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Application of Pedinophyllol B in preparation of vascular protective drug

A technology of pedinophyllolb, 1. pedinophyllolb is applied in the application field of Pedinophyllol B in the preparation of vascular protective drugs, and can solve problems such as no reports of compound activity yet.

Inactive Publication Date: 2015-11-25
林天样
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There is no report on the activity of this compound

Method used

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  • Application of Pedinophyllol B in preparation of vascular protective drug
  • Application of Pedinophyllol B in preparation of vascular protective drug
  • Application of Pedinophyllol B in preparation of vascular protective drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Example 1: Isolation, Preparation and Structure Confirmation of PedinophyllolB

[0010] The preparation method of PedinophyllolB is the same as that reported in the literature (HighlyOxygenatedent-Pimarane-TypeDiterpenoids from the Chinese Liverwort Pedinophyllum interruptum and Their Allelopathic Activities, J.Nat.Prod., 2013, 76, 1647-1653).

[0011] Structural confirmation: the molecular formula is C 20 h 28 o 3 , with an unsaturation of 7. H NMR spectrum data δ H (ppm, DMSO-d 6 , 500MHz): H-2 (2.82, dt, J=4.9, 13.3), H-2 (2.23, m), H-3 (1.79-1.83, m), H-5 (2.39, dd, J=3.8 , 12.6), H-6 (2.28, m), H-7 (6.80, d, J=4.8), H-11 (1.96, dt, J=17.9, 2.5), H-11 (2.36, m), H-12 (1.73, m), H-12 (2.24, m), H-15 (5.70, dd, J=10.7, 17.6), H-16 (5.07, d, J=10.7), H-16 ( 4.83, d, J=17.6), H-17 (1.23, s), H-18 (1.02, s), H-19 (1.19, s), H-20 (1.19, s); C NMR data δ C (ppm, DMSO-d 6 , 150Hz): 218.5 (C, 1-C), 37.1 (CH 2 , 2-C), 41.5 (CH 2 , 3-C), 32.7(C, 4-C), 44.2(CH, 5-C)...

Embodiment 2

[0012] Embodiment 2: Pharmacological action test of Pedinophyllol B

[0013] 1. Materials and Instruments

[0014] Vascular endothelial cells (ECV-304) were donated by the Department of Immunology, School of Medicine, Shandong University. PedinophyllolB is self-made, and the HPLC normalized purity is greater than 98%. RPMI-1640 dry powder medium and trypsin were purchased from Gibeo, USA. Fetal bovine serum was purchased from Tianjin TBD Company. MTT, agarose, and AilllexinV-FITC were purchased from Sigma, USA. LDH, MDA, SOD, GSH-Px assay kits were purchased from Nanjing Jiancheng Bioengineering Institute. DMSO was purchased from China Pharmaceutical (Group) Shanghai Chemical Reagent Co., Ltd. Hematoxylin was purchased from Fuzhou Maixin Biotechnology Co., Ltd. Rhodamine123 was purchased from Solarbic Corporation of the United States. The positive drug ligustrazine was purchased from Shanghai Yuanye Biotechnology Co., Ltd.

[0015] Inverted optical microscope (Japan Ol...

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Abstract

The invention discloses an application of Pedinophyllol B in preparation of a vascular protective drug and belongs to the field of drugs. MTT (methyl thiazolyl tetrazolium) assay and LDH (lactic dehydrogenase) activity detection prove that Pedinophyllol B has an protective effect on H2O2 oxidative damage ECV-304 cells; measurement for MDA (malondialdehyde) content, SOD (superoxide dismutase) activity and GSH-Px (glutathion peroxidase) activity of a cell supernatant proves that Pedinophyllol B has the free radical scavenging property and the antioxidant property of active oxygen and can be further researched and developed to prepare the vascular protective drug.

Description

technical field [0001] The present invention relates to a new application of compound PedinophyllolB, in particular to the application of PedinophyllolB in the preparation of neuroprotective drugs. Background technique [0002] NaLiu et al. isolated and purified the compound PedinophyllolB for the first time, and published the results in the famous Journal of Natural Products (Highly Oxygenated-Pimarane-Type Diterpenoids from the Chinese Liverwort Pedinophyllum interruptum and Their Allelopathic Activities, J. Nat. Prod., 2013, 76, 1647-1653). [0003] There is no activity report of this compound yet. Contents of the invention [0004] The purpose of the present invention is to provide a medical application of PedinophyllolB. [0005] The above-mentioned purpose is achieved through the following technical solutions: [0006] Application of PedinophyllolB in the preparation of vascular protective drugs, the chemical structural formula of PedinophyllolB is as follows, [...

Claims

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Application Information

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IPC IPC(8): A61K31/122A61P9/14A61P39/06
Inventor 林天样
Owner 林天样
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