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Microbial combined insecticide and preparation method thereof

A technology of pesticides and microorganisms, which is applied in the field of microbial combination pesticides and their preparation, and can solve the problems of the use and improper handling of synthetic highly toxic pesticides.

Inactive Publication Date: 2015-11-25
段一皋 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, many non-fatal incidents occurred due to the use and mishandling of highly toxic synthetic pesticides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Preparation of microbial combination insecticide (unit: kg):

[0068] Step 1: Strain cultivation: According to the name and serial number of the strains, the Agricultural Microbiology Center of the China Microbiology Preservation Committee bought back 18 sand tube strains in three categories; the three types of microorganisms are fungi, actinomycetes and bacteria.

[0069] There are 3 types of fungi, namely: (1) metarhizium aunisopliae (metschn) sorok scarab anisopliae ACCC-30101 ← Institute of Soil and Fertilizer, Chinese Academy of Agricultural Sciences ← Institute of Microbiology, Chinese Academy of Sciences AS3. Separation from winged moth larvae; used to prevent corn borers, scarabs, and winged moths. (2) Paecilomyces farinosus (Dicks.) Brown powder Paecilomyces ACCC-30190 ← Institute of Soil and Fertilizer, Chinese Academy of Agricultural Sciences ← Wu Jinwen, Beijing Forestry University; original number: 8601, control pine caterpillar. (3) Paecilomyceslilacinus ...

Embodiment 2

[0076] Preparation of microbial combination insecticide (unit: kg):

[0077] Step 1: Strain cultivation: According to the name and serial number of the strain, the Agricultural Microbiology Center of China Microbiology Preservation Committee repurchases fungi, actinomycetes, and bacterial strains;

[0078] 2 fungi: (1) metarhizium aunisopliae (metschn) sorok scarab genus ACCC—30101 ← Institute of Soil and Fertilizer, Chinese Academy of Agricultural Sciences ← Institute of Microbiology, Chinese Academy of Sciences AS3.3675 ← Sand Control Institute of Shanxi Province, from the larvae of the poplar moth Separation on the top; used to prevent corn borers, scarabs, and winged moths.

[0079] (2) Paecilomyces farinosus (Dicks.) Brown powder Paecilomyces ACCC - 30190 ← Institute of Soil and Fertilizer, Chinese Academy of Agricultural Sciences ← Wu Jinwen, Beijing Forestry University; original number: 8601, control pine caterpillar.

[0080] 3 actinomycetes, respectively: (1) Strepto...

Embodiment 3

[0086] Preparation of microbial combination insecticide (unit: kg):

[0087] Step 1: Strain cultivation: According to the name and serial number of the strain, the Agricultural Microbiology Center of the China Microbiology Collection Committee repurchases the strains of fungi and actinomycetes in sandy soil tubes;

[0088] There are 3 types of fungi, namely: (1) metarhizium aunisopliae (metschn) sorok scarab anisopliae ACCC——30101 ← Institute of Soil and Fertilizer, Chinese Academy of Agricultural Sciences ← Institute of Microbiology, Chinese Academy of Sciences AS3.3675 ← Sand Control Institute of Shanlin, Shaanxi, from poplar Separation from the larvae of the penetrating moth; used to prevent corn borers, scarabs, and penetrating moths.

[0089] (2) Paecilomyces farinosus (Dicks.) Brown powder Paecilomyces ACCC - 30190 ← Institute of Soil and Fertilizer, Chinese Academy of Agricultural Sciences ← Wu Jinwen, Beijing Forestry University; original number: 8601, control pine cat...

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Abstract

The invention discloses a microbial combined insecticide and its preparation method. The microbial combined insecticide is a concentrated cenobium preparation prepared from two or three strains selected from 40-60 parts by weight of fungi, 15-25 parts by weight of actinomycetes and / or 20-40 parts by weight of bacteria. The preparation method comprises the following steps: respectively culturing various strains in a routine way to obtain strain culture pure liquids; enlarged culture of bacteria solutions: mixing the strain culture pure liquids in proportion to prepare mixed bacteria solutions; mixing the mixed bacteria solutions according to the weight ratio; culturing with a culture solution and fermenting to obtain a product with high concentration of 2 billions / ml and a solid product. The microbial combined insecticide provided by the invention has the characteristic of poisoning different insects and insect species, has a strong poisoning effect on hundreds of pests such as scarab, mosquito, nematode, Lepidoptera, coleoptera, Hymenoptera and the like, has advantages of fast speed, and wide range and thorough poisoning. In comparison with insecticidal efficacy of an insecticide produced by the use of a single strain, insecticidal efficacy of the microbial combined insecticide is raised by 51% and above.

Description

technical field [0001] The invention relates to a microbial insecticide, in particular to a microbial combination insecticide and a preparation method thereof. Background technique [0002] Since the 20th century, the world's population has grown rapidly, and the total demand for food has also increased. The "petrochemical agriculture" invented by people, chemical fertilizers and pesticides have helped humans obtain a large amount of food resources and promoted human development and progress. [0003] At present, there are 7.5 billion people in the world, which will reach 9 billion after 2050, and will approach the peak of 10.5 billion by the end of this century. Therefore, human beings' requirements for food will increase significantly. Man's reliance on "oil agriculture" is also increasing. [0004] According to the statistics of authoritative departments in the world, there are more than 11 million kinds of artificially synthesized chemical substances in the world at p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01P7/04A01N63/00
Inventor 段一皋王羲元
Owner 段一皋
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