygenated Meroterpenoids from Fruiting Bodies of the Mushroom Tricholomaterreum, J. Nat. Prod., 2013, 76, 1365-1368). [0003] There is no activity report of this compound yet. Contents of the invention [0004] The purpose of the present invention is to provide a medical application of TerreumolA. [0005] The above-mentioned purpose is achieved through the following technical solutions: [0006] Application of TerreumolA in the preparation of neuroprotective drugs, the chemical structural formula of TerreumolA is as follows, [0007] Description of drawings [0008] figure 1 : MTT metaboli...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/336A61P25/00
Inventor 林天样
Owner 林天样
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